NCT02918292

Brief Summary

This study is a prospective, multicenter phase II study with patients receiving haploidentical transplantation for Severe Aplastic Anemia (SAA). The primary objective is to assess overall survival (OS) at 1 year post-hematopoietic stem cell transplantation (HSCT).

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
32

participants targeted

Target at P25-P50 for phase_2

Timeline
Completed

Started Jul 2017

Typical duration for phase_2

Geographic Reach
1 country

28 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

September 27, 2016

Completed
1 day until next milestone

First Posted

Study publicly available on registry

September 28, 2016

Completed
9 months until next milestone

Study Start

First participant enrolled

July 3, 2017

Completed
4.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

August 17, 2021

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

August 17, 2021

Completed
11 months until next milestone

Results Posted

Study results publicly available

July 12, 2022

Completed
Last Updated

March 31, 2026

Status Verified

March 1, 2026

Enrollment Period

4.1 years

First QC Date

September 27, 2016

Results QC Date

April 29, 2022

Last Update Submit

March 18, 2026

Conditions

Keywords

Haploidentical Bone MarrowHematopoietic Stem Cell Transplant (HSCT)Severe Aplastic Anemia (SAA)Antithymocyte Globulin (ATG)

Outcome Measures

Primary Outcomes (1)

  • Percentage of Participants With Overall Survival (OS)

    Overall survival (OS) is the primary endpoint of this study. The time to this event is the time from transplant to death from any cause or last follow-up or 1 year from transplant, whichever occurs first. The one-year OS probability and its 95% confidence interval were estimated using the Kaplan-Meier estimator and Greenwood's formula.

    1 year

Secondary Outcomes (19)

  • Percentage of Participants With Neutrophil Recovery

    Day 28 and 56

  • Percentage of Participants With Platelet Recovery

    Day 100

  • Participants Alive With Sustained Engraftment

    1 year

  • Percentage of Participants With Graft-Failure-Free Survival

    1 year

  • Percentage of Participants With Primary Graft Failure

    Day 56

  • +14 more secondary outcomes

Study Arms (1)

Haplo Bone Marrow HSCT

EXPERIMENTAL

Patients will be treated with a preparative regimen of Antithymocyte Globulin (ATG) (4.5 mg/kg), fludarabine (150 mg/m\^2), cyclophosphamide (29 mg/kg), and low dose total body irradiation (TBI) (200 cGy) before undergoing the haplo HSCT. GVHD prophylaxis will be with post-HSCT cyclophosphamide (100 mg/kg), tacrolimus, and mycophenolate mofetil (MMF). G-CSF will be administered post-transplant.

Drug: Antithymocyte Globulin (ATG)Drug: FludarabineDrug: CyclophosphamideRadiation: Total Body Irradiation (TBI)Procedure: Haplo HSCTDrug: TacrolimusDrug: Mycophenolate mofetil (MMF)Drug: G-CSF

Interventions

Administration of ATG will be 0.5 mg/kg IV on Day -9 over 6 hours and 2 mg/kg IV on Days -8 and -7 over 4 hours.

Also known as: Thymoglobulin®, rATG
Haplo Bone Marrow HSCT

Cyclophosphamide dose will be 14.5 mg/kg IV daily for 2 days (Day -6 to Day -5) prior to transplantation and 50 mg/kg IV daily for 2 days (Day +3 to Day +4) after transplantation.

Also known as: Cytoxan®
Haplo Bone Marrow HSCT

TBI is to be delivered in a single dose of 200 cGy on Day -1.

Haplo Bone Marrow HSCT
Haplo HSCTPROCEDURE

Eligible patients without a fully matched related or unrelated donor available will undergo haploidentical bone marrow transplant.

Haplo Bone Marrow HSCT

Tacrolimus should be started on Day +5 and administered to maintain a level of 10-15 ng/mL.

Also known as: Prograf®
Haplo Bone Marrow HSCT

MMF dose will be 15 mg/kg PO three times a day (TID) up to 1 gm TID (or IV equivalent) starting on Day +5.

Also known as: Cellcept®
Haplo Bone Marrow HSCT
G-CSFDRUG

G-CSF will be given IV or SQ starting on Day +5 at 5 mcg/kg/day until ANC is \> 1500 for 3 days.

Also known as: Filgrastim, Neupogen®
Haplo Bone Marrow HSCT

Fludarabine dose will be 30 mg/m\^2 IV daily for 5 days from Day -6 to Day -2.

Also known as: Fludara®
Haplo Bone Marrow HSCT

Eligibility Criteria

AgeUp to 75 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Patient is \< 75 years of age at time of enrollment.
  • Confirmed diagnosis of SAA, either from initial diagnosis or follow-up assessments, defined as:
  • Bone marrow cellularity \< 25% or marrow cellularity \< 50% but with \< 30% residual hematopoietic cells.
  • Two out of three of the following (in peripheral blood): Neutrophils \< 0.5 x10\^9/L, Platelets \< 20 x10\^9/L, or Reticulocyte count \< 20 x10\^9/L (\<60 x 10\^9/L using an automated analysis)
  • No suitable fully matched related sibling donor (6/6 match for human leukocyte antigen (HLA)-A and B at intermediate or high resolution and DRB1 at high resolution using DNA-based typing) available.
  • Failed at least one trial of immunosuppressive therapy (IST) by being refractory or having relapsed. IST could have included ATG based regimens, calcineurin inhibitors and/or other higher dose therapy directed at the treatment of primary SAA.
  • Available relative of the patient who is a haploidentical match, including biological parents, siblings or half siblings, children, uncles/aunts, first cousins, etc. Eligible haploidentical donors will have 2-4 mismatches if HLA-A, -B, -C, and -DRB1 typing is used; 2-5 mismatches if HLA-A, -B, -C, -DRB1, and -DQB1 typing is used; and 2-6 mismatches if HLA-A, -B, -C, -DRB1, -DQB1, and -DPB1 typing is used. A unidirectional mismatch in either the graft versus host or host versus graft direction is considered a mismatch. The donor and recipient must demonstrate that they are a full haplotype match by being identical at a minimum of one allele (at high resolution DNA-based typing) at the following genetic loci: HLA-A, -B, -C, and DRB1 if 8 allele typing is used; HLA-A, -B, -C, -DRB1, and -DQB1 if 10 allele typing is used; and HLA-A, -B, -C, -DRB1-, DQB1, and -DPB1 is 12 allele typing is used by the local center. See Section 2.4 for additional information.
  • Patient and/or legal guardian must sign informed consent for HSCT.
  • The haplo donor and/or legal guardian must be able to sign informed consent documents.
  • The potential haplo donor must be willing and able to donate bone marrow.
  • The weight of the haplo donor must be ≥ 20 kg.
  • Adequate organ function defined as:
  • Cardiac: Left ventricular ejection fraction (LVEF) at rest ≥ 40%. For patients aged \< 13 years, shortening fraction (SF) ≥ 26% by echocardiogram or Multi Gated Acquisition Scan (MUGA) may be substituted for LVEF.
  • Hepatic: Total bilirubin \< 3.0 x the upper limit of normal (ULN) for age (patients who have been diagnosed with Gilbert's Disease are allowed to exceed this limit) and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) \< 5.0 x ULN for age.
  • Renal: For patients \> 13.0 years of age at the time of enrollment: estimated creatinine clearance \> 50 mL/minute (using the Cockcroft-Gault formula and actual body weight). For patients \< 13.0 years of age at enrollment: Glomerular Filtration Rate (GFR) estimated by the updated Schwartz formula ≥ 90 mL/min/1.73 m2. If the estimated GFR is \< 90 mL/min/1.73 m\^2, then renal function must be measured by 24-hour creatinine clearance or nuclear GFR, and must be \> 50 mL/min/1.73 m\^2.
  • +3 more criteria

You may not qualify if:

  • Inherited bone marrow failure syndromes such as Fanconi anemia must be ruled out according to center standard.
  • Clonal cytogenetic abnormalities consistent with pre-myelodysplastic syndrome (pre-MDS) or MDS on marrow examination (e.g. Monosomy 7).
  • Presence of anti-donor HLA antibodies (positive anti-donor HLA antibody is defined as a positive cross-match test of any titer by complement-dependent cytotoxicity or flow cytometric testing or the presence of anti-donor HLA antibody to the high expression loci HLA-A, B, C, DRB1, or DPB1 with mean fluorescence intensity (MFI) \> 1000 by solid phase immunoassay).
  • Prior allogeneic stem cell transplant.
  • Prior solid organ transplant.
  • Known life-threatening reaction (i.e., anaphylaxis) to Thymoglobulin® that would prohibit use for the patient as this study requires use of the Thymoglobulin® preparation of ATG.
  • Uncontrolled bacterial, viral, or fungal infection at the time of enrollment. Uncontrolled is defined as currently taking medication and with progression or no clinical improvement on adequate medical treatment.
  • Seropositive for the human immunodeficiency virus (HIV).
  • Active Hepatitis B or C determined by a detectable viral load of HBV or HCV.
  • Female patients who are pregnant (per institutional practice) or breast-feeding.
  • Prior malignancies except resected basal cell carcinoma or treated cervical carcinoma in situ. Cancer treated with curative intent \> 5 years previously will be allowed. Cancer treated with curative intent ≤ 5 years previously will not be allowed unless approved by the Protocol Chairs and/or Protocol Officer.
  • Alemtuzumab or ATG within 2 weeks of enrollment.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (28)

City of Hope National Medical Center

Duarte, California, 91010, United States

Location

Children's Hospital of Los Angeles

Los Angeles, California, 90027, United States

Location

Children's Hospital and Research Center Oakland

Oakland, California, 94618, United States

Location

Stanford Hospital and Clinics

Stanford, California, 94305, United States

Location

Children's National Medical Center

Washington D.C., District of Columbia, 20010, United States

Location

University of Florida College of Medicine

Gainesville, Florida, 32610, United States

Location

Miami Children's Hospital

Miami, Florida, 33155, United States

Location

All Children's Hospital

St. Petersburg, Florida, 33701, United States

Location

H. Lee Moffitt Cancer Center

Tampa, Florida, 33624, United States

Location

Northside Hospital

Atlanta, Georgia, 30342, United States

Location

Indiana University Medical Center/ Riley Hospital for Children

Indianapolis, Indiana, 46202, United States

Location

University of Louisville/Kosair Children's Hospital

Louisville, Kentucky, 40202, United States

Location

Johns Hopkins Unversity

Baltimore, Maryland, 21231, United States

Location

University of Michigan Medical Center

Ann Arbor, Michigan, 48105, United States

Location

Karmanos Cancer Institute/Children's Hospital of Michigan

Detroit, Michigan, 48201, United States

Location

Children's Mercy Hospital and Clinics

Kansas City, Missouri, 64108, United States

Location

Washington University/Barnes Jewish Hospital

St Louis, Missouri, 63110, United States

Location

Hackensack University Medical Center

Hackensack, New Jersey, 07601, United States

Location

Roswell Park Cancer Institute

Buffalo, New York, 14263, United States

Location

Duke University Medical Center

Durham, North Carolina, 27705, United States

Location

Oregon Health and Science University

Portland, Oregon, 97239-3098, United States

Location

Penn State College of Medicine/The Milton S. Hershey Medical Center

Hershey, Pennsylvania, 17033, United States

Location

University of Pennsylvania Cancer Center

Philadelphia, Pennsylvania, 19104, United States

Location

Baylor College of Medicine

Houston, Texas, 77030, United States

Location

University of Texas/MD Anderson Cancer Center

Houston, Texas, 77030, United States

Location

Texas Transplant Institute

San Antonio, Texas, 78229, United States

Location

Fred Hutchinson Cancer Research Center

Seattle, Washington, 98109, United States

Location

Children's Hospital of Wisconsin/Midwest Children's Cancer

Milwaukee, Wisconsin, 53211, United States

Location

Related Publications (1)

  • DeZern AE, Eapen M, Wu J, Talano JA, Solh M, Davila Saldana BJ, Karanes C, Horwitz ME, Mallhi K, Arai S, Farhadfar N, Hexner E, Westervelt P, Antin JH, Deeg HJ, Leifer E, Brodsky RA, Logan BR, Horowitz MM, Jones RJ, Pulsipher MA. Haploidentical bone marrow transplantation in patients with relapsed or refractory severe aplastic anaemia in the USA (BMT CTN 1502): a multicentre, single-arm, phase 2 trial. Lancet Haematol. 2022 Sep;9(9):e660-e669. doi: 10.1016/S2352-3026(22)00206-X. Epub 2022 Jul 27.

MeSH Terms

Conditions

Anemia, Aplastic

Interventions

Antilymphocyte Serumthymoglobulinfludarabinefludarabine phosphateCyclophosphamideWhole-Body IrradiationTacrolimusMycophenolic AcidGranulocyte Colony-Stimulating FactorFilgrastim

Condition Hierarchy (Ancestors)

AnemiaHematologic DiseasesHemic and Lymphatic DiseasesBone Marrow Failure DisordersBone Marrow Diseases

Intervention Hierarchy (Ancestors)

Immune SeraAntibodiesImmunoglobulinsImmunoproteinsBlood ProteinsProteinsAmino Acids, Peptides, and ProteinsSerum GlobulinsGlobulinsBiological ProductsComplex MixturesPhosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus CompoundsRadiotherapyTherapeuticsInvestigative TechniquesMacrolidesLactonesCaproatesAcids, AcyclicCarboxylic AcidsFatty AcidsLipidsColony-Stimulating FactorsGlycoproteinsGlycoconjugatesCarbohydratesHematopoietic Cell Growth FactorsCytokinesIntercellular Signaling Peptides and ProteinsPeptidesBiological Factors

Results Point of Contact

Title
Adam Mendizabal, PhD
Organization
The Emmes Company

Study Officials

  • Mary Horowitz, MD, MS

    Center for International Blood and Marrow Transplant Research

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

September 27, 2016

First Posted

September 28, 2016

Study Start

July 3, 2017

Primary Completion

August 17, 2021

Study Completion

August 17, 2021

Last Updated

March 31, 2026

Results First Posted

July 12, 2022

Record last verified: 2026-03

Data Sharing

IPD Sharing
Will share

Results will be published in a manuscript and supporting information submitted to NIH BioLINCC (including data dictionaries, case report forms, data submission documentation, documentation for outcomes dataset, etc where indicated).

Shared Documents
STUDY PROTOCOL, ICF
Time Frame
Within 6 months of official study closure at participating sites.
Access Criteria
Available to the public
More information

Locations