NCT02007811

Brief Summary

The reconstitution of a functioning immune system after allogeneic stem cell transplantation takes months to years. Particularly memory B-lymphocytes reconstitute poorly with the current conditioning regimes. During the period of intense immune suppression the patients are extremely susceptible to bacterial, fungal and, most importantly, viral infections.The adoptive transfer of B-lymphocytes from the stem-cell donor might significantly enhance humoral immunity for the patient. Aim of the study is to evaluate a new cellular therapy with B-lymphocytes regarding safety. A booster vaccination after B-lymphocyte transfer will evaluate the functionality of the transferred B-lymphocytes in the patient.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
15

participants targeted

Target at below P25 for phase_1

Timeline
Completed

Started Nov 2013

Typical duration for phase_1

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2013

Completed
20 days until next milestone

First Submitted

Initial submission to the registry

November 21, 2013

Completed
20 days until next milestone

First Posted

Study publicly available on registry

December 11, 2013

Completed
2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2015

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2015

Completed
Last Updated

March 26, 2014

Status Verified

March 1, 2014

Enrollment Period

2.1 years

First QC Date

November 21, 2013

Last Update Submit

March 24, 2014

Conditions

Outcome Measures

Primary Outcomes (3)

  • Number of participants with EBV DNA copies/ml plasma higher than 50,000

    for 120 days after administration of study medication

  • Number of participants with signs of a post-transplant lymphoproliferative disorder (PTLD)

    for 120 days after administration of study medication

  • Number of participants with adverse events (AEs), adverse reactions (ARs), serious adverse events (SAEs), serious adverse reactions (SARs) and suspected unexpected serious adverse reaction (SUSARs)

    for 120 days after administration of study medication

Secondary Outcomes (5)

  • Change in the frequency of antibody-producing cells between dose groups

    before and 7 days after preponed single vaccination

  • Change of mean absolute number of B-lymphocytes, naïve B-lymphocytes and memory B-lymphocytes between dose groups.

    1 day before and up to 120 days after administration of study medication

  • Change of antigen-specific antibody concentration in serum/plasma between dose groups

    1 day before and up to 120 days after administration of study medication

  • Change of Cytomegalovirus (CMV) DNA copies/ml plasma between dose groups

    1 day before and up to 120 days after administration of study medication

  • Number of patients with >5,000 CMV DNA copies/ml plasma or with signs of organ infestation by CMV between dose groups.

    up to 120 days after administration of study medication

Study Arms (1)

allogeneic donor derived B-lymphocytes

EXPERIMENTAL
Biological: allogeneic donor derived B-lymphocytes

Interventions

CD3+-depleted, CD19+-enriched, cryopreserved (single administration after day 120 following allogeneic stem cell transplantation, donor-identical) in 4 groups with escalating doses

allogeneic donor derived B-lymphocytes

Eligibility Criteria

Age18 Years - 75 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • patients after allogeneic stem cell transplantation
  • Serostatus for EBV: R-/D- oder R+/D- oder R+/D+

You may not qualify if:

  • Serostatus for EBV: R-/D+
  • Severe acute Graft versus Host Disease (GvHD) (Glucksberg grade III und IV)
  • Chronic GvHD in middle- or high-risk group according to NIH staging
  • Rituximab administration after SCT
  • \>10.000 EBV DNA copies/ml plasma
  • Recurrence of the haematological disorder needing therapeutic intervention
  • Secondary transplantation
  • SCT with transplant from a haploidentical donor
  • SCT with transplant from umbilical cord blood
  • CD34+-enriched transplant
  • in vitro T-cell depleted transplant
  • Pregnant or breast-feeding female

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Medical Department 5, University Hospital Erlangen

Erlangen, 91054, Germany

RECRUITING

Related Publications (1)

  • Winkler J, Tittlbach H, Schneider A, Vasova I, Strobel J, Herold S, Maas S, Spriewald BM, Repp R, Kordelas L, Mach M, Wolff D, Edinger M, Mackensen A, Winkler TH. Adoptive transfer of donor B lymphocytes: a phase 1/2a study for patients after allogeneic stem cell transplantation. Blood Adv. 2024 May 28;8(10):2373-2383. doi: 10.1182/bloodadvances.2023012305.

MeSH Terms

Conditions

Leukemia, Myeloid, AcutePrecursor Cell Lymphoblastic Leukemia-LymphomaLeukemia, Myelogenous, Chronic, BCR-ABL PositiveLymphoma, Non-HodgkinHodgkin DiseaseMyelodysplastic SyndromesMultiple MyelomaAnemia, Aplastic

Condition Hierarchy (Ancestors)

Leukemia, MyeloidLeukemiaNeoplasms by Histologic TypeNeoplasmsHematologic DiseasesHemic and Lymphatic DiseasesLeukemia, LymphoidLymphoproliferative DisordersLymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesMyeloproliferative DisordersBone Marrow DiseasesChronic DiseaseDisease AttributesPathologic ProcessesPathological Conditions, Signs and SymptomsLymphomaNeoplasms, Plasma CellHemostatic DisordersVascular DiseasesCardiovascular DiseasesParaproteinemiasBlood Protein DisordersHemorrhagic DisordersAnemiaBone Marrow Failure Disorders

Study Officials

  • Julia Winkler, MD

    University Hospital Erlangen

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 21, 2013

First Posted

December 11, 2013

Study Start

November 1, 2013

Primary Completion

December 1, 2015

Study Completion

December 1, 2015

Last Updated

March 26, 2014

Record last verified: 2014-03

Locations