The Effect of Maximal Exercise on MMP9 and TIMP1 and The Role of MMP9 -1562 C/T and TIMP1 372 T/C Polymorphisms

NCT04157556 | Completed

• 1 other identifier: 18-7.1/49
• Study Type: interventional
• Target: 86
• Locations: 1 country
• Sites: 1

Brief Summary

Matrix metalloproteinases (MMPs) play a critical role in the remodeling and regeneration of skeletal muscle. Studies which investigate exercise and effects of MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs) are contradictory. Also the role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on exercise are unclear. In accordance with this conditions the hypothesis of the study are as follows:

1. 1.Levels of basal serum MMP9 and TIMP1 are different between athletes (volleyball, basketball, handball) and sedentary people.
2. 2.Levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people .
3. 3.Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people.
4. 4.Levels of basal serum MMP9 and TIMP1 are related to MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms.
5. 5.Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise and MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms are different between athletes (volleyball, basketball, handball) and sedentary people.

Conditions

MMP9; TIMP1; MMP9 -1562 C/T; TIMP1 372 T/C

Outcome Measures

Primary Outcomes (2)

• Level of MMP9 and TIMP1

  The effect of acute maximal exercise on serum MMP9 and TIMP1 levels in athletes and sedentary people.

  Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)

• MMP9 -1562 C/T, TIMP1 372 T/C polymorphisms

  The role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on the effect of acute maximal exercise on serum MMP9 and TIMP1 in athletes and sedentary people.

  Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)

Secondary Outcomes (2)

• Level of indicators of muscle damage and inflammation and oxidative stress

  Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)

• Level of blood lipids and lipoproteins, glucose, urea, creatinine, hemogram

  Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)

Study Arms (2)

Group of athletes

EXPERIMENTAL

Age: 18-35 Gender: Male Basketball, volleyball, handball players who have been training regularly for at least last 3 months.

Other: Yo-Yo Intermittent Recovery Test Level 1

Group of sedentary people

EXPERIMENTAL

Age: 18-35 Gender: Male Individuals with similar physical characteristics to the group of athletes and who have not exercise regularly for at least last 3 months.

Other: Yo-Yo Intermittent Recovery Test Level 1

Interventions

Yo-Yo Intermittent Recovery Test Level 1 OTHER

The test is a standard test battery with progressively increasing speeds. After every 40-m run (2x20 m), active rest is performed by walking or jogging at a distance of 10 m (2x5 m). for 10 seconds. Participants are trying to adjust their speed according to incoming audio signals.The test is terminated when the participants can not reach the signal twice. The test ends in 10-20 minutes depending on the level of participants. Reference Bangsbo, J., Iaia, F. M., \& Krustrup, P. (2008). The Yo-Yo intermittent recovery test. Sports medicine, 38(1), 37-51. Krustrup, P., Mohr, M., Amstrup, T., Rysgaard, T., Johansen, J., Steensberg, A., ... \& Bangsbo, J. (2003). The yo-yo intermittent recovery test: physiological response, reliability, and validity. Medicine \& Science in Sports \& Exercise, 35(4), 697-705.

Group of athletes; Group of sedentary people

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Group of athletes:
• Male basketball players who have been training regularly for at least last 3 months.
• Male volleyball players who have been training regularly for at least last 3 months.
• Male handball players who have been training regularly for at least last 3 months.
• Group of sedentary people:
• Male individuals with similar physical characteristics to the group of athletes
• Male individuals who have not exercise regularly for at least last 3 months.

You may not qualify if:

• Smoking regularly
• Drinking alcohol regularly
• Using drug and antioxidant substance
• Being obese,
• Being sick or having disability

Sponsors & Collaborators

• Dokuz Eylul University: lead
• Ege University: collaborator

Study Sites (1)

Ege University Faculty of Sports Science

Izmir, Turkey (Türkiye)

Location

Study Officials

• Faruk Turgay, associate professor

  Ege University, Faculty of Sports Science

  STUDY CHAIR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: research assistant

Model Details: There are 2 groups in the study: Athletes and sedentary people.They will apply the same acute exercise protocol (The Yo-Yo Intermittent Recovery Test Level 1). A blood test will conduct before and after the acute exercise protocol.

Study Record Dates

• First Submitted: November 5, 2019
• First Posted: November 8, 2019
• Study Start: November 11, 2019
• Primary Completion: January 3, 2020
• Study Completion: April 28, 2020
• Last Updated: April 30, 2020

Record last verified: 2020-04

Locations

TU (1)