Muscle Response to High Intensity Interval Resistance Training (HIIRT)

NCT04321772 | Completed

• 1 other identifier: HIIRT12
• Study Type: interventional
• Target: 9
• Locations: 1 country
• Sites: 1

Brief Summary

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Conditions

Exercise; Resistance Training

Keywords

resistance training; recovery; muscle hypertrophy; muscle signalling

Outcome Measures

Primary Outcomes (16)

• pAMPKThr172 in skeletal muscle tissue measured by immunoblot

  measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy

  24 hours

• pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot

  measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy

  24 hours

• pS6Ser235/236 in skeletal muscle tissue measured by immunoblot

  measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy

  24 hours

• p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot

  measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy

  24 hours

• ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot

  ACC phosphorylation in skeletal muscle tissue taken by biopsy

  24 hours

• IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  IGF-1 mRNA in skeletal muscle tissue taken by biopsy

  24 hours

• IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  IGF-1ea mRNA in skeletal muscle tissue taken by biopsy

  24 hoursn

• MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  MGF mRNA in skeletal muscle tissue taken by biopsy

  24 hours

• STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  STARS mRNA in skeletal muscle tissue taken by biopsy

  24 hoursn

• Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  Myogenin mRNA in skeletal muscle tissue taken by biopsy

  24 hours

• IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  IL-6 mRNA in skeletal muscle tissue taken by biopsy

  24 hours

• PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  PGC1a4 mRNA in skeletal muscle tissue taken by biopsy

  24 hours

• PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  PGC1a mRNA in skeletal muscle tissue taken by biopsy

  24 hours

• Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  Atrogin mRNA in skeletal muscle tissue taken by biopsy

  24 hours

• Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  Beclin mRNA in skeletal muscle tissue taken by biopsy

  24 hours

• Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

  Myostatin mRNA in skeletal muscle tissue taken by biopsy

  24 hours

Study Arms (2)

High Intensity Interval Resistance Training (HIIRT)

EXPERIMENTAL

HIIRT technique consisted of three sets of: 6 repetitions at 80% 1RM (1 repetition maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets.

Other: High Intensity Interval Resistance Training

Traditional Resistance Training (TRT)

ACTIVE COMPARATOR

TRT protocol performed 3 series of 15 repetitions at 60% 1RM with 75" of rest between sets.

Other: Traditional Resistance Training

Interventions

High Intensity Interval Resistance Training OTHER

Subjects trained one leg with HIIRT

High Intensity Interval Resistance Training (HIIRT)

Traditional Resistance Training OTHER

Subjects trained the other leg with HIIRT

Traditional Resistance Training (TRT)

Eligibility Criteria

• Age: 20 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• moderate active
• experience with resistance training

You may not qualify if:

• history of recent myocardial infarction,
• severe cardiac arrhythmia,
• unstable angina,
• poorly controlled hypertension,
• poorly controlled diabetes mellitus,
• frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Sponsors & Collaborators

• University of Padova: lead
• University of Roma La Sapienza: collaborator

Study Sites (1)

Nutrition and Exercise Lab, DSB, University of Padova

Padua, 35131, Italy

Location

Related Publications (3)

• Paoli A. Resistance training: the multifaceted side of exercise. Am J Physiol Endocrinol Metab. 2012 Feb 1;302(3):E387. doi: 10.1152/ajpendo.00541.2011. No abstract available.

  PMID: 22275762 BACKGROUND

• Paoli A, Moro T, Marcolin G, Neri M, Bianco A, Palma A, Grimaldi K. High-Intensity Interval Resistance Training (HIRT) influences resting energy expenditure and respiratory ratio in non-dieting individuals. J Transl Med. 2012 Nov 24;10:237. doi: 10.1186/1479-5876-10-237.

  PMID: 23176325 BACKGROUND

• Paoli A, Pacelli QF, Cancellara P, Toniolo L, Moro T, Canato M, Miotti D, Reggiani C. Myosin isoforms and contractile properties of single fibers of human Latissimus Dorsi muscle. Biomed Res Int. 2013;2013:249398. doi: 10.1155/2013/249398. Epub 2013 Jul 22.

  PMID: 23971027 BACKGROUND

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Antonio Paoli, MD

  University of Padova

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Masking Details: The samples analysed were masked by a number without any indication of the arm.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Full Professor

Model Details: Same subjects serve as both the test and control arms which allows for within-subject comparison. Using a within-person design, participants trained each lower limb on separate days under two different training stimulus (HIIRT) and traditional training (TRT)

Study Record Dates

• First Submitted: March 19, 2020
• First Posted: March 25, 2020
• Study Start: February 1, 2012
• Primary Completion: May 15, 2012
• Study Completion: February 10, 2013
• Last Updated: March 25, 2020

Record last verified: 2020-03

Data Sharing

• IPD Sharing: Will not share

Locations

IT (1)