Effects of Protein Timing and Resistance Exercise on Fat Metabolism
The Effect of Protein Timing and Resistance Exercise on Lipolysis and Fat Oxidation in Resistance-Trained Women
1 other identifier
interventional
13
1 country
1
Brief Summary
The extent to which resistance exercise training and protein timing effects localized (belly fat) and systemic (whole body) fat metabolism in resistance-trained women is unknown. Therefore, this study will assess subcutaneous abdominal adipose tissue lipolytic rate and whole body substrate utilization over the course of two trials: 1) consuming casein protein during the day, and 2) consuming casein protein at night, before bed.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for not_applicable healthy
Started Mar 2017
Typical duration for not_applicable healthy
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
Click on a node to explore related trials.
Study Timeline
Key milestones and dates
First Submitted
Initial submission to the registry
January 23, 2017
CompletedStudy Start
First participant enrolled
March 20, 2017
CompletedPrimary Completion
Last participant's last visit for primary outcome
February 28, 2018
CompletedStudy Completion
Last participant's last visit for all outcomes
February 28, 2018
CompletedFirst Posted
Study publicly available on registry
June 29, 2018
CompletedJuly 6, 2018
July 1, 2018
12 months
January 23, 2017
July 5, 2018
Conditions
Keywords
Outcome Measures
Primary Outcomes (1)
Lipolysis
Lipolysis is the way that fat cells mobilize the stored fat to enter into blood circulation. Lipolysis can be measured using a technique called microdialysis. In short, a semi-permeable probe is inserted into the subcutaneous abdominal adipose tissue (SCAAT, or belly fat). Saline combined with a minute amount of ethanol is perfused into the SCAAT, and interstitial fluid is collected out. The concentration of ethanol in the interstitial fluid sample is an indicator of blood flow within the tissue. Glycerol and free fatty acid concentration is measured to indicated rate of fat metabolism (specifically glycerol). The area under the curve for glycerol concentration over time (AUCgly) will be calculated for each of time point. Microdialysis dialysate samples will be measured during the NP and DP trials at fifteen separate time points throughout each visit.
18 hours
Secondary Outcomes (2)
Hormone Concentrations
18 hours
Fat oxidation
18 hours
Study Arms (3)
RT on Fat Metabolism
EXPERIMENTALDetermine the extent to which a full-body acute RT protocol will affect intra-RT and post-RT SCAAT lipolytic rate, and post-RT whole-body substrate utilization in RT women compared to baseline measures (independent of Aims 2 and 3).
PRO Timing on Fat Metabolism
EXPERIMENTALAssess the differences in overnight and next morning SCAAT lipolytic rate and next-morning whole-body substrate utilization compared to baseline between acute NP and DP consumption trials after a RT bout in RT women.
PRO Timing on Markers of Fat Metabolism
EXPERIMENTALAssess the differences in overnight and next morning metabolic biomarkers of fat metabolism compared to baseline between acute nighttime PRO (NP) consumption versus daytime PRO (DP) consumption trials after a RT bout in RT women.
Interventions
The micellar casein protein supplement (PRO, total protein content: 30 g, 120 kcals; 30 g micellar protein) and non-caloric sensory-matched placebo (PLA, 0kcals) will be specifically formulated by Dymatize® Nutrition FrieslandCampina® and consumed in a randomized order. Participants will consume the daytime supplement 30 minutes after RT and the nighttime supplement about 30 minutes before bedtime. The supplements will be premixed, taste-matched (chocolate), and provided in opaque shaker bottles.
Resistance exercise training (RT) will target the full body and exercises will be performed using a barbell. Exercises will be performed in the following order: back squat, bench press, Romanian deadlift, bent-over row, shoulder press, and reverse lunges)
Eligibility Criteria
You may qualify if:
- Healthy
- Resistance-trained (able to squat 1.0 times their body weight, and bench press 0.75 times body weight, adapted from ACSM Guidelines and piloted in our laboratory)
- Normal weight (BMI = 18.5-25 kg/m2; body fat \<33%)
- Normally menstruating (on or off oral contraceptives)
- Females.
You may not qualify if:
- Uncontrolled thyroid dysfunction (not on medication)
- Musculoskeletal disorders
- Currently smoke
- Have milk allergies
- Currently take medications or supplements known to affect substrate metabolism.
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
Institute for Sports Sciences and Medicine, Florida State University
Tallahassee, Florida, 32306, United States
Related Publications (1)
Allman BR, Morrissey MC, Kim JS, Panton LB, Contreras RJ, Hickner RC, Ormsbee MJ. Lipolysis and Fat Oxidation Are Not Altered with Presleep Compared with Daytime Casein Protein Intake in Resistance-Trained Women. J Nutr. 2020 Jan 1;150(1):47-54. doi: 10.1093/jn/nxz186.
PMID: 31504693DERIVED
MeSH Terms
Interventions
Intervention Hierarchy (Ancestors)
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- RANDOMIZED
- Masking
- DOUBLE
- Who Masked
- PARTICIPANT, INVESTIGATOR
- Masking Details
- The supplements will be made by a third party person and premixed, taste-matched, and provided in opaque shaker bottles.
- Purpose
- BASIC SCIENCE
- Intervention Model
- CROSSOVER
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Assistant Professor
Study Record Dates
First Submitted
January 23, 2017
First Posted
June 29, 2018
Study Start
March 20, 2017
Primary Completion
February 28, 2018
Study Completion
February 28, 2018
Last Updated
July 6, 2018
Record last verified: 2018-07
Data Sharing
- IPD Sharing
- Will not share