Comparing Acute Aerobic and Resistance Exercise

NCT03794050 | Terminated

• 1 other identifier: STUDY00001191
• Study Type: interventional
• Target: 35
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to determine the effect of age and fitness level on the physiological response to an acute bout of resistance or aerobic exercise.

Conditions

Exercise Physiology

Outcome Measures

Primary Outcomes (15)

• Monocyte subset and function

  Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function

  Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise

• Monocyte subset and function

  Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function

  Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise

• Monocyte subset and function

  Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function

  compare pre-RT to pre-AT

• Monocyte subset and function

  Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function

  compare immediately post-RT to immediately post-AT

• Monocyte subset and function

  Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function

  compare 1hr post-RT to 1hr post-AT

• NK cell function

  Flow cytometry to identify NK cells and in vitro activity to assess NK activity

  Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise

• NK cell function

  Flow cytometry to identify NK cells and in vitro activity to assess NK activity

  Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise

• NK cell function

  Flow cytometry to identify NK cells and in vitro activity to assess NK activity

  compare pre-RT to pre-AT

• NK cell function

  Flow cytometry to identify NK cells and in vitro activity to assess NK activity

  compare immediately post-RT to immediately post-AT

• NK cell function

  Flow cytometry to identify NK cells and in vitro activity to assess NK activity

  compare 1hr post-RT to 1hr post-AT

• Circulating relative and absolute counts of T cell subsets

  Flow cytometry to identify T cell subsets

  Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise

• Circulating relative and absolute counts of T cell subsets

  Flow cytometry to identify T cell subsets

  Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise

• Circulating relative and absolute counts of T cell subsets

  Flow cytometry to identify T cell subsets

  compare pre-RT to pre-AT

• Circulating relative and absolute counts of T cell subsets

  Flow cytometry to identify T cell subsets

  compare immediately post-RT to immediately post-AT

• Circulating relative and absolute counts of T cell subsets

  Flow cytometry to identify T cell subsets

  compare 1hr post-RT to 1hr post-AT

Secondary Outcomes (5)

• Serum cortisol

  Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise

• Serum cortisol

  Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise

• Serum cortisol

  compare pre-RT to pre-AT

• Serum cortisol

  compare immediately post-RT to immediately post-AT

• Serum cortisol

  compare 1hr post-RT to 1hr post-AT

Study Arms (2)

resistance training exercise

EXPERIMENTAL

All participants complete one session of resistance training exercise

Other: Resistance training exercise

aerobic training exercise

EXPERIMENTAL

All participants complete one session of aerobic training exercise

Other: Aerobic training exercise

Interventions

Resistance training exercise OTHER

randomized complete crossover for acute resistance training exercise session

resistance training exercise

Aerobic training exercise OTHER

randomized complete crossover for acute aerobic exercise training exercise session

aerobic training exercise

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• "Young" Adults: ages 18-30
• "Older" Adults: ages 55-75 and if a female postmenopausal
• Participants classified as "trained" must participate in at least 30 minutes of moderate to vigorous intensity cardiovascular activity at least 3 days per week and engage in resistance training for all of the major muscle groups approximately 2 days / week. They must have upheld this activity for the past 3 months
• Participants classified as "untrained" must be participating in less than 30 minutes of moderate to vigorous intensity cardiovascular activity and less than 2 days of resistance training per week for at least the last 3 months.

You may not qualify if:

• Have any contraindications to moderate to vigorous exercise
• Range of motion restrictions that would prevent them from participating in aerobic or resistance training with proper form (they must be ambulatory)
• aking medications (prescription or over the counter) known to influence immune function, including daily NSAID's, beta blockers, statins, bisphosphonates, or steroids.
• Have known cardiovascular, respiratory, metabolic, or renal disease, with the exception of controlled hypertension (as defined by resting BP below 140/90) and/or controlled asthma (self-reported).
• Be pregnant
• Be unable to complete all visits (i.e. must not be planning to leave the Houston area long-term before concluding their participation in the study which will span several weeks)
• Fall outside of a BMI range of (18.5 - 30)
• Consume alcohol or recreational drugs for 24h prior to visits
• Older women cannot be pre-menopausal (must have not had a menses for at least 12 months)
• Unable to speak or read English
• Participants must not have scheduling conflicts that would prevent them from reporting to the laboratory of integrated physiology 4 times over the course of the study

Sponsors & Collaborators

• University of Houston: lead

Study Sites (1)

University of Houston

Houston, Texas, 77204, United States

Location

Related Publications (1)

• Graff RM, Jennings K, LaVoy ECP, Warren VE, Macdonald BW, Park Y, Markofski MM. T-cell counts in response to acute cardiorespiratory or resistance exercise in physically active or physically inactive older adults: a randomized crossover study. J Appl Physiol (1985). 2022 Jul 1;133(1):119-129. doi: 10.1152/japplphysiol.00301.2021. Epub 2022 May 26.

  PMID: 35616304 DERIVED

MeSH Terms

Interventions

Resistance Training

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Melissa Markofski, PhD

  University of Houston

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: SUPPORTIVE CARE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: December 17, 2018
• First Posted: January 4, 2019
• Study Start: December 18, 2018
• Primary Completion: March 31, 2020
• Study Completion: March 31, 2020
• Last Updated: March 24, 2023

Record last verified: 2023-03

Locations

US (1)