The Effects of Chronic Exercise on UCP1 Gene in Human White Adipose Tissue

NCT04039685 | Completed

• 1 other identifier: 4. Browning of WAT
• Study Type: interventional
• Target: 32
• Locations: 1 country
• Sites: 1

Brief Summary

Obesity is characterized by excessive white adipose tissue (WAT) that increases risk for disease. About 700 million adults are obese worldwide, designating the need of reducing excessive WAT in humans and subsequently the risk for disease. For this reason, previous research focused on the thermogenic capacity of white adipocytes in response to chronic exercise. It is suggested that the latter occurs due to increased demand of oxidation during exercise to maintain non-esterified fatty acids flux in the circulation, thus to maintain energy supply White adipocytes express the uncoupling protein one (UCP1), which dissipates chemical energy in the mitochondria to produce heat in response to cold exposure. UCP1 was thought that exclusively appears in brown adipose tissue (BAT), which during the mitochondrial electron transport chain process, bypasses the adenosine triphosphate (ATP) synthase to produce heat, as a thermoregulatory mechanism of maintaining body temperature. Its presence in white adipocytes, indicates a thermogenic capacity of WAT, which could lead to similar health benefits as those reported for increased BAT activity; mainly reduced body mass index (BMI) and fat mass, which designate body weight loss and reduced obesity phenotype. The investigators aimed to identify whether UCP1 in human WAT is altered by chronic exercise.

Conditions

Obese; Obesity

Keywords

uncoupling protein 1; uncoupling protein one; resistance exercise; aerobic exercise; browning formation; white fat

Outcome Measures

Primary Outcomes (2)

• UCP1 mRNA expression in white adipocytes

  UCP1 mRNA expression was measured via fat biopsy samples from the subcutaneous abdominal area at baseline, at the end of the exercise intervention (8th week) and at the end of the de-training period (16th week)

  Baseline measurements; measurements at the end of the exercise intervention (8th week); measurements at the end of the de-training period (16th week)

• UCP1 protein concentration in white adipocytes

  UCP1 protein concentration was measured via fat biopsy samples from the subcutaneous abdominal area at baseline, at the end of the exercise intervention (8th week) and at the end of the de-training period (16th week)

  Baseline measurements; measurements at the end of the exercise intervention (8th week); measurements at the end of the de-training period (16th week)

Study Arms (4)

Aerobic exercise group

EXPERIMENTAL

Other: Effects of an eight weeks aerobic exercise followed by an eight weeks de-training period on UCP1 gene of human white adipose tissue

Resistance exercise group

EXPERIMENTAL

Other: Effects of an eight weeks resistance exercise followed by an eight weeks de-training period on UCP1 gene of human white adipose tissue

Combined (aerobic+resistance) exercise group

EXPERIMENTAL

Other: Effects of an eight weeks combined (aerobic+resistance) exercise followed by an eight weeks de-training period on UCP1 gene of human white adipose tissue

Non-exercise group

NO INTERVENTION

Interventions

Effects of an eight weeks aerobic exercise followed by an eight weeks de-training period on UCP1 gene of human white adipose tissue OTHER

The investigators evaluated the effects of an eight weeks aerobic exercise program and a following of eight weeks of a de-training period on UCP1 gene in previously non-regular exercisers healthy adult men

Aerobic exercise group

Effects of an eight weeks resistance exercise followed by an eight weeks de-training period on UCP1 gene of human white adipose tissue OTHER

The investigators evaluated the effects of an eight weeks resistance exercise program and a following of eight weeks of a de-training period on UCP1 gene in previously non-regular exercisers healthy adult men

Resistance exercise group

Effects of an eight weeks combined (aerobic+resistance) exercise followed by an eight weeks de-training period on UCP1 gene of human white adipose tissue OTHER

The investigators evaluated the effects of an eight weeks combined (aerobic+resistance) exercise program and a following of eight weeks of a de-training period on UCP1 gene in previously non-regular exercisers healthy adult men

Combined (aerobic+resistance) exercise group

Eligibility Criteria

• Age: 18 Years - 55 Years
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• adult men
• healthy
• no chronic disease
• no medication treatment
• no regular exercisers

You may not qualify if:

• women
• non-adult men
• chronic disease individuals
• any medication treatment
• acute illness
• regular exercisers

Sponsors & Collaborators

• Petros Dinas: lead

Study Sites (1)

Petros Dinas

Trikala, Thessaly, 42100, Greece

Location

MeSH Terms

Conditions

Obesity

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: SCREENING
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Senior Researcher in human physiology

Study Record Dates

• First Submitted: July 26, 2019
• First Posted: July 31, 2019
• Study Start: June 1, 2013
• Primary Completion: May 30, 2014
• Study Completion: June 30, 2014
• Last Updated: July 31, 2019

Record last verified: 2019-07

Locations

GR (1)