Effect of Whey Protein Hydrolysate on Human Muscle Protein Synthesis

NCT03313830 | Completed

• 1 other identifier: 17-0086
• Study Type: interventional
• Target: 11
• Locations: 1 country
• Sites: 1

Brief Summary

Muscle protein synthesis can be stimulated by ingestion of protein sources, such as whey, casein or soy. Protein supplementation can be useful to restore protein turnover after exercise but also to preserve skeletal muscle mass and function in aging adults. Ingestion of large doses of essential amino acids (EAA) or certain protein supplements may be an effective strategy to induce muscle protein synthesis. However, in many cases, it may not be practical or feasible to consume a large volume of amino acids or protein required for an effective response by muscle. Several evidences show how reduced strength and muscle mass, even in early life, are predictors of early mortality which explicit the importance of developing more effective methods to improve muscle quality. Therefore, identifying the better sources of protein that have higher anabolic potency is of high significance. The goal of this study is to determine the anabolic potency and efficacy of a new and novel Whey Protein Hydrolysate mixture (WPH) on skeletal muscle protein synthesis in healthy young subjects (age 20-35 yr). Previous studies on rats indicate WPH induces significant increases in muscle protein synthesis compared with carbohydrates or whey-amino acid mixture. WPH contains mostly peptides, which have physiological effects and could be absorbed more rapidly. Preliminary data from preclinical study has also demonstrated that WPH can stimulate muscle protein synthesis at lower doses compared with intact whey proteins. Thus, WPH could be absorbed more rapidly and may maximally stimulate muscle protein synthesis. Although there is substantial data on the individual effects of BCCA and intact protein such as whey, there have been no clinical investigations that have explored the efficacy of WPH for stimulating muscle protein synthesis in humans. Therefore, the investigators propose that WPH will increase muscle protein synthesis. They will compare the response of WHP to the response of WHEY when equal protein is provided in both treatments. 10 healthy subjects will be recruited and will receive both WPH and WHEY supplementation in a single blind crossover design. Muscle protein synthesis will be measured on both occasions. This acute study will allow to determine whether low dose WPH supplementation will be an effective nutritional treatment to stimulate muscle protein synthesis in young adults.

Conditions

Healthy

Keywords

Whey; Muscle Protein Synthesis

Outcome Measures

Primary Outcomes (1)

• muscle protein synthesis

  measure muscle protein turnover by stable isotope infusion trial

  change from Baseline muscle protein synthesis at 3 hours after mixture ingestion

Study Arms (2)

Whey Protein Hydrolysate (WPH)

EXPERIMENTAL

The intervention consists of Whey Protein Hydrolysate (WPH) ingestion by young healthy subjects to determine rates of muscle protein synthesis.

Dietary Supplement: Whey Protein Hydrolysate (WPH)

Intact Whey Protein (WHEY)

EXPERIMENTAL

The intervention consists of Intact Whey Protein (WHEY) ingestion by young healthy subjects to determine rates of muscle protein synthesis.

Dietary Supplement: Intact Whey Protein (WHEY)

Interventions

Whey Protein Hydrolysate (WPH) DIETARY_SUPPLEMENT

Subjects will ingest WPH on one occasion.

Whey Protein Hydrolysate (WPH)

Intact Whey Protein (WHEY) DIETARY_SUPPLEMENT

Subject will ingest WHEY on another occasion.

Intact Whey Protein (WHEY)

Eligibility Criteria

• Age: 20 Years - 35 Years
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Men age 20-35 yrs
• Stable body weight for at least 1 year
• Able to provide written consent and understand the study requirements and procedures

You may not qualify if:

• Exercise training
• Significant heart, liver, kidney, blood, respiratory disease or thyroid issues
• Peripheral vascular disease
• Orthopedic injury
• Diabetes mellitus or other untreated endocrine disease
• Active cancer (all groups) and history of cancer
• Acute infectious disease or history of chronic infections
• Neurologic Injury or disease
• Recent systemic treatment with anabolic steroids, or corticosteroids.
• Alcohol or drug abuse
• Tobacco use
• Malnutrition
• Obesity
• Low hemoglobin levels
• Food allergies

Sponsors & Collaborators

• The University of Texas Medical Branch, Galveston: lead
• Meiji Co., Ltd.: collaborator

Study Sites (1)

UTMB

Galveston, Texas, 77555, United States

Location

Related Publications (1)

• Moro T, Brightwell CR, Velarde B, Fry CS, Nakayama K, Sanbongi C, Volpi E, Rasmussen BB. Whey Protein Hydrolysate Increases Amino Acid Uptake, mTORC1 Signaling, and Protein Synthesis in Skeletal Muscle of Healthy Young Men in a Randomized Crossover Trial. J Nutr. 2019 Jul 1;149(7):1149-1158. doi: 10.1093/jn/nxz053.

  PMID: 31095313 DERIVED

Study Officials

• Blake Rasmussen, PhD

  UTMB

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: October 6, 2017
• First Posted: October 18, 2017
• Study Start: October 7, 2017
• Primary Completion: November 6, 2020
• Study Completion: November 6, 2020
• Last Updated: November 10, 2020

Record last verified: 2020-11

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)