Effect of an Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

NCT04156113 | Completed

• 1 other identifier: 18-10.2/56
• Study Type: interventional
• Target: 86
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect. Therefore, this study hypothesizes that: H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise. H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism. H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels. H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism. H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.

Conditions

Uncoupling Protein-1; Polymorphism, Restriction Fragment Length; Irisin

Keywords

Beige fat cell; Fat tissue; Athletes; Sedentary people; Irisin

Outcome Measures

Primary Outcomes (3)

• Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

  Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory.

  15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

• Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

  Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory.

  15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

• Uncoupling protein-1-3826 A/G polymorphism determination

  Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory.

  15 minutes before the Yo-Yo intermittent recovery test (level 1)

Secondary Outcomes (2)

• Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

  15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

• Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

  15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

Other Outcomes (15)

• Change from the baseline serum C reactive protein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

  15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

• Change from the baseline serum creatine kinase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

  15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

• Change from the baseline serum alanine aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

  15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

Study Arms (2)

Athletes Group

EXPERIMENTAL

The athletes group will be composed of healthy, non obese (body mass index \< 30), male basketball, volleyball and handball players aged between 18 and 35 years who have been training regularly for at least 3 months. Participants must not be regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Intervention: Yo-Yo intermittent recovery test (level 1) is administered.

Other: Yo-Yo intermittent recovery test (level 1)

Sedentary Group

EXPERIMENTAL

The sedentary group will be composed of healthy, non obese (body mass index \< 30), male aged between 18 and 35 years who have not been training regularly for at least 3 months. Participants must not be regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Intervention: Yo-Yo intermittent recovery test (level 1) is administered.

Other: Yo-Yo intermittent recovery test (level 1)

Interventions

Yo-Yo intermittent recovery test (level 1) OTHER

The Yo-Yo intermittent recovery test (level 1) will be administered once.

Athletes Group; Sedentary Group

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Athletes group:
• Must be basketball, or volleyball, or handball players.
• Must be subjects who have been training regularly for at least 3 months.
• Sedentary group:
• \* Must be subjects who have not been training regularly for at least 3 months.
• For both groups:
• Healthy.
• Non obese (body mass index \< 30).
• Aged between 18 and 35 years.

You may not qualify if:

• For both groups:
• Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances.
• Being obese (body mass index \> 30).
• Having an acute or chronic injury which is limiting physical performance.
• Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples.
• Being injured or sick during the study.

Sponsors & Collaborators

• Eastern Mediterranean University: lead
• Ege University: collaborator

Study Sites (1)

Ege University Faculty of Sport Sciences

Izmir, Turkey (Türkiye)

Location

Study Officials

• Faruk Turgay, Assoc. Prof

  Ege University, Faculty of Sports Science

  STUDY CHAIR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Senior instructor

Study Record Dates

• First Submitted: November 4, 2019
• First Posted: November 7, 2019
• Study Start: November 11, 2019
• Primary Completion: September 2, 2020
• Study Completion: September 7, 2020
• Last Updated: September 9, 2020

Record last verified: 2020-09

Locations

TU (1)