MyoGene: Effects of Acute Exercise on (Myokine) Gene Expression in Human Skeletal Muscle

NCT01316731 | Completed

• 1 other identifier: NL33968.081.10
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

Rationale: Proteins released from muscle during and shortly after exercise, often referred to as myokines, may be central to our understanding of the cross-talk during and after exercise between skeletal muscles and other organs, in particular the liver. So far only a few myokines are identified (e.g. IL-6, IL-8, IL-15, TNF-alpha). Taking into account the role of these several known myokines in developing insulin resistance, revealing new putative myokines might provide valuable information and a direction for future research on the pathogenesis and treatment of type 2 diabetes mellitus. Objective: The objective of the present study is to identify novel myokines, expression of which is altered in skeletal muscle after a single bout of exercise. Study design: experimental study. Study population: Ten healthy, male subjects between 40 and 60 years of age and BMI \< 30 kg/m2, will participate in this study. Intervention: A single exercise bout that consists of one hour one-legged cycling on a adapted recumbent cycle ergometer at a submaximal rate. The non-exercising leg will serve as control for the exercising leg. Main study outcomes: Main study outcomes include upregulation of genes in skeletal muscle after exercise (with a focus on genes encoding myokines) and changes of blood plasma levels of selected proteins after exercise.

Conditions

Exercise; Muscle; Gene Expression; Myokine

Outcome Measures

Primary Outcomes (2)

• Gene expression changes in skeletal muscle from baseline to after exercise

  Gene expression is measured using whole genome Affymatrix microarrays Muscle tissue will be collected at time points T = 0 (before exercise) and T = 1 (directly after exercise). Special focus is on the changes in genes coding for myokines. Up to 5 genes will be selected for follow-up analysis.

  T = 0 and T =1

• Changes in plasma levels of selected proteins

  Selected proteins will be analyzed by ELISA assays or western blot analysis, depending on ELISA availability at T = 0, T = 1 and T = 3 (2 hours post-exercise). The selection of the proteins is based on significance, the robustness of induction (\>80% of subjects showing induction) and the magnitude of the induction ( mean fold change \> 2)

  T = 0, T =1 and T = 3

Secondary Outcomes (3)

• PBMC gene expression changes before and after exercise

  T = 0, T = 1 and T =3

• Routine plasma level changes before and after exercise

  T = 0, 1 and 3

• Heart rate changes, baseline compared with exercise

  During the intervention

Study Arms (1)

Exercise

EXPERIMENTAL

Other: Single legged exercise

Interventions

Single legged exercise OTHER

A single exercise bout that consists of one hour one-legged cycling on a adapted recumbent cycle ergometer at a submaximal rate. The non-exercising leg will serve as control for the exercising leg.

Exercise

Eligibility Criteria

• Age: 40 Years - 60 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age 40-60 years
• Male gender
• BMI \< 30 kg/m2

You may not qualify if:

• Exercising regularly (\> 2 times a week, \> 3 hour in total per week)
• Unstable body weight (weight gain or loss \> 3 kg in the past three months)
• Following, or have recently followed a (weight-loss) diet
• Donated or intended to donate blood 2 months before until two months after the study
• Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease)
• Systolic blood pressure \>160 mmHg and/or diastolic blood pressure \>100 mmHg
• Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate).
• Use of antithrombotic therapy (marcoumar, sintromitis).
• Diagnosed diabetes mellitus type 1 or 2.
• Drugs or alcohol abuse ( \> 3 glasses of alcoholic beverages a day).
• (Chronic) injuries of the locomotor system that can interfere with the intervention
• Participated in another study within the last six months

Sponsors & Collaborators

• Wageningen University: lead
• Dutch Diabetes Research Foundation: collaborator

Study Sites (1)

Division of Human Nutrition, Wageningen University

Wageningen, Gelderland, 6703 HA, Netherlands

Location

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 10, 2011
• First Posted: March 16, 2011
• Study Start: February 1, 2011
• Primary Completion: June 1, 2011
• Study Completion: June 1, 2011
• Last Updated: December 21, 2011

Record last verified: 2011-12

Locations

NL (1)