Salivary Proteome in Response to Non-surgical Periodontal Treatment

NCT05952895 | Completed

• 1 other identifier: Proteome
• Study Type: observational
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to investigate salivary proteome changes in periodontitis patients before and after non-surgical treatment. Ten systemically healthy and non-smoker individuals with stage III, grade C periodontitis underwent non-surgical periodontal treatment. Saliva was collected at baseline, and one and six months post-treatment. Whole-mouth plaque and gingival index, probing depth, bleeding on probing and clinical attachment loss were measured. The saliva proteome was investigated by label-free quantitative proteomics. Normalized protein intensities were measured and protein changes were modeled over time with significant protein regulation considered at false discovery rate (FDR)\<0.05.

Conditions

Periodontitis

Keywords

Periodontitis; Saliva; Proteome

Outcome Measures

Primary Outcomes (1)

• Saliva protein content change

  pg/ml

  Change from baseline to 1 month and 6 months after treatment

Study Arms (1)

Stage III, Grade C Periodontitis

Conventional quadrant-wise scaling and root planing (SRP)

Procedure: Conventional quadrant-wise scaling and root planing (SRP)

Interventions

Conventional quadrant-wise scaling and root planing (SRP) PROCEDURE

SRP was conducted at baseline, after saliva collection and periodontal assessment, starting with the upper right quadrant and continuing clockwise over four visits at weekly intervals. SRP was performed under local anesthesia by the same periodontist (B.A.) by use of ultrasonic instruments (Mini Piezon, EMS, Nyon, CH) and manual periodontal curettes (Gracey curets, scaler Hu-Friedy, Chicago, IL). All present teeth were instrumented until the root surface was visually and tactilely clean and smooth. Self-performed plaque control measures consisted of toothbrushing using the modified Bass technique with a medium toothbrush and a regular toothpaste with fluoride twice a day and interdental cleaning using dental floss and/or interdental brushes once a day.

Stage III, Grade C Periodontitis

Eligibility Criteria

• Age: 32 Years - 43 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

A total of 10 systemically healthy and non-smoker individuals with stage III, grade C periodontitis (5 males/5 females; aged 32 to 43 years) were consecutively included.

You may qualify if:

• Non-smokers
• Having at least 20 natural teeth.

You may not qualify if:

• Having any chronic inflammatory or immunological conditions such as diabetes mellitus, cardiovascular diseases, rheumatoid arthritis and mucocutaneous diseases,
• Pregnant or lactating
• Intake of antimicrobials, anti-inflammatory drugs and immunosuppressive agents within the last 6 months
• Non-surgical/surgical periodontal therapy within 12 months
• Having orthodontic appliances, removable partial dentures and restorative and endodontic treatment requirements.

Sponsors & Collaborators

• Aydin Adnan Menderes University: lead

Study Sites (1)

Adnan Menderes University, Faculty of Dentistry, Department of Periodontology

Aydin, 09100, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Unstimulated whole saliva

MeSH Terms

Conditions

Periodontitis

Interventions

Root Planing

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Intervention Hierarchy (Ancestors)

Dental Scaling; Dental Prophylaxis; Periodontics; Dentistry; Subgingival Curettage; Preventive Dentistry

Study Officials

• Angelika Silbereisen

  Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden

  PRINCIPAL INVESTIGATOR

• Kai Bao

  Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden

  PRINCIPAL INVESTIGATOR

• Witold Wolski

  Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Switzerland

  PRINCIPAL INVESTIGATOR

• Paolo Nanni

  Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Switzerland

  PRINCIPAL INVESTIGATOR

• Laura Kunz

  Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Switzerland

  PRINCIPAL INVESTIGATOR

• Beral Afacan

  Department of Periodontology, Faculty of Dentistry, Adnan Menderes University, Aydın, Turkey

  PRINCIPAL INVESTIGATOR

• Gülnur Emingil

  Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey

  PRINCIPAL INVESTIGATOR

• Nagihan Bostanci

  Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: July 6, 2023
• First Posted: July 19, 2023
• Study Start: July 1, 2018
• Primary Completion: June 30, 2019
• Study Completion: December 30, 2019
• Last Updated: July 19, 2023

Record last verified: 2023-07

Locations

TU (1)