Singapore PREconception Study of Long-Term Maternal and Child Outcomes

NCT03531658 | Active Not Recruiting

• 3 other identifiers: 2019/2143/DNMRC/TCR/012-NUHS/2014MOH-000504
• Study Type: observational
• Target: 1,054
• Locations: 1 country
• Sites: 1

Brief Summary

We aim to test the following primary hypothesis that nutrition, lifestyle, and maternal emotional health prior to pregnancy and/or during the first trimester alter the expression of metabolic or neurodevelopmental endophenotypes with accompanying effects on the epigenome/transcriptome of the offspring.

Conditions

Metabolic Disease; Neurological and Mental Health Conditions; Genetics Predisposition; Skin Condition; Environmental Exposure; Socioeconomic Factors; Psychology, Social

Keywords

Cohort Study; Birth Cohort; Longitudinal Study; Pregnancy; Growth; Developmental Origins of Health and Disease; DOHaD; Epidemiology; Maternal Health; Infant Health; Child Health; Health Outcomes; Postnatal Exposure; Perinatal Exposure; Environmental Factors; Nutrition; Genetics

Outcome Measures

Primary Outcomes (2)

• Offspring adiposity

  Offspring adiposity (total and percentage fat mass) measured by skinfold calipers, air-displacement plethysmography, quantitative magnetic resonance (QMR), Bioelectrical Impedance Analysis (BIA), and Magnetic resonance imaging (MRI).

  From birth until 9 years of age

• Offspring neurocognitive and behavioural development

  Offspring neurocognitive and behavioural development measured by questionnaires, behavioural tasks and brain MRI.

  From 3-12 months up till 9 years of age

Secondary Outcomes (39)

• Duration of gestation

  Up to 42 weeks, between estimated date of conception based on ultrasound scan and date of delivery

• Fetal growth measured by ultrasound scan

  Up to 30 weeks, between 6-36 weeks of gestation

• Offspring body weight measured by calibrated weighing scale

  From birth until 9 years of age

• Offspring pubertal assessment

  From 7 until 9 years of age

• Offspring growth trajectories

  From birth until 9 years of age

Other Outcomes (2)

• Offspring health obtained from questionnaires and clinical documents

  From birth until 9 years of age

• Maternal health obtained from questionnaires and clinical documents

  Between preconception, pregnancy and 9 years postpartum

Eligibility Criteria

• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Healthy women aged between 18 and 35 who are actively planning to conceive within a 1-year time frame upon recruitment, and later along with their children born from the indexed pregnancy.

You may qualify if:

• Women aged 18-35 years
• Intention to reside in Singapore for the next 5 years
• Chinese, Malay, Indian or any combinations of these 3 ethnic groups
• Planning to conceive within 1 year
• Able to provide written, informed consent

You may not qualify if:

• Have been actively trying to conceive for more than 18 months
• Currently pregnant
• Assisted fertility, apart from those taking clomiphene alone in the past 1 month
• Established pre-existing diabetes (type 1 or type 2)
• Oral or implanted contraception, or with an IUCD in situ in the past 1 month
• On systemic steroids in the past 1 month
• On anticonvulsants in the past 1 month
• On HIV or Hepatitis B or C medication in the past 1 month
• Not becoming pregnant after more than 12 months from recruitment
• Eventual multiple pregnancies
• Pregnancy complications such as miscarriages, ectopic pregnancy, still birth
• The partners of the women will also be invited to participate in the study. Babies born from these mothers will be followed up until the child is 9 years of age.

Sponsors & Collaborators

• KK Women's and Children's Hospital: lead
• National University Health System, Singapore: collaborator
• Institute for Human Development and Potential (IHDP), Singapore: collaborator

Study Sites (1)

KK Women's and Children's Hospital

Singapore, Singapore

Location

Related Publications (9)

• Loo EXL, Soh SE, Loy SL, Ng S, Tint MT, Chan SY, Huang JY, Yap F, Tan KH, Chern BSM, Tan HH, Meaney MJ, Karnani N, Godfrey KM, Lee YS, Chan JKY, Gluckman PD, Chong YS, Shek LP, Eriksson JG; S-PRESTO Study Group; Chia A, Fogel AM, Goh AEN, Chu AHY, Rifkin-Graboi A, Qiu A, Lee BW, Cheon BK, Vaz C, Henry CJ, Forde CG, Chi C, Koh DXP, Phua DY, Loh DNL, Quah EPL, Tham EH, Law ECN, Magkos F, Mueller-Riemenschneider F, Yeo GSH, Yong HEJ, Chen HY, Tan HH, Pan H, Bever HPSV, Tan HM, Aris IBM, Tay J, Chan JKY, Xu J, Yoong JS, Eriksson JG, Choo JTL, Bernard JY, Huang JY, Lai JS, Tan KML, Godfrey KM, Kwek KYC, McCrickerd K, Narasimhan K, Chong KW, Lee KJ, Chen L, Ling LH, Chen LW, Daniel LM, Shek LP, Fortier MV, Chong MF, Chua MC, Leow MK, Kee MZL, Gong M, Tint MT, Michael N, Lek N, Teoh OH, Mishra P, Li QLJ, Velan SS, Ang SB, Cai S, Goh SH, Lim SB, Tsotsi S, Hsu SC, Toh SES, Sadananthan SA, Tan TH, Yew TW, Gupta V, Rajadurai VS, Han WM, Pang WW, Yuan WL, Zhu Y, Cheung YB, Chan YH, Cheng ZR. Cohort profile: Singapore Preconception Study of Long-Term Maternal and Child Outcomes (S-PRESTO). Eur J Epidemiol. 2021 Jan;36(1):129-142. doi: 10.1007/s10654-020-00697-2. Epub 2020 Nov 21.

  PMID: 33222050 BACKGROUND

• Chu AHY, Padmapriya N, Tan SL, Goh CMJL, Chong YS, Shek LP, Tan KH, Gluckman PD, Yap FKP, Lee YS, Loy SL, Chan JKY, Godfrey KM, Eriksson JG, Chan SY, Bernard JY, Muller-Riemenschneider F. Preconception to postpartum accelerometry-based 24-hour movement behaviors: a prospective cohort study. BMC Public Health. 2026 Jan 7. doi: 10.1186/s12889-025-26034-4. Online ahead of print.

  PMID: 41501702 DERIVED

• Ku CW, Chan HG, Sia AL, Huang C, Quek J, Cheung YB, Tan KML, Lai JS, Godfrey KM, Chan JKY, Yap F, Loy SL. One-carbon metabolism, insulin resistance, and fecundability in a Singapore prospective preconception cohort study. Am J Clin Nutr. 2025 Jul;122(1):335-343. doi: 10.1016/j.ajcnut.2025.04.035. Epub 2025 May 5.

  PMID: 40334751 DERIVED

• Chen L, Mir SA, Bendt AK, Chua EWL, Narasimhan K, Tan KM, Loy SL, Tan KH, Shek LP, Chan J, Yap F, Meaney MJ, Chan SY, Chong YS, Gluckman PD, Eriksson JG, Karnani N, Wenk MR. Plasma lipidomic profiling reveals metabolic adaptations to pregnancy and signatures of cardiometabolic risk: a preconception and longitudinal cohort study. BMC Med. 2023 Feb 13;21(1):53. doi: 10.1186/s12916-023-02740-x.

  PMID: 36782297 DERIVED

• Li LJ, Du R, Loy SL, Chong YS, Chan JKY, Wong TY, Eriksson JG, Huang Z, Zhang C. Retinal microvasculature and risk of spontaneous abortion in multiethnic Southeast Asian women. Fertil Steril. 2022 Oct;118(4):748-757. doi: 10.1016/j.fertnstert.2022.06.033. Epub 2022 Aug 16.

  PMID: 35981917 DERIVED

• Lim SX, Loy SL, Colega MT, Lai JS, Godfrey KM, Lee YS, Tan KH, Yap F, Shek LP, Chong YS, Eriksson JG, Chan JKY, Chan SY, Chong MF. Prepregnancy adherence to plant-based diet indices and exploratory dietary patterns in relation to fecundability. Am J Clin Nutr. 2022 Feb 9;115(2):559-569. doi: 10.1093/ajcn/nqab344.

  PMID: 34626169 DERIVED

• Huang L, Loy SL, Chen WQ, Eriksson JG, Chong YS, Huang Z, Chan JKY, Wong TY, Kramer M, Zhang C, Li LJ. Retinal microvasculature and time to pregnancy in a multi-ethnic pre-conception cohort in Singapore. Hum Reprod. 2021 Oct 18;36(11):2935-2947. doi: 10.1093/humrep/deab197.

  PMID: 34492112 DERIVED

• Lim SX, Colega MT, M Ayob MN, Robinson SM, Godfrey KM, Bernard JY, Lee YS, Tan KH, Yap F, Shek LP, Chong YS, Eriksson JG, Chan JK, Chan SY, Chong MF. Identification and reproducibility of dietary patterns assessed with a FFQ among women planning pregnancy. Public Health Nutr. 2021 Jun;24(9):2437-2446. doi: 10.1017/S1368980021001178. Epub 2021 Mar 22.

  PMID: 33745499 DERIVED

• Loy SL, Ku CW, Lai AEQ, Choo XH, Ho AHM, Cheung YB, Godfrey KM, Chong YS, Gluckman PD, Shek LP, Tan KH, Yap FKP, Chan SY, Chan JKY. Plasma glycemic measures and fecundability in a Singapore preconception cohort study. Fertil Steril. 2021 Jan;115(1):138-147. doi: 10.1016/j.fertnstert.2020.07.014. Epub 2020 Oct 15.

  PMID: 33070964 DERIVED

Related Links

• Study website

Biospecimen

Retention: SAMPLES WITH DNA

Human biological materials such as blood, urine, buccal swabs, saliva, hair, stool, skin microbiome, epithelial swabs (including vaginal, vulval, rectal and breast skin swabs), nasal swabs, breast milk, cord and cord blood, placental samples, subcutaneous fat samples, heel prick blood are collected prospectively from the participants (mother or child) at respective time points, upon their consent.

MeSH Terms

Conditions

Metabolic Diseases; Neurologic Manifestations; Genetic Predisposition to Disease; Skin Diseases; Developmental Origins of Health and Disease

Condition Hierarchy (Ancestors)

Nutritional and Metabolic Diseases; Nervous System Diseases; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Disease Susceptibility; Disease Attributes; Pathologic Processes; Skin and Connective Tissue Diseases; Prenatal Exposure Delayed Effects; Prenatal Injuries; Pregnancy Complications; Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases

Study Officials

• Jerry Kok Yen Chan

  KK Women's and Children's Hospital

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER GOV
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Prof

Study Record Dates

• First Submitted: August 27, 2017
• First Posted: May 22, 2018
• Study Start: February 27, 2015
• Primary Completion (Estimated): December 1, 2029
• Study Completion (Estimated): December 1, 2029
• Last Updated: June 18, 2025

Record last verified: 2025-03

Data Sharing

• IPD Sharing: Will not share

Locations

SG (1)