NCT04136457

Brief Summary

The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
72

participants targeted

Target at P50-P75 for not_applicable

Timeline
Completed

Started Oct 2019

Longer than P75 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

October 21, 2019

Completed
2 days until next milestone

First Posted

Study publicly available on registry

October 23, 2019

Completed
7 days until next milestone

Study Start

First participant enrolled

October 30, 2019

Completed
4.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 28, 2023

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

November 28, 2023

Completed
Last Updated

December 27, 2023

Status Verified

December 1, 2023

Enrollment Period

4.1 years

First QC Date

October 21, 2019

Last Update Submit

December 19, 2023

Conditions

Skeletal Muscle Physiology

Outcome Measures

Primary Outcomes (2)

  • Skeletal muscle proteome signature

    Pathway enrichment analysis of the muscle proteome

    Change from baseline at week 6 or 8 depending on the intervention

  • Anabolic signaling

    Western blotting of proteins related to anabolic signaling

    Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training

Secondary Outcomes (6)

  • Skeletal muscle mitochondrial respiration

    Change from baseline at week 6 following endurance and sprint training

  • Maximal oxygen consumption

    Change from baseline at week 6 following endurance and sprint training

  • Body composition

    Change from baseline at week 6 or 8 depending on the intervention

  • Myonuclei

    Change from baseline at week 8 following resistance training

  • Satellite cells

    Change from baseline at week 8 following resistance training

  • +1 more secondary outcomes

Other Outcomes (1)

  • Skeletal muscle mitochondrial supercomplexes

    Change from baseline at week 6 following endurance and sprint training

Study Arms (3)

Endurance

EXPERIMENTAL

Endurance training

Other: Endurance training

Resistance

EXPERIMENTAL

Resistance training

Other: Resistance training

Sprint

EXPERIMENTAL

Sprint training

Other: Sprint training

Interventions

Endurance trainingOTHER

Subjects perform a period of endurance training

Endurance
Resistance trainingOTHER

Subjects perform a period of resistance training

Resistance
Sprint trainingOTHER

Subjects perform a period of sprint training

Sprint

Eligibility Criteria

Age18 Years - 45 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy men and women
  • VO2max \> 55 mL/min/kgLM for men and \> 50 for women (only applicable for endurance and sprint training interventions)
  • BMI \< 26

You may not qualify if:

  • Chronic disease deemed by the medical doctor to pose a risk or to affect study outcomes
  • smoking
  • Use of prescription medicine deemed by the medical to affect study outcomes
  • Pregnancy

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

August Krogh Building

Copenhagen, Denmark

Location

Related Publications (1)

  • Kissow J, Jacobsen KJ, Jessen S, Thomsen LB, Quesada JP, Bangsbo J, Deshmukh AS, Hostrup M. Global Proteomics Reveals Distinct Muscle Adaptations to Menstrual Cycle Phase-Based Sprint Interval Training in Endurance-Trained Females. Mol Cell Proteomics. 2025 Oct;24(10):101053. doi: 10.1016/j.mcpro.2025.101053. Epub 2025 Aug 14.

    PMID: 40818533DERIVED

MeSH Terms

Interventions

Endurance TrainingResistance TrainingHigh-Intensity Interval Training

Intervention Hierarchy (Ancestors)

Exercise TherapyRehabilitationAftercareContinuity of Patient CarePatient CareTherapeuticsPhysical Therapy ModalitiesPhysical Conditioning, HumanExerciseMotor ActivityMovementMusculoskeletal Physiological PhenomenaMusculoskeletal and Neural Physiological Phenomena

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Associate Professor

Study Record Dates

First Submitted

October 21, 2019

First Posted

October 23, 2019

Study Start

October 30, 2019

Primary Completion

November 28, 2023

Study Completion

November 28, 2023

Last Updated

December 27, 2023

Record last verified: 2023-12

Locations

DK(1)