Vitamin D Receptor, Interleukin-1A and Interleukin-1B Gene Polymorphisms in Periodontitis

NCT04806971 | Completed

• 1 other identifier: SAG-K-170118-0002
• Study Type: observational
• Target: 200
• Locations: 1 country
• Sites: 2

Brief Summary

Background: The aim of the study is to find out the prevelance of VDR (rs731236), IL1B (rs1143634) and IL1A (rs1800587)gene polymorphisms in Turkish population and their association with stage III grade B / C periodontitis. Methods: Individuals who were found to be systemically and periodontally healthy (N = 100) after clinical and radiographic examination and who were diagnosed with Stage III Grade B / C periodontitis (N = 100) were included in the study. Gingival index, Plaque index, bleeding on probing, clinical attachment level and pocket depths of the patients were recorded. subjects genotyped for rs731236, rs1143634 and rs1800587 gene polymorphisms with Real Time Polymerase Chain Reaction.

Conditions

Periodontitis; Polymorphism, Restriction Fragment Length

Keywords

Vitamin D receptor; Polymorphism; Periodontitis,; Interleukin-1B; Interleukin-1A

Outcome Measures

Primary Outcomes (3)

• IL-1A gene polymorphism

  baseline

• Interleukin-1B gene polymorphism

  baseline

• Vitamin D Receptor gene polymorphism

  baseline

Study Arms (2)

Periodontitis

Patients with grade B / C, stage III periodontitis were classified based on the new classification criteria.Clinical attachment loss ≥5 mm, probing depth (PD) ≥6 mm, ≥20 teeth and radiographic bone loss extending to the root middle third. Grade was assessed considering the radiographic bone loss in the most affected tooth in the dentition as a function of age (Grade B=0.25-1.0, Grade C= \>1.0).

Healthy

The periodontally healthy subjects without any clinical sign of inflammation,not showing a history of periodontitis; PD ≤3 mm; \<10% of sites with BOP; an absence of detectable bone loss and/or attachment ; without extensive caries or restorations and presence of at least 28 permanent teeth. In addition, all the control subjects showed the absence of any local or systemic pathology.

Eligibility Criteria

• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

200 subjects were recruited from the Marmara University, Faculty of Dentistry, Clinics of Periodontology

You may qualify if:

• systemically healthy, previously untreated periodontitis patients

You may not qualify if:

• presence of systemic disease such as diabetes, rheumatic fever, liver or kidney diseases, neurologic deficiencies, immunologic diseases; pregnancy; smoking; receive of any medication which may affect periodontal tissues; used antibiotics for any purpose within 6 months.

Sponsors & Collaborators

• Marmara University: lead

Study Sites (2)

Marmara University

Istanbul, Maltepe, 34854, Turkey (Türkiye)

Location

Marmara University Dental Faculty Periodontology Department

Istanbul, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Periodontitis

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 16, 2021
• First Posted: March 19, 2021
• Study Start: January 29, 2018
• Primary Completion: September 27, 2019
• Study Completion: September 27, 2019
• Last Updated: December 15, 2021

Record last verified: 2021-11

Locations

TU (2)