Protein Supplementation and Endurance Exercise Adaptations

NCT03462381 | Completed

• 1 other identifier: NL5988508116
• Study Type: interventional
• Target: 44
• Locations: 1 country
• Sites: 1

Brief Summary

Participants will perform three endurance training sessions weekly for 12 weeks. All participants will be randomly assigned to the protein supplementation group or the placebo (iso-caloric carbohydrate) group. During the 12 weeks endurance training program participants allocated to the protein group (30 g protein per beverage) will receive a protein drink after exercise and prior to sleep at night on training days. On non-training days participants will only receive a protein drink before sleep. Participants allocated to the carbohydrate group (30 g carbohydrates) will receive a carbohydrate drink instead of a protein drink.

Conditions

Exercise

Outcome Measures

Primary Outcomes (3)

• Maximal whole-body oxidative capacity

  Maximal oxygen consumption in ml per kg of bodyweight

  Baseline

• Maximal whole-body oxidative capacity

  Maximal oxygen consumption in ml per kg of bodyweight

  6 weeks

• Maximal whole-body oxidative capacity

  Maximal oxygen consumption in ml per kg of bodyweight

  12 weeks

Secondary Outcomes (36)

• Endurance Performance

  Baseline

• Endurance Performance

  6 weeks

• Endurance Performance

  12 weeks

• Maximal activity of citrate synthase

  Baseline

• Maximal activity of citrate synthase

  6 weeks

Study Arms (2)

Protein

EXPERIMENTAL

Three endurance training sessions weekly with protein supplementation post-exercise and before sleep.

Dietary Supplement: Protein

Carbohydrate

PLACEBO COMPARATOR

Three endurance training sessions weekly with carbohydrate supplementation post-exercise and before sleep.

Dietary Supplement: Protein

Interventions

Protein DIETARY_SUPPLEMENT

Three endurance training sessions weekly with protein supplementation post-exercise and before sleep.

Carbohydrate; Protein

Eligibility Criteria

• Age: 18 Years - 30 Years
• Gender Eligibility Details: Young healthy men
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Male
• Age between 18 and 30 years of age
• BMI between 18.5 - 25 kg/m2
• Recreationally active, performing sports on a non-competitive basis
• Maximal wattage of \> 5 watt per kg bodyweight (screening measurement)
• Willing to give muscle biopsies
• Willing to give blood samples
• Having a general-practitioner
• Consumption of alcohol beverages is less than 21 per week
• Able to perform three exercise sessions weekly for 10 weeks

You may not qualify if:

• Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, liver or renal disease, diabetes mellitus type 1 or 2)
• Having a lactose and/or gluten intolerance
• Use of systemic medication (with the exception of antihistaminic medication and the use of occasional painkillers)
• (Chronic) injuries of the locomotor system (e.g. musculoskeletal/orthopedic disorders) that can interfere with the intervention
• Participants who are enrolled in an interventional biomedical research project or have received an investigational new drug or product with the last 30 days prior to screening.
• Smokers and use of illicit drugs
• Blood donor during the study and in the three months before start of the study
• Employed, or intern, or working on a thesis at the department of Human Nutrition at Wageningen University \& Research
• Participating in another scientific study (except EetMeetWeet)

Sponsors & Collaborators

• Wageningen University: lead

Study Sites (1)

Wageningen University & Research

Wageningen, Gelderland, 6708 WE, Netherlands

Location

Related Publications (2)

• Knuiman P, Hangelbroek R, Boekschoten M, Hopman M, Mensink M. Impact of protein supplementation during endurance training on changes in skeletal muscle transcriptome. BMC Genomics. 2020 Jun 9;21(1):397. doi: 10.1186/s12864-020-6686-x.

  PMID: 32517654 DERIVED

• Knuiman P, van Loon LJC, Wouters J, Hopman M, Mensink M. Protein supplementation elicits greater gains in maximal oxygen uptake capacity and stimulates lean mass accretion during prolonged endurance training: a double-blind randomized controlled trial. Am J Clin Nutr. 2019 Aug 1;110(2):508-518. doi: 10.1093/ajcn/nqz093.

  PMID: 31240303 DERIVED

MeSH Terms

Conditions

Motor Activity

Interventions

Proteins

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Amino Acids, Peptides, and Proteins

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Participants will perform three endurance training sessions weekly for 12 weeks. All participants will be randomly assigned to the protein supplementation group or the placebo (iso-caloric carbohydrate) group. During the 12 weeks endurance training program participants allocated to the protein group (30 g protein per beverage) will receive a protein drink after exercise and prior to sleep at night on training days. On non-training days participants will only receive a protein drink before sleep. Participants allocated to the carbohydrate group (30 g carbohydrates) will receive a carbohydrate drink instead of a protein drink.

Study Record Dates

• First Submitted: September 26, 2017
• First Posted: March 12, 2018
• Study Start: August 1, 2017
• Primary Completion: May 28, 2018
• Study Completion: June 1, 2018
• Last Updated: March 20, 2019

Record last verified: 2019-03

Data Sharing

• IPD Sharing: Will not share

Locations

NL (1)