Interaction of Salivary Proteins and Polyphenols

NCT03501238 | Completed

• 1 other identifier: 082-002
• Study Type: interventional
• Target: 63
• Locations: 1 country
• Sites: 1

Brief Summary

Participants will be chosen through an initial sensory test to find high and low-perceivers of astringency. Selected participants will drink a chocolate milk or milk substitute beverage for one week each with washout weeks in between. At the end of each week participants will taste and rate multiple beverages for their astringency and other sensory properties. Participants will provide saliva samples weekly for proteomic analysis and (potentially) analysis of polyphenols. Participants will also be asked to provide a 24 hour dietary recall once per week. Data will be analyzed to determine if there is a relationship between polyphenol exposure, astringency ratings, and salivary protein composition.

Conditions

Diet Habit; Diet Modification; Taste, Altered; Saliva Altered

Outcome Measures

Primary Outcomes (1)

• Relative salivary proteins in saliva

  Measure relative amounts of salivary proteins in participant saliva.

  1 year

Secondary Outcomes (5)

• Subject Diets

  8 months

• Chocolate milk flavor ratings

  8 months

• Chocolate milk substitute flavor ratings

  8 months

• Chocolate milk substitute with gelatin flavor ratings

  8 months

• Polyphenol content in saliva samples

  8 months

Study Arms (6)

BSG

EXPERIMENTAL

Participant receives bovine milk, then milk substitute, then milk substitute treated with gelatin

Other: Chocolate Milk; Other: Chocolate milk substitute; Other: Chocolate milk substitute with gelatin

BGS

EXPERIMENTAL

Participant receives bovine milk, then milk substitute with gelatin, then milk substitute

Other: Chocolate Milk; Other: Chocolate milk substitute; Other: Chocolate milk substitute with gelatin

SBG

EXPERIMENTAL

Participant receives milk substitute, then bovine milk, then milk substitute with gelatin.

Other: Chocolate Milk; Other: Chocolate milk substitute; Other: Chocolate milk substitute with gelatin

SGB

EXPERIMENTAL

Participant receives milk substitute, then milk substitute with gelatin, then bovine milk

Other: Chocolate Milk; Other: Chocolate milk substitute; Other: Chocolate milk substitute with gelatin

GSB

EXPERIMENTAL

Participant receives milk substitute with gelatin, then milk substitute, then bovine milk.

Other: Chocolate Milk; Other: Chocolate milk substitute; Other: Chocolate milk substitute with gelatin

GBS

EXPERIMENTAL

Participant receives milk substitute with gelatin, then bovine milk, then milk substitute.

Other: Chocolate Milk; Other: Chocolate milk substitute; Other: Chocolate milk substitute with gelatin

Interventions

Chocolate Milk OTHER

Chocolate bovine milk will be consumed everyday for 1 week. Milks will be store-bought, with added sucrose and added cocoa powder.

BGS; BSG; GBS; GSB; SBG; SGB

Chocolate milk substitute OTHER

Chocolate milk substitute will be consumed everyday for 1 week. Milks will be store-bought, with added sucrose and added cocoa powder.

BGS; BSG; GBS; GSB; SBG; SGB

Chocolate milk substitute with gelatin OTHER

Chocolate milk substitute with gelatin added will be consumed everyday for 1 week. Milks will be store-bought, with added sucrose and added cocoa powder.

BGS; BSG; GBS; GSB; SBG; SGB

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• High or low perceivers of astringency (from initial screening)

You may not qualify if:

• Current smokers (within 30 days), dairy/nut/cocoa allergies, piercings in mouth, mid-range perceiver of astringency, issues with taste, smell, swallowing or choking.

Sponsors & Collaborators

• Purdue University: lead

Study Sites (1)

Purdue University

West Lafayette, Indiana, 47907, United States

Location

MeSH Terms

Conditions

Feeding Behavior; Dysgeusia

Interventions

Gelatin

Condition Hierarchy (Ancestors)

Behavior, Animal; Behavior; Taste Disorders; Sensation Disorders; Neurologic Manifestations; Nervous System Diseases; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Scleroproteins; Proteins; Amino Acids, Peptides, and Proteins

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Masking Details: Participants will not know the specifics of each beverage they are taking home.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: May 24, 2017
• First Posted: April 18, 2018
• Study Start: June 8, 2017
• Primary Completion: January 1, 2018
• Study Completion: January 1, 2018
• Last Updated: May 25, 2023

Record last verified: 2023-05

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)