Acute Effects of Beetroot Juice and Caffeine Co-ingestion on Lower-limb Strength and Muscular Endurance in Females

NCT06758323 | Completed

• 1 other identifier: IMIBIC_BRJ_CAF
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. The main questions it aims to answer are:

• Does the co-ingestion of beetroot juice and caffeine enhance lower-limb strength and muscular endurance more than either substance alone or a placebo?
• How do the acute effects of beetroot juice interact with caffeine on physical performance? The study was conducted using a randomized, crossover design to evaluate the following four conditions: Beetroot juice with placebo Caffeine with placebo Beetroot juice with caffeine Placebo only Each participant completed all conditions, with a sufficient washout period between sessions to eliminate potential carryover effects. This design allowed for a direct comparison of the acute effects of each supplementation strategy on lower-limb strength and muscular endurance. Participant Protocol Participants consumed their assigned supplement on a single testing day for each condition. After supplementation, they performed standardized tests to measure lower-limb strength and muscular endurance. Progress was monitored, and data from all conditions were collected to assess the acute effects of each intervention.

Conditions

Beetroot Juice; Caffeine; Placebo; Beetroot Juice with Caffeine Co-ingestion

Keywords

beetroot juice; nitrate; caffeine; resistance training; dietary supplement; female

Outcome Measures

Primary Outcomes (1)

• Change of height reached in jump

  Evaluated through Countermovement jump test with a recovery period of 45 seconds between jumps. The height reached was recorded in centimeters.

  Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)

Secondary Outcomes (3)

• Movement velocity

  Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)

• Muscular endurance test

  Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)

• Rating of perceived exertion (RPE).

  Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)

Study Arms (4)

COM

EXPERIMENTAL

Beetroot juice combined with caffeine

Dietary Supplement: Beetroot juice combined with caffeine (COM)

CAF

ACTIVE COMPARATOR

Beetroot juice placebo with caffeine

Dietary Supplement: Beetroot juice placebo with caffeine (CAF)

BRJ

ACTIVE COMPARATOR

Beetroot juice with caffeine placebo

Dietary Supplement: Beetroot juice with caffeine placebo (BRJ)

PLA

PLACEBO COMPARATOR

Beetroot juice placebo with caffeine placebo

Other: Beetroot placebo with caffeine placebo (PLA)

Interventions

Beetroot juice combined with caffeine (COM) DIETARY_SUPPLEMENT

* 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session * 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session

COM

Beetroot juice placebo with caffeine (CAF) DIETARY_SUPPLEMENT

\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session

CAF

Beetroot juice with caffeine placebo (BRJ) DIETARY_SUPPLEMENT

\- 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session

BRJ

Beetroot placebo with caffeine placebo (PLA) OTHER

\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session

PLA

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Minimal experience of six months of strength training
• Be familiarized with the exercise test: counter movement jump (CMJ), barbell back squats, leg press and knee extension.

You may not qualify if:

• Supplementation consumption
• Sports injuries

Sponsors & Collaborators

• Jose Manuel Jurado Castro: lead

Study Sites (1)

Edificio IMIBIC Avda Menéndez Pidal s/n

Córdoba, 14004, Spain

Location

MeSH Terms

Interventions

Caffeine

Intervention Hierarchy (Ancestors)

Xanthines; Alkaloids; Heterocyclic Compounds; Purinones; Purines; Heterocyclic Compounds, 2-Ring; Heterocyclic Compounds, Fused-Ring

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: OTHER
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Principal Investigator

Model Details: Double-Blind Crossover Randomized Trial

Study Record Dates

• First Submitted: December 26, 2024
• First Posted: January 3, 2025
• Study Start: August 1, 2024
• Primary Completion: December 26, 2024
• Study Completion: December 26, 2024
• Last Updated: January 6, 2025

Record last verified: 2025-01

Data Sharing

• IPD Sharing: Will not share

Locations

ES (1)