Examining the Relationship Between Acute Meal Intake and Inflammation in Children

NCT03597542 | Completed

• 1 other identifier: 17112
• Study Type: interventional
• Target: 36
• Locations: 1 country
• Sites: 1

Brief Summary

Recent evidence has shown that obese and overweight children exhibit states of chronic inflammation. In obese adults, a high carbohydrate meal induces an inflammatory response; however, the effects of a high carbohydrate meal on biomarkers of inflammation has not previously been examined in children. The purpose of this research project is to characterize the inflammatory response to a high carbohydrate versus a low carbohydrate meal in healthy weight, overweight, and obese children (age 7-17 years). After completing informed consent/assent, a Dual-energy X-ray Absorptiometry (DXA) scan, and baseline blood draw on experimental day 1, children will then return to the lab two times to ingest either a high or low carbohydrate beverage followed by post-meal blood collection for a total of 3 blood draws. Biomarkers of inflammation will be analyzed by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Participants will be recruited through various studies through the Neurocognitive Kinesiology Lab/Body Composition and Nutritional Neuroscience Labs as well as through the local Champaign-Urbana community. Data from this project will provide a better understanding of the inflammatory response to different meals in healthy weight, overweight, and obese children.

Conditions

Obesity; Inflammation

Keywords

Children; Inflammation; Obesity

Outcome Measures

Primary Outcomes (1)

• Effects of egg or maltodextrin on inflammatory cytokines (Tumor necrosis factor-alpha, C-reactive protein, and Interleukin-6)

  Will be used by determining any changes in inflammatory cytokines via enzyme-linked immunosorbent assay (ELISA) kits.

  3 weeks.

Secondary Outcomes (1)

• Effects of egg or maltodextrin on inflammatory monocyte cells with and without expression of Toll-like receptor 4 and C-C Chemokine receptor-2.

  3 weeks.

Study Arms (2)

Maltodextrin

EXPERIMENTAL

Participants will consume 56g of maltodextrin dissolved in 500mL of water.

Other: Eggs and Carbohydrates

Egg

EXPERIMENTAL

Participants will consume 36g of spray-dried egg powder (equivalent to 3 eggs) dissolved in 500mL of water.

Other: Eggs and Carbohydrates

Interventions

Eggs and Carbohydrates OTHER

Participants are asked to consume both egg based powder or maltodextrin dissolved in 500mL of water at two different time points at least one week apart. Participants will then submit to a venous blood draw to assess levels of cellular inflammation.

Egg; Maltodextrin

Eligibility Criteria

• Age: 7 Years - 17 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)

You may qualify if:

• Between the ages of 7-17

You may not qualify if:

• Younger than 7 years and older than 17 years
• Presence of allergies to eggs and/or sugar substitutes
• Presence of heart or respiratory disease
• Presence of uncontrolled hypertension, diabetes
• Use of anti-inflammatory medications
• Presence of cancer or metabolic disease

Sponsors & Collaborators

• University of Illinois at Urbana-Champaign: lead
• Egg Nutrition Center: collaborator

Study Sites (1)

University of Illinois at Urbana-Champaign

Urbana, Illinois, 61801, United States

Location

Related Publications (1)

• Niemiro GM, Chiarlitti NA, Khan NA, De Lisio M. A Carbohydrate Beverage Reduces Monocytes Expressing TLR4 in Children with Overweight or Obesity. J Nutr. 2020 Mar 1;150(3):616-622. doi: 10.1093/jn/nxz294.

  PMID: 31825075 DERIVED

MeSH Terms

Conditions

Obesity; Inflammation

Interventions

Eggs; Carbohydrates

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Pathologic Processes

Intervention Hierarchy (Ancestors)

Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Masking Details: Both the participants and researchers involved with data analysis will be blinded to which condition given.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Model Details: Double blind randomized cross-over design

Study Record Dates

• First Submitted: July 13, 2018
• First Posted: July 24, 2018
• Study Start: August 29, 2017
• Primary Completion: February 2, 2018
• Study Completion: February 2, 2018
• Last Updated: July 31, 2018

Record last verified: 2018-07

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)