Effect of Blueberry Supplementation on Liver Enzymes, Muscle Injury and Respiratory Parameter in Football Athletes: A Eight-Week Training Study

NCT07170878 | Completed DMC

• 1 other identifier: E-95674917-108.99-164965
• Study Type: interventional
• Target: 36
• Locations: 1 country
• Sites: 1

Brief Summary

Abstract Background and objective: Regular Exercise causes physiological changes in the body. As a result, its impact on liver enzymes and muscle injury is unknown. The purpose of this study was to look into the effect of blueberry supplement taken in addition to football training on athlete muscle injury, liver enzymes and respiratory parameter. Methods: The present study comprised 36 male football players who volunteered for the study. The participants were divided into three groups: a Control Group (CG) (n=12), which performed only football training; a Supplementation Group (EG) (n=12), which received blueberry supplementation in addition to football training; and a Supplementation + Exercise Group (SEG) (n=12), which received blueberry supplementation and 20 minutes of exercise in addition to football training. A eight-week training program was implemented to the athletes three days per week in our study. The athletes' blood was drawn twice before and after training. The athletes' markers of muscle injury and liver enzymes were measured as a result of blood samples taken. The SPSS package program was used to examine the study's data.

Conditions

Performance

Keywords

Respiratory Parameter; Liver Enzymes; Muscle Damage; Blueberry; Football

Outcome Measures

Primary Outcomes (6)

• Change in muscle injury markers

  Change in muscle injury markers (CK) from baseline to 8 weeks.

  8 weeks

• Change in liver enzyme levels

  Change in liver enzyme levels (ALT) from baseline to 8 weeks.

  8 weeks

• Change in muscle injury markers

  Change in muscle injury markers (CK-MB) from baseline to 8 weeks.

  8 weeks

• Change in liver enzyme levels

  Change in liver enzyme levels (AST) from baseline to 8 weeks.

  8 weeks

• Change in liver enzyme levels

  Change in liver enzyme levels (LDH) from baseline to 8 weeks.

  8 weeks

• Change in liver enzyme levels

  Change in liver enzyme levels (ALP) from baseline to 8 weeks.

  8 weeks

Secondary Outcomes (5)

• Change in pulmonary function parameters

  8 weeks

• Change in maximal inspiratory pressure

  8 weeks.

• Change in pulmonary function parameters

  8 weeks

• Change in pulmonary function parameters

  8 weeks

• Change in pulmonary function parameters

  8 weeks

Study Arms (3)

Supplementation Group (EG)

EXPERIMENTAL

group given blueberry supplement in addition to soccer training

Dietary Supplement: Blueberry Supplementation; Dietary Supplement: Supplementation Group

CONTROL

NO INTERVENTION

The research group (Control Group (CG) (n:12): soccer training group;

Reinforcement+Exercise (SEG)

ACTIVE COMPARATOR

Reinforcement+Exercise (SEG) (n:12): the group who was given additional blueberry supplement in soccer training and 20 minutes of resistance band exercise)

Dietary Supplement: Blueberry Supplementation; Dietary Supplement: Supplementation Group

Interventions

Blueberry Supplementation DIETARY_SUPPLEMENT

The research group (Control Group (CG) (n:12): soccer training group; Supplementation Group (EG) (n:12): group given blueberry supplement in addition to soccer training; Reinforcement+Exercise (SEG) (n:12): the group who was given additional blueberry supplement in soccer training and 20 minutes of resistance band exercise) consisted of 36 male athletes in the football branch and regularly participating.

Reinforcement+Exercise (SEG); Supplementation Group (EG)

Supplementation Group DIETARY_SUPPLEMENT

Supplementation + Exercise

Reinforcement+Exercise (SEG); Supplementation Group (EG)

Eligibility Criteria

• Age: 14 Years - 18 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)

You may qualify if:

• a) at least two years of active football experience, (b) age between 14 and 18 years, (c) good general health with no known respiratory or cardiovascular conditions, (d) provision of written informed consent, and (e) Playing football in Elazığspor.

You may not qualify if:

• history of lung disease or current upper respiratory tract infections;
• existing injury problems;
• use of medications that could affect respiratory;
• current smokers or individuals with a history of smoking within the past year;
• participation in similar studies within the last six months.

Sponsors & Collaborators

• Gümüşhane Universıty: lead

Study Sites (1)

Gumushane Univetsity

Gümüşhane, Kelkit, 29600, Turkey (Türkiye)

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: SUPPORTIVE CARE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: ASSOC. PROF.

Model Details: Participants visited the laboratory 3 times. At the first visit, information about the study was given and try tests of the tests and training to be used were carried out. At the second visit, measurements of respiratory function and respiratory muscle strength and blood samples were taken. At the final visit, after 8 weeks of training, the measurements taken at the second visit were repeated. Eight Weeks Reinforcement Period Blueberry powder supplementation (26 g or \~1 cup fresh blueberry equivalent per day) was provided in 25 vacuum-sealed packets consisting of low bush wild blueberries (Vaccinium angustifolium Ait) sourced from Allen's Blueberries (Ellsworth, ME) and freeze-dried by Future Ceuticals (Momence, IL). Study participants consumed blueberry powders mixed with water, fruit juice, yoghurt or other products in the first meal of the day before each workout for 8 weeks.

Study Record Dates

• First Submitted: March 4, 2025
• First Posted: September 12, 2025
• Study Start: January 5, 2025
• Primary Completion: February 20, 2025
• Study Completion: February 20, 2025
• Last Updated: September 30, 2025

Record last verified: 2025-09

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP, ICF

Locations

TU (1)