Effect of Long-lasting Adaptation to Endurance and Speed-power Training on Plasma Free Amino Acids Concentration

NCT05672758 | Unknown DMC

• 1 other identifier: OPUS 14 2017/27/B/NZ7/02828
• Study Type: observational
• Target: 58
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this observational study was to detect the long-term effect of two different training modalities - speed-power and endurance training - on changes in plasma free amino acid (PFAA) concentration at rest, during graded exercise and post-exercise recovery period. It was assumed that these training modalities cause different amino acids concentration in human blood depending on long-term sport specialization and predominant exercise type (the contribution of high-intensity exercise related to anaerobic metabolism). The hypotheses were:

1. 1.highly-trained speed-power have higher concentrations of PFAA than endurance athletes;
2. 2.PFAA concentration varies with the change in training loads in a one-year training cycle. Higher PFAA concentrations is expected in training phases with larger contribution of high-intensity exercise;
3. 3.PFAA concentration per 1 kg muscle mass differ between speed-power and endurance athletes.

Conditions

Healthy Athletes Aged 18-35 Years

Keywords

free plasma amino acids; competitive athletes; sprint training; endurance training; mass spectrometry

Outcome Measures

Primary Outcomes (2)

• absolute Plasma Free Amino Acids Concentration (42 amino acids)

  µmol/L

  4 times in a 1-year training cycle: at rest, during graded exercise (every 3 min), and during recovery (5, 10, 15, 20, and 30 min)

• relative Plasma Free Amino Acids Concentration (42 amino acids)

  µmol/L/kg muscle mass

  4 times in a 1-year training cycle: at rest, during graded exercise (every 3 min), and during recovery (5, 10, 15, 20, and 30 min)

Secondary Outcomes (9)

• absolute Skeletal Muscle Mass (aSMM)

  4 times in a 1-year training cycle

• relative Skeletal Muscle Mass (rSMM)

  4 times in a 1-year training cycle

• absolute Lean Body Mass (aLBM)

  4 times in a 1-year training cycle

• relative Lean Body Mass (rLBM)

  4 times in a 1-year training cycle

• absolute Fat Mass (aFM)

  4 times in a 1-year training cycle

Other Outcomes (22)

• White Blood Cells

  4 times in a 1-year training cycle

• Red Blood Cells

  4 times in a 1-year training cycle

• Hematocrit

  4 times in a 1-year training cycle

Study Arms (3)

Sprint-Trained Athletes

Highly trained track sprinters at the national or international level aged 18-35 years with sport experience 5-10 years; structured periodized training

Behavioral: Sprint training

Endurance-Trained Athletes

Highly trained long-distance runners and triathletes at the national or international level aged 18-35 years with sport experience 5-10 years; structured periodized training

Behavioral: Endurance training

Amateur/Recreational Athletes (Controls)

Individuals participating in amateur non-professional sport aged 18-35 years with activity experience 5-10 years; unstructured non-periodized training

Behavioral: Recreational Training

Interventions

Sprint training BEHAVIORAL

One-year cycle including specific sprint-oriented athletic training

Sprint-Trained Athletes

Endurance training BEHAVIORAL

One-year cycle including specific endurance-oriented athletic training

Endurance-Trained Athletes

Recreational Training BEHAVIORAL

One year of nonspecific recreational training

Amateur/Recreational Athletes (Controls)

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Highly trained competitive athletes aged 18-35 years with longer competitive sport experience, specialized in sports of different character - speed-power (sprinters) vs endurance (triathletes, distance runners). Amateur/recreational athletes participating in training on a regular basis.

You may qualify if:

• highly trained sprint- and endurance-trained athletes
• national or international performance level
• current participation in training programs organized by professional sports clubs or national team (licence) for at least 5 years
• current medical eligibility for competitive sport
• regular recreational activity for at least 5 years (preferred endurance disciplines)
• participation in amateur competition
• good health status
• non-smoking

You may not qualify if:

• untrained individuals
• athletes that do not meet the above criteria for participation in professional sport
• injured athletes or those who are not able or willing to participate for other reasons
• inactive/sedentary individuals
• medical contraindications to high-intensity exercise and testing
• injured individuals or those who are not able or willing to participate for other reasons
• current smokers or heavy past smokers

Sponsors & Collaborators

• Poznan University of Physical Education: lead
• Poznan University of Medical Sciences: collaborator
• National Science Centre, Poland: collaborator

Study Sites (1)

Poznan University of Physical Education

Poznan, Wielkoolska, 60-687, Poland

Location

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MeSH Terms

Interventions

High-Intensity Interval Training; Endurance Training

Intervention Hierarchy (Ancestors)

Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities

Study Officials

• Krzysztof Kusy, PhD

  Poznan University of Physical Education

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associated Professor

Study Record Dates

• First Submitted: January 3, 2023
• First Posted: January 5, 2023
• Study Start: July 31, 2018
• Primary Completion: July 31, 2023
• Study Completion: July 31, 2023
• Last Updated: January 9, 2023

Record last verified: 2023-01

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP, ICF
• Time Frame: Starting 1 year after publication of the data
• Access Criteria: Scientists specialized in amino acids and training research on request (kusy@awf.poznan.pl). Principal investigator will review the requests

Locations

PL (1)