Changes in Alveolar Macrophage Function During Acute Lung Injury

NCT02693912 | Unknown

• 1 other identifier: wch-150718
• Study Type: observational
• Target: 25
• Locations: 0 countries
• Sites: N/A

Brief Summary

In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Conditions

Acute Lung Injury

Keywords

efferocytosis; macrophage

Outcome Measures

Primary Outcomes (1)

• alveolar macrophages (AMs) phagocytic index

  AMs will be collected and cultured in vitro. Apoptotic neutrophils will then be cocultured with AMs for 1 hour. To measure phagocytosis of apoptotic neutrophils, AMs were further processed and phagocytosed apoptotic neutrophils were detected by confocal fluorescence microscopy as described below under Cell staining and confocal microscopy. The phagocytic index was calculated according to the following formula: phagocytic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.

  7 days after admission

Secondary Outcomes (1)

• 30 days mortality

  30 days after admission

Study Arms (2)

acute lung injury

Patients with a diagnosis of acute lung injury (ALI) under mechanical ventilation.Patients will underwent bronchoscopy for bronchoalveolar lavage fluid.

Procedure: Bronchoscopy

control

Patient under mechanical ventilation without any lung diseases. Patients will underwent bronchoscopy for bronchoalveolar lavage fluid.

Procedure: Bronchoscopy

Interventions

Bronchoscopy PROCEDURE

Bronchoscopy will be implemented under sedation in both groups.

acute lung injury; control

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

ALI and acute respiratory distress syndrome (ARDS) are characterized by an increased permeability of the alveolar-capillary barrier resulting in lung edema with protein-rich fluid, thus resulting in impairment of arterial oxygenation. ALI/ARDS is defined as a lung disease with acute onset, noncardiac, diffuse bilateral pulmonary infiltrates and a paO2/FiO2 ≤ 300 for ALI or a paO2/FiO2 ≤ 200 for ARDS. The ageadjusted incidence of ALI/ARDS is estimated with 86.2 per 100,000 person-years. Despite all innovations in intensive care medicine, the mortality of ARDS remains up to 40%.

You may qualify if:

• Patients in ICU with diagnosed ALI under mechanical ventilation

You may not qualify if:

• Patients with cardiac dysfunction, using steroid drugs or other drugs can that affect efferocytosis

Sponsors & Collaborators

• West China Hospital: lead

Biospecimen

Retention: SAMPLES WITH DNA

bronchoalveolar lavage fluid will be collected which contains immune cells, including macrophages.

MeSH Terms

Conditions

Acute Lung Injury

Interventions

Bronchoscopy

Condition Hierarchy (Ancestors)

Lung Injury; Lung Diseases; Respiratory Tract Diseases

Intervention Hierarchy (Ancestors)

Diagnostic Techniques, Respiratory System; Diagnostic Techniques and Procedures; Diagnosis; Endoscopy; Diagnostic Techniques, Surgical; Minimally Invasive Surgical Procedures; Surgical Procedures, Operative; Pulmonary Surgical Procedures; Thoracic Surgical Procedures

Study Officials

• Jin Liu

  West China Hospital

  STUDY CHAIR

Central Study Contacts

Chunling Jiang

CONTACT

jiang_chunling@yahoo.com

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Dr.

Study Record Dates

• First Submitted: February 11, 2016
• First Posted: February 29, 2016
• Study Start: September 1, 2016
• Primary Completion: December 1, 2017
• Study Completion: August 1, 2018
• Last Updated: February 29, 2016

Record last verified: 2016-02

Data Sharing

• IPD Sharing: Will not share