The Acute Effect of Different Resistance Exercise Loads on Irisin and Brain-derived Neurotrophic Factor in Blood Serum and Plasma

NCT07189130 | Active Not Recruiting

• 1 other identifier: 0120-222/2024-2711-3
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.

Conditions

BDNF; Irisin; Resistance Exercise; Stroop Test; Lactate; Healthy Young Adult

Outcome Measures

Primary Outcomes (5)

• plasma BDNF

  BDNF will be determined in blood plasma with ELISA kit.

  pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

• Irisin

  Irisin will be measured in blood plasma.

  pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

• serum BDNF

  BDNF will be determined in blood serum with ELISA kit.

  pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

• Platelet count

  Platelet count in blood will be done.

  pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

• BDNF per platelet

  The amount of BDNF per platelet will be calculated.

  pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

Secondary Outcomes (2)

• Lactate

  pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

• Stroop test

  pre, 15 minutes post exercise.

Study Arms (1)

Intervention: participants going through exercising or control

EXPERIMENTAL

All participants will do 1 control protocol and 3 resistance exercises protocols. Order of protocols will be randomized.

Other: Exercise: 80% 1 RM with 2 minutes break; Other: Exercise: 80% 1RM with1 minute break; Other: Exercise: accenuated eccentric exercise; Other: Control

Interventions

Exercise: 80% 1 RM with 2 minutes break OTHER

Participant will do resistance exercise at 80% 1RM with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.

Also known as: resistance exercise

Intervention: participants going through exercising or control

Exercise: 80% 1RM with1 minute break OTHER

Participant will do resistance exercise at 80% 1RM with 1 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.

Also known as: resistance exercise

Intervention: participants going through exercising or control

Exercise: accenuated eccentric exercise OTHER

Participant will do accenuated eccentric resistance exercise at 80% 1RM in concetric part of movement and 110% 1RM in eccentric part of movement with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.

Intervention: participants going through exercising or control

Control OTHER

Timeline of events will be same as in exercise protocol (blood withdrawal and Stroop test), except participants will not do any kind of exercise protocol, instead they will be seating.

Intervention: participants going through exercising or control

Eligibility Criteria

• Age: 18 Years - 30 Years
• Gender Eligibility Details: Biological males.
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• males between 18 and 30 years
• moderate level of physical activity assessed with IPAQ

You may not qualify if:

• injuries
• cardiovascular diseases
• respiratory diseases
• psychiatric diseases
• metabolic diseases
• other neurodegenerative diseases

Sponsors & Collaborators

• University of Ljubljana: lead

Study Sites (1)

University of Ljubljana, Faculty of Sport

Ljubljana, 1000, Slovenia

Location

MeSH Terms

Interventions

Exercise; Resistance Training

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: August 19, 2025
• First Posted: September 23, 2025
• Study Start: May 12, 2025
• Primary Completion: November 25, 2025
• Study Completion (Estimated): September 1, 2026
• Last Updated: December 16, 2025

Record last verified: 2025-12

Locations

SL (1)