the Effect of Dopamine on Mechanical Ventilation Induced Lung Injury

NCT03317431 | Completed Results

• 1 other identifier: XH-17-011
• Study Type: observational
• Target: 46
• Locations: 1 country
• Sites: 1

Brief Summary

Dopamine(DA) is a common neurotransmitter that has been known to regulate behavior, movement, cardiovascular，endocrine and gastrointestinal functions, but also functions as an important molecule engaging in the immune systems to possess anti-inflammatory effects. However, its role in ventilator-induced lung injury (VILI) is still unclear. Herein, this study aimed to investigate the therapeutic efficacy of dopamine on ventilation-induced lung endothelial barrier dysfunction and explore the possible underlying molecular mechanisms.

Conditions

Acute Lung Injury

Keywords

dopamine,mechanical ventilation,lung injury

Outcome Measures

Primary Outcomes (1)

• Correlation Coefficient (r) Between Duration of Mechanical Ventilation and DRD1 Expression

  ImageJ software were used to get the Integrated Optical Density(IOD) of the chemiluminescent signal from the membranes of dopamine receptor 1(DRD1). The normalization was carried out by DRD1 IOD/total β-actin IOD for sample loading correction. In determination of the expression level of DRD1 in different time points, the investigators had a bulk preparation of normal placental protein which was set as a control. Analyze the correlation between duration of mechanical ventilation and DRD1 expression. If p value is less than 0.05, then its change is statically significant.

  20min-60min

Secondary Outcomes (4)

• Correlation Coefficient (r) Between Duration of Mechanical Ventilation and DRD2 Expression

  20min-60min

• the Expression of TH in Lung Tissues

  20min-60min

• the Expression of DDC in Lung Tissues

  20min-60min

• the Expression of Ac-a-tubulin in Lung Tissues

  20min-60min

Study Arms (1)

ventilation

patients undergoing selective operation with general anesthesia(GA) and mechanical ventilation(MV)

Other: mechanical ventilation

Interventions

mechanical ventilation OTHER

mechanical ventilation protocol: tidal volume 6-8 ml/kg, positive end-expiratory pressure 5 cmH2O, oxygen concentration 40%; respiratory rate 10-15/min, inspiratory/expiratory ratio 1:1.5.

ventilation

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

patients undergo elective lobectom

You may not qualify if:

• Distant metastases: recent anaesthetics or mechanical ventilation treatment；children；women during pregnancy or lactation; being involved in other clinical subjects.

Sponsors & Collaborators

• Xinhua Hospital, Shanghai Jiao Tong University School of Medicine: lead

Study Sites (1)

Department of Anesthesia, Shanghai Xinhua hospital

Shanghai, Shanghai Municipality, 200082, China

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Gather the lung tissues from patients undergo lobectomy with general anesthesia and mechanical ventilation

MeSH Terms

Conditions

Acute Lung Injury

Interventions

Respiration, Artificial

Condition Hierarchy (Ancestors)

Lung Injury; Lung Diseases; Respiratory Tract Diseases

Intervention Hierarchy (Ancestors)

Airway Management; Therapeutics; Resuscitation; Emergency Treatment; Respiratory Therapy

Results Point of Contact

• Title: Dr.Lai Jiang
• Organization: Department of Anesthesia,Xinhua hospital affiliated to Shanghai Jiaotong University School of Medicine

jianglaimz@sina.com

021-25078999

Study Officials

• Lai Jiang, chief doctor

  Xinhua Hospital affiliated to Medicine school,Shanghai Jiaotong University

  STUDY CHAIR

Publication Agreements

• PI is Sponsor Employee: Yes

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: July 17, 2017
• First Posted: October 23, 2017
• Study Start: March 20, 2017
• Primary Completion: October 22, 2017
• Study Completion: December 1, 2017
• Last Updated: October 22, 2018
• Results First Posted: October 22, 2018

Record last verified: 2017-03

Locations

CN (1)