NCT02891603

Brief Summary

The purpose of this study is to examine a new approach to preventing a serious problem after transplant called graft vs. host disease (abbreviated as GVHD). This is a 3 arm sequential phase I/II, study of Pacritinib with Sirolimus and Tacrolimus (PAC/SIR/TAC) for the prevention of acute GVHD after matched related and unrelated allogeneic hematopoietic cell transplantation (alloHCT).

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
40

participants targeted

Target at P50-P75 for phase_1

Timeline
Completed

Started Jun 2017

Longer than P75 for phase_1

Geographic Reach
1 country

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

September 1, 2016

Completed
6 days until next milestone

First Posted

Study publicly available on registry

September 7, 2016

Completed
9 months until next milestone

Study Start

First participant enrolled

June 8, 2017

Completed
4.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 28, 2022

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

April 18, 2022

Completed
1.5 years until next milestone

Results Posted

Study results publicly available

October 25, 2023

Completed
Last Updated

April 2, 2026

Status Verified

March 1, 2026

Enrollment Period

4.6 years

First QC Date

September 1, 2016

Results QC Date

July 3, 2023

Last Update Submit

March 31, 2026

Conditions

Outcome Measures

Primary Outcomes (1)

  • STAT Activity

    STAT3 activity in circulating CD4+ T-cells. This is equivalent to 5.5 tablespoons of blood for each assessment. Peripheral blood mononuclear cells (PBMC) will be isolated by Ficoll density gradient. PBMCs will be stimulated with IL-6 for 20 minutes to activate STAT3. Phosphoproteins will be analyzed within T-cells by flow cytometry. Result reported is %pSTAT3+CD4+T cells at day +21.

    up to 21 days

Secondary Outcomes (1)

  • Incidence of Acute GVHD

    up to 100 days

Study Arms (3)

Phase 1, Level 1: Pacritinib with Sirolimus and Tacrolimus

EXPERIMENTAL

After standard of care allogenic hematopoietic cell transplantation, Pacritinib will be added to standard treatment with Sirolimus and Tacrolimus (PAC/SIR/TAC). 100 mg Pacritinib will begin taken by mouth the day of the participant's transplant (Day 0) and will continue until 70 days after the transplant.. Sirolimus will be given the day before transplant and continued daily for at least one year. Tacrolimus will begin 3 days before transplant and will be given for at least 50 days.

Drug: PacritinibDrug: SirolimusDrug: TacrolimusProcedure: Allogenic hematopoietic cell transplant (alloHCT)

Phase 1, Level 2: Pacritinib with Sirolimus and Tacrolimus

EXPERIMENTAL

After standard of care allogenic hematopoietic cell transplantation, Pacritinib will be added to standard treatment with Sirolimus and Tacrolimus (PAC/SIR/TAC). 100 mg Pacritinib will begin taken by mouth twice daily starting the day of the participant's transplant (Day 0) and continuing until 70 days after the transplant.. Sirolimus will be given the day before transplant and continued daily for at least one year. Tacrolimus will begin 3 days before transplant and will be given for at least 50 days.

Drug: PacritinibDrug: SirolimusDrug: TacrolimusProcedure: Allogenic hematopoietic cell transplant (alloHCT)

Phase 2: Pacritinib with Sirolimus and Tacrolimus

EXPERIMENTAL

After standard of care allogenic hematopoietic cell transplantation, Pacritinib will be added to standard treatment with Sirolimus and Tacrolimus (PAC/SIR/TAC). Patients will take Pacritinib at the MTD: 100 mg Pacritinib will begin taken by mouth twice daily starting the day of the participant's transplant (Day 0) and continuing until 70 days after the transplant.. Sirolimus will be given the day before transplant and continued daily for at least one year. Tacrolimus will begin 3 days before transplant and will be given for at least 50 days.

Drug: PacritinibDrug: SirolimusDrug: TacrolimusProcedure: Allogenic hematopoietic cell transplant (alloHCT)

Interventions

Sirolimus (SIR) will be administered and dosed according to Moffitt Cancer Center, Department of Blood and Marrow Transplantation standard practice. Attending physician discretion is permitted with regard to timing, rapidity, and completion of SIR taper. SIR levels will be monitored according to program standard operating procedures. Dose modifications of SIR for concurrent use of CYP3A4 inhibitors or inducers will be based on program standard operating procedures.

Also known as: Rapamune
Phase 1, Level 1: Pacritinib with Sirolimus and TacrolimusPhase 1, Level 2: Pacritinib with Sirolimus and TacrolimusPhase 2: Pacritinib with Sirolimus and Tacrolimus

Tacrolimus (TAC) will be administered and dosed according to Moffitt Cancer Center, Department of Blood and Marrow Transplantation standard practice. Attending physician discretion is permitted with regard to timing, rapidity, and completion of TAC taper. TAC levels will be monitored according to program standard operating procedures. Dose modifications of TAC for concurrent use of CYP3A4 inhibitors or inducers will be based on program standard operating procedures.

Also known as: Prograf
Phase 1, Level 1: Pacritinib with Sirolimus and TacrolimusPhase 1, Level 2: Pacritinib with Sirolimus and TacrolimusPhase 2: Pacritinib with Sirolimus and Tacrolimus

Pacritinib Dose and Schedule: 200 mg twice a day (BID) orally from day 0 until day +100. PAC will be tapered to 50% of the total dose at day +70, then 25% of total dose at day +84, then stop at day +100 (+/- 7 days).

Also known as: PAC, tyrosine kinase inhibitor (TKI)
Phase 1, Level 1: Pacritinib with Sirolimus and TacrolimusPhase 1, Level 2: Pacritinib with Sirolimus and TacrolimusPhase 2: Pacritinib with Sirolimus and Tacrolimus

Patients will undergo allogenic hematopoietic cell transplant (alloHCT) as a part of their standard of care treatment.

Phase 1, Level 1: Pacritinib with Sirolimus and TacrolimusPhase 1, Level 2: Pacritinib with Sirolimus and TacrolimusPhase 2: Pacritinib with Sirolimus and Tacrolimus

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Must have an available 8/8 HLA-A, -B, -C, and -DRB1 matched-related or unrelated donor allogeneic hematopoietic peripheral blood stem cell graft.
  • Signed informed consent.
  • Acute myeloid leukemia, myelodysplasia, acute lymphoblastic leukemia, chronic myeloid leukemia, chronic lymphocytic leukemia, myeloproliferative neoplasms, Hodgkin lymphoma, or non-Hodgkin lymphoma requiring a matched allogeneic hematopoietic stem cell transplantation (HSCT). Acute Leukemia (AML or ALL) must be in complete remission defined as: \<5% marrow blasts with no morphologic evidence of leukemia, no peripheral blasts, marrow \>20% cellular, and peripheral absolute neutrophil count \>1000/uL (platelet recovery is not required). Myelodysplasia (MDS) and chronic myeloid leukemia (CML): Must have \<5% marrow blasts. Myeloproliferative neoplasms (MPN): Must have \<5% peripheral / marrow blasts. Note: Prior use of a JAK2 inhibitor is allowed up to 4 weeks before day 0 of alloHCT. Hodgkin and non-Hodgkin lymphoma: Must have chemosensitive disease.
  • Adequate vital organ function.
  • Performance status: Karnofsky Performance Status Score ≥ 80%.
  • Donor Eligibility:
  • Eligible donors will include healthy sibling, relative or unrelated donors that are matched with the patient at HLA-A, B, C, and DRB1 by high resolution typing as defined by the Collaborative Trials Network.

You may not qualify if:

  • Active infection not controlled with appropriate antimicrobial therapy.
  • History of HIV, hepatitis B, or active hepatitis C infection.
  • Anti-thymocyte globulin, alemtuzumab, bortezomib, or post-transplant cyclophosphamide as part of GVHD prophylaxis.
  • Sorror's co-morbidity factors with total score \>4.
  • Any patient anticipating or scheduled to receive a tyrosine kinase inhibitor, FLT3 inhibitor, or JAK2 inhibitor (outside of this study) post-HCT.
  • QTc\>450ms per Fridericia's correction.
  • Thrombin time (TT), prothrombin time (PT), or partial thromboplastin time (PTT) \>2x upper limit of normal (ULN).
  • Grade 3 or higher recent (within the past 6 months) or ongoing non-QTc cardiac adverse events/comorbidities.
  • Grade 3 or higher recent or ongoing cardiac dysrhythmias, family history of long QT.
  • syndrome, or serum potassium \<3.0 mEq/L that is persistent and refractory to correction.
  • Grade 3 or higher recent or ongoing bleeding events.
  • Symptomatic or uncontrolled cardiovascular disease, myocardial infarction or severe/unstable angina within the past 6 months, or New York Heart Association (NYHA) Class III or IV congestive heart failure.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

H. Lee Moffitt Cancer Center and Research Institute

Tampa, Florida, 33612, United States

Location

University of Minnesota

Minneapolis, Minnesota, 55455, United States

Location

Related Publications (2)

  • Pidala J, Walton K, Elmariah H, Kim J, Mishra A, Bejanyan N, Nishihori T, Khimani F, Perez L, Faramand RG, Davila ML, Nieder ML, Sagatys EM, Holtan SG, Lawrence NJ, Lawrence HR, Blazar BR, Anasetti C, Sebti SM, Betts BC. Pacritinib Combined with Sirolimus and Low-Dose Tacrolimus for GVHD Prevention after Allogeneic Hematopoietic Cell Transplantation: Preclinical and Phase I Trial Results. Clin Cancer Res. 2021 May 15;27(10):2712-2722. doi: 10.1158/1078-0432.CCR-20-4725. Epub 2021 Mar 22.

  • Betts BC, Bastian D, Iamsawat S, Nguyen H, Heinrichs JL, Wu Y, Daenthanasanmak A, Veerapathran A, O'Mahony A, Walton K, Reff J, Horna P, Sagatys EM, Lee MC, Singer J, Chang YJ, Liu C, Pidala J, Anasetti C, Yu XZ. Targeting JAK2 reduces GVHD and xenograft rejection through regulation of T cell differentiation. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1582-1587. doi: 10.1073/pnas.1712452115. Epub 2018 Jan 30.

Related Links

MeSH Terms

Conditions

Graft vs Host Disease

Interventions

11-(2-pyrrolidin-1-ylethoxy)-14,19-dioxa-5,7,26-triazatetracyclo(19.3.1.1(2,6).1(8,12))heptacosa-1(25),2(26),3,5,8,10,12(27),16,21,23-decaeneTyrosine Kinase InhibitorsSirolimusTacrolimus

Condition Hierarchy (Ancestors)

Immune System Diseases

Intervention Hierarchy (Ancestors)

Protein Kinase InhibitorsEnzyme InhibitorsMolecular Mechanisms of Pharmacological ActionPharmacologic ActionsChemical Actions and UsesMacrolidesLactonesOrganic Chemicals

Results Point of Contact

Title
Joseph Pidala, MD, PhD
Organization
Moffitt Cancer Center

Study Officials

  • Joseph Pidala, M.D.

    H. Lee Moffitt Cancer Center and Research Institute

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restriction Type
GT60
Restrictive Agreement
Yes

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
SEQUENTIAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

September 1, 2016

First Posted

September 7, 2016

Study Start

June 8, 2017

Primary Completion

January 28, 2022

Study Completion

April 18, 2022

Last Updated

April 2, 2026

Results First Posted

October 25, 2023

Record last verified: 2026-03

Locations