Study of Muscle Repair Following Exercise in Young and Elderly People

NCT02174692 | Completed DMC

• 1 other identifier: MDS
• Study Type: interventional
• Target: 16
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study is to understand how healthy muscle repairs in response to eccentric exercise by evaluating functional, metabolic and molecular measures. Furthermore, this study will aim to understand the repair mechanisms in elderly healthy muscle following eccentric exercise by evaluating the same measures and highlighting age-related differences in repair mechanisms which may account for the age-related loss in muscle mass. It is hypothesised that elderly muscle will have a lesser/ blunted repair process compared to young muscle. To meet the aims of this study and to answer the hypothesis, this study protocol is as follows: Baseline functional, metabolic and molecular values are obtained via functional testing and a muscle biopsy. Participants then perform eccentric exercise designed to induce functional decline and muscle soreness and the contralateral leg performs concentric exercise which isn't designed to induce soreness or functional decline. Using a time-course, various metabolic and molecular measures are taken prior to and up to 7 days post exercise to understand the mechanisms underlying muscle repair. In addition the use of a novel amino acid tracer (D2O) will allow for synthesis measures over the duration of the study rather than the typical tracer techniques which only capture \~12 hours of synthesis.

Conditions

Healthy

Outcome Measures

Primary Outcomes (1)

• Change from baseline in protein synthesis (fractional synthesis rate) in response to eccentric exercise

  Up to one week after exercise

Study Arms (2)

Elderly

EXPERIMENTAL

Exercise One leg eccentric exercise: 7 sets of 10 repetitions at 80% 1 repetition maximum Contralateral leg concentric exercise: 7 sets of 10 repetitions at 80% 1 repetition maximum 2 minutes rest between sets

Other: Exercise

Young

EXPERIMENTAL

Exercise One leg eccentric exercise: 7 sets of 10 repetitions at 80% 1 repetition maximum Contralateral leg concentric exercise: 7 sets of 10 repetitions at 80% 1 repetition maximum 2 minutes rest between sets

Other: Exercise

Interventions

Exercise OTHER

One leg eccentric exercise: 7 sets of 10 repetitions at 80% 1 repetition maximum Contralateral leg concentric exercise: 7 sets of 10 repetitions at 80% 1 repetition maximum 2 mins rest between sets

Elderly; Young

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• kg/m2 BMI
• Male
• Sedentary
• years or 65-75 years

You may not qualify if:

• Active cardiovascular disease
• Cerebrovascular disease including previous stroke or aneurysm
• Respiratory disease including pulmonary hypertension or chronic obstructive pulmonary disease
• Metabolic disease including:
• hyper/ hypo parathyroidism or thyroidism
• Cushing's disease
• Type 1 or 2 diabetes
• Active inflammatory bowel disease
• Renal disease
• Malignancy
• Recent steroid treatment (within 6 months), or hormone replacement therapy
• Clotting dysfunction
• Musculoskeletal or neurological disorders medication that affects muscle metabolism ie. paracetamol or non steroidal anti inflammatories (NSAID's)

Sponsors & Collaborators

• University of Nottingham: lead

Study Sites (1)

MRC-ARUK Centre of Excellence for Musculoskeletal Ageing Research, Division of Metabolic and Molecular Physiology, Postgraduate Entry Medical School, Royal Derby Hospital

Derby, Derbyshire, DE22 3DT, United Kingdom

Location

MeSH Terms

Interventions

Exercise

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: June 11, 2014
• First Posted: June 25, 2014
• Study Start: February 1, 2013
• Primary Completion: March 1, 2015
• Study Completion: March 1, 2015
• Last Updated: May 29, 2015

Record last verified: 2015-05

Locations

GB (1)