NCT04016337

Brief Summary

During last decades, an excessive intake of sugars has been observed in westernized countries, mainly due to the consumption of sugar-rich soft drinks. Epidemiological studies have pointed out a positive correlation between the consumption of such drinks and the risk of obesity, diabetes, and cardiovascular diseases. Therefore, there is a growing trend looking for new non-caloric alternatives (sweeteners) in order to reduce the sugar content of foodstuffs without losing their sweetness and tastefulness. However, some questions regarding sweeteners have recently arisen as their effects after long-term consumption have not been fully unravelled. They could also contribute to a higher caloric intake and to disrupt the gut microbiota. Besides seeking new alternatives for the high intake of sugar-rich drinks, there is a need for increasing the consumption of fruits and vegetables among general public. Developing new products to stretch the shelf-life of fresh fruits, preserving their nutrients, and reducing the energetic content of fruit juices should also be accomplished. In this sense, lemon juice has been proposed as an interesting ingredient in the elaboration of soft drinks due to its distinctive aroma and high nutritive value. Its combination with maqui berry juices contributes to stabilize the colour and phenolic compounds that both products contain (mainly flavanones for lemon juice and anthocyanins for maqui). The beneficial effects of these vegetal foodstuffs and their phenolic substances on glucose intolerance have been reported.The aim of BEBESANO is studying the beneficial effects of sugar-free, rich-in-phytochemicals drink consumption on postprandial hyperglycaemia, in order to provide alternatives to excessive sugar intake and counteract the postprandial response linked to sugar consumption in subjects with low levels of chronic inflammation such as overweight people. Moreover, BEBESANO will shed light on the interactions of food constituents with the main pathways involved in the lipid and glucose metabolism, oxidative stress, inflammation, and hormonal regulation in overweight population.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
138

participants targeted

Target at P50-P75 for not_applicable obesity

Timeline
Completed

Started Sep 2017

Shorter than P25 for not_applicable obesity

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

September 1, 2017

Completed
7 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 30, 2018

Completed
5 months until next milestone

Study Completion

Last participant's last visit for all outcomes

August 31, 2018

Completed
10 months until next milestone

First Submitted

Initial submission to the registry

July 2, 2019

Completed
9 days until next milestone

First Posted

Study publicly available on registry

July 11, 2019

Completed
Last Updated

July 19, 2019

Status Verified

July 1, 2019

Enrollment Period

7 months

First QC Date

July 2, 2019

Last Update Submit

July 17, 2019

Conditions

Keywords

sweetenerssteviasucralosepolyphenolsmaquibioavailabilityinflammationgut hormonesbiomarkers

Outcome Measures

Primary Outcomes (7)

  • Changes in markers of inflammation (cytokines)

    Plasma levels of IL-6, TNF-alfa, IL-10

    Changes observed from baseline at day 0 compared to 60 days

  • Changes in markers of inflammation (c-reactive protein)

    Plasma levels of c-reactive protein (mg/dL)

    Changes observed from baseline at day 0 compared to 60 days

  • Changes in markers of lipid metabolism

    Plasma concentrations of Total cholesterol, LDL, HDL, Triglycerides

    Changes observed from baseline at day 0 compared to 60 days

  • Changes in markers of glycemic metabolism

    Plasma levels of insulin and glucose

    Changes observed from baseline at day 0 compared to 60 days

  • Insulin resistance

    Insulin and glucose levels will be combined to report HOMA index

    Changes observed from baseline at day 0 compared to 60 days

  • Changes in hormone response

    Plasma levels of leptin, ghrelin

    Changes observed from baseline at day 0 compared to 60 days

  • Changes in anthropometric parameters

    weight and height will be combined to report BMI in kg/m\^2

    Changes observed from baseline at day 0 compared to 60 days

Study Arms (3)

Intervention with beverage added with sucralose

EXPERIMENTAL

A beverage (330 mL) made with lemon and maqui extracts and sweetened with sucralose was taken by participants, during 60 days. Beverages were made in a semi-industrial process in a pilot plant. Microbiological tests, quality controls and shelf-life assays were performed following legislation on beverages. Concentrations of lemon and maqui extract are under industrial secret. The sweetener sucralose was added within the ranges established by law as maximums. The beverage was carried out in the pilot plant of a company (Nutracitrus) with all the safety systems, tested by an external lab (Ecosur), apart from the routine controls themselves. All ingredients were suitable for consumption and purchased from different companies with their respective certificates and conservation was at 5 º C for a maximum of 7 days, prior to administration to volunteers.

Dietary Supplement: Beverages

Intervention with beverage added with saccharose

EXPERIMENTAL

A beverage (330 mL) made with lemon and maqui extracts and sweetened with saccharose was taken by participants, during 60 days. Beverages were made in a semi-industrial process in a pilot plant. Microbiological tests, quality controls and shelf-life assays were performed following legislation on beverages. Concentrations of lemon and maqui extract are under industrial secret. The sweetener saccharose was added within the ranges established by law as maximums. The beverage was carried out in the pilot plant of a company (Nutracitrus) with all the safety systems, tested by an external lab (Ecosur), apart from the routine controls themselves. All ingredients were suitable for consumption and purchased from different companies with their respective certificates and conservation was at 5 º C for a maximum of 7 days, prior to administration to volunteers.

Dietary Supplement: Beverages

Intervention with beverage added with stevia

EXPERIMENTAL

A beverage (330 mL) made with lemon and maqui extracts and sweetened with stevia extract was taken by participants, during 60 days. Beverages were made in a semi-industrial process in a pilot plant. Microbiological tests, quality controls and shelf-life assays were performed following legislation on beverages. Concentrations of lemon and maqui extract are under industrial secret. The sweetener stevia was added within the ranges established by law as maximums. The beverage was carried out in the pilot plant of a company (Nutracitrus) with all the safety systems, tested by an external lab (Ecosur), apart from the routine controls themselves. All ingredients were suitable for consumption and purchased from different companies with their respective certificates and conservation was at 5 º C for a maximum of 7 days, prior to administration to volunteers.

Dietary Supplement: Beverages

Interventions

BeveragesDIETARY_SUPPLEMENT

The beverages were all made with lemon and maqui extracts, and different sweeteners

Intervention with beverage added with saccharoseIntervention with beverage added with steviaIntervention with beverage added with sucralose

Eligibility Criteria

Age35 Years - 55 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • healthy volunteers,
  • body mass index (BMI) between 25 - 29.9 kg/m2 (overweight according to WHO criteria)
  • years,
  • absence of disease.

You may not qualify if:

  • Hypertension
  • Dislipemia
  • Smoking

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Universidad Católica de Murcia (UCAM)

Guadalupe, Murcia, 30107, Spain

Location

Related Publications (1)

  • Villano D, Zafrilla P, Garcia-Viguera C, Dominguez-Perles R. A UHPLC/MS/MS method for the analysis of active and inactive forms of GLP-1 and GIP incretins in human plasma. Talanta. 2022 Jan 1;236:122806. doi: 10.1016/j.talanta.2021.122806. Epub 2021 Aug 19.

MeSH Terms

Conditions

ObesityInflammationInsulin Resistance

Interventions

Beverages

Condition Hierarchy (Ancestors)

OverweightOvernutritionNutrition DisordersNutritional and Metabolic DiseasesBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsPathologic ProcessesHyperinsulinismGlucose Metabolism DisordersMetabolic Diseases

Intervention Hierarchy (Ancestors)

Diet, Food, and NutritionPhysiological PhenomenaFood and Beverages

Study Officials

  • Débora Villaño Valencia, PhD

    Universidad Católica de Murcia (UCAM)

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Masking Details
Beverages were provided by manufacturer with codes and no one, up to now, knows the sweetener added on each type.
Purpose
PREVENTION
Intervention Model
PARALLEL
Model Details: The chronic study consisted on a 60 days consumption of a beverage (330 mL) made with lemon and maqui and added a particular sweetener.
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Professor

Study Record Dates

First Submitted

July 2, 2019

First Posted

July 11, 2019

Study Start

September 1, 2017

Primary Completion

March 30, 2018

Study Completion

August 31, 2018

Last Updated

July 19, 2019

Record last verified: 2019-07

Data Sharing

IPD Sharing
Will not share

Locations