Adipose Tissue Cellular Response to Exercise in Healthy Humans

NCT03314506 | Completed

• 1 other identifier: HUM00131070
• Study Type: interventional
• Target: 40
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to measure the effects of a single session of exercise on changes in the cellular composition of the body fat of humans.

Conditions

Metabolic Disease; Adipose Tissue

Keywords

exercise

Outcome Measures

Primary Outcomes (1)

• Quantifying the change in markers of macrophage abundance in adipose tissue

  The stromovascular fraction will be isolated from subcutaneous lipo-aspirate samples by collagenase digestion and a series of filtration/wash steps (routinely performed by our lab). This isolation protocol has been optimized to yield the minimum number of stromal cells required to identify and recover the following cell populations by fluorescence-activated cell sorting (FACS), listed as cell surface stain = cell type: CD64+/CD11c+ = macrophages FACS will be performed at the University of Michigan Flow Cytometry Core, and sorted cells will be recovered for subsequent gene expression analysis using the StepOnePlus real time PCR system (Life Technologies) with fluorescent probe-based assays.

  1 day

Secondary Outcomes (1)

• Quantifying the change in markers of endothelial cell and pre-adipocytes in adipose tissue

  1 day

Study Arms (1)

Trial

EXPERIMENTAL

Subjects will need to come to the Substrate Metabolism Laboratory in the morning around 7:00 AM. After about a 15-30 minute rest, the study team will collect a blood sample from the subject's hand or forearm. The study team will also obtain a small sample of fat tissue from the area just underneath the skin near the belly button. Next, subjects will exercise on a treadmill at a moderate intensity for about 1 hour. Immediately after exercising the study team will collect a blood sample. The subject will remain resting in the laboratory for 3 hours after exercise, and then the study team will collect another blood and fat tissue sample. Upon completion, the subject will be provided a snack before leaving the laboratory.

Other: Exercise

Interventions

Exercise OTHER

Subjects will exercise on a treadmill for 1 hour at a moderate intensity (approximately 70% of their predicted VO2peak).

Trial

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age: 18-40
• Body Mass Index: 20-27 kg/m2
• Regular exercise (less than or equal to 3 days/week of aerobic exercise; 30-60 minutes/session at moderate and vigorous intensities)
• Women must have regularly occurring menses and must be premenopausal

You may not qualify if:

• Blood pressure greater than 140/90 mm Hg
• Pregnant or lactating
• Evidence/history of cardiovascular or metabolic disease
• Medications known to affect lipid or glucose metabolism, or inflammation

Sponsors & Collaborators

• University of Michigan: lead

Study Sites (1)

University of Michigan

Ann Arbor, Michigan, 48109, United States

Location

MeSH Terms

Conditions

Metabolic Diseases; Motor Activity

Interventions

Exercise

Condition Hierarchy (Ancestors)

Nutritional and Metabolic Diseases; Behavior

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Jeffrey F Horowitz

  University of Michigan

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor, Movement Science, School of Kinesiology

Study Record Dates

• First Submitted: September 27, 2017
• First Posted: October 19, 2017
• Study Start: November 1, 2017
• Primary Completion: August 8, 2019
• Study Completion: August 8, 2019
• Last Updated: March 21, 2022

Record last verified: 2022-03

Locations

US (1)