NCT00005908

Brief Summary

This study will assess the usefulness of a technique called complementary deoxyribonucleic acid (cDNA) microarray-an examination of a wide array of genes to identify disease-associated patterns-for measuring tumor response to chemotherapy in breast cancer patients. The study will look for "markers" that can help select the most effective type of chemotherapy. It will also evaluate the safety and effectiveness of a new drug combination of capecitabine and docetaxel. Patients age 18 years and older with stage II or III breast cancer whose tumor is 2 centimeters or larger may be eligible for this study. Those enrolled will be treated with surgery, standard chemotherapy using doxorubicin (Adriamycin) and cyclophosphamide (Cytoxan), and the capecitabine and docetaxel combination. Patients will have a physical examination, mammogram and magnetic resonance imaging to evaluate their tumor before beginning treatment. They will then have four 21-day treatment cycles of docetaxel and capecitabine, as follows: docetaxel intravenously (through a vein) on day 1 and capecitabine pills (by mouth) twice a day from days 2 through 15. No drugs will be given from days 16 through 21. This regimen will be repeated four times, after which the tumor will be re-evaluated by physical examination, mammogram, and magnetic resonance imaging. Patients will then have surgery to remove the cancer-either lumpectomy with removal of the underarm lymph nodes; mastectomy and removal of the underarm lymph nodes; or modified radical mastectomy. After recovery, they will have four more cycles of chemotherapy, this time with a doxorubicin and cyclophosphamide. Both drugs will be given intravenously on day 1 of four 21-day cycles. Some patients who had a mastectomy (depending on their tumor characteristics and whether tumor cells were found in their lymph nodes) and all those who had a lumpectomy will also have radiation therapy. Patients with hormone receptor-positive tumors will also receive tamoxifen treatment for 5 years. In addition to the above procedures, all patients will have tumor biopsies (removal of a small piece of tumor tissue) before beginning treatment, on day 1 of cycle 1, before cycle 2, and at the time of surgery, and physical examinations, chest X-rays, bone scans, computerized tomography (CT) scans, electrocardiograms, multi-gated acquisition scan-MUGA (nuclear medicine test of cardiac function) or echocardiograms of heart function, mammograms and blood tests at various times during the study. Patients will be followed at National Institutes of Health (NIH) for 3 years after diagnosis with physical examinations, blood tests, X-rays, and computed tomography (CT) scans. Although it is not known whether this treatment will help an individual patient's cancer, possible benefits are tumor shrinkage and decreased risk of disease recurrence. In addition, the information gained about genetic changes after chemotherapy will help determine if additional studies on the use of cDNA microarray to measure tumor response are warranted.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
30

participants targeted

Target at below P25 for phase_2 breast-cancer

Timeline
Completed

Started Jun 2000

Longer than P75 for phase_2 breast-cancer

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 1, 2000

Completed
12 days until next milestone

First Submitted

Initial submission to the registry

June 13, 2000

Completed
1 day until next milestone

First Posted

Study publicly available on registry

June 14, 2000

Completed
7.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 1, 2008

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 1, 2008

Completed
4.7 years until next milestone

Results Posted

Study results publicly available

August 27, 2012

Completed
Last Updated

March 19, 2013

Status Verified

March 1, 2013

Enrollment Period

7.6 years

First QC Date

June 13, 2000

Results QC Date

February 14, 2012

Last Update Submit

March 13, 2013

Conditions

Keywords

cDNA MicroarrayStage II and Stage III Breast CancerBiological ResponseMolecular ProfilingFine Needle AspirateBreast Cancer

Outcome Measures

Primary Outcomes (4)

  • Number of Participants With Adverse Events

    Here is the number of participants with adverse events. For the detailed list of adverse events see the adverse event module.

    6 years

  • Overall Clinical Response Rate

    Overall response rate is defined as the percentage of participants with a CR (complete disappearance of all target lesions), PR (a 30% decrease in the sum of the longest diameter of target lesions) determined by clinical measurements per the Response Evaluation Criteria in Solid Tumors (RECIST) and/or a complete pathologic response (disappearance of all invasive tumor pathologically or presence of ductal carcinoma in situ) per the Chevallier criteria. For details about the RECIST or Chevallier criteria see the protocol link module.

    6 years

  • Complementary Deoxyribonucleic Acid (cDNA) Expression

    Patients were classified as responders or non-responders based on change in tumor size by clinical exam and pathologic response.For instance, patients with a pathological complete response, micro-invasive disease at surgery, or clinical complete response after four cycles of treatment were considered responders. Changes in gene expression associated with treatment was assessed before/after chemotherapy. All gene expression summary intensities below 50 were thresholded to the value of 50 and genes showing variability significantly smaller than the median gene variability were screened out.

    6 years

  • Number of Participants, e.g. Responders and Non-responders With a Percent Change in Expression Patterns After Chemotherapy With Changes in Expression Patterns After Chemotherapy in Preclinical Models

    Patients were classified as responders or non-responders based on change in tumor size by clinical exam and pathologic response.For instance, patients with a pathological complete response, micro-invasive disease at surgery, or clinical complete response after four cycles of treatment were considered responders. Changes in gene expression associated with treatment was assessed before/after chemotherapy. All gene expression summary intensities below 50 were thresholded to the value of 50 and genes showing variability significantly smaller than the median gene variability were screened out.

    6 years

Study Arms (2)

Dose A-Cohort 1-Arm 1-Docetaxel & Capecitabine

EXPERIMENTAL

Docetaxel 75 mg/m\^2 intravenous day 1, capecitabine 1000 mg/m\^2 orally twice daily day 2-15 for 4 cycles

Drug: Docetaxel - Dose ADrug: AnastrozoleDrug: cyclophosphamideDrug: Doxorubicin hydrochlorideDrug: Tamoxifen CitrateDrug: Capecitabine - Dose A

Dose B-Cohort 2-Arm 2 Reduced dose-Docetaxel & Capecitabine

EXPERIMENTAL

Docetaxel 60 mg/m\^2 intravenous day 1, capecitabine 937.5 mg/m\^2 orally twice daily day 2-15 for 4 cycles

Drug: AnastrozoleDrug: cyclophosphamideDrug: Docetaxel - Dose BDrug: Doxorubicin hydrochlorideDrug: Tamoxifen CitrateDrug: Capecitabine - Dose B

Interventions

Dose A-Cohort 1 Docetaxel 75 mg/m\^2 intravenous day 1

Also known as: Taxotere/Xeloda
Dose A-Cohort 1-Arm 1-Docetaxel & Capecitabine

1 mg orally daily for five years

Also known as: Arimidex
Dose A-Cohort 1-Arm 1-Docetaxel & CapecitabineDose B-Cohort 2-Arm 2 Reduced dose-Docetaxel & Capecitabine

600 mg/m\^2 will be diluted in 100 mL 0.9% normal saline (NS) and administered intravenously over 30 minutes on day 1

Also known as: Cytoxan
Dose A-Cohort 1-Arm 1-Docetaxel & CapecitabineDose B-Cohort 2-Arm 2 Reduced dose-Docetaxel & Capecitabine

Dose B - Cohort 2 Docetaxel 60 mg/m\^2 intravenous day 1

Also known as: Taxotere
Dose B-Cohort 2-Arm 2 Reduced dose-Docetaxel & Capecitabine

60 mg/m\^2 will be administered as a slow intravenous push on day 1

Also known as: Adriamycin
Dose A-Cohort 1-Arm 1-Docetaxel & CapecitabineDose B-Cohort 2-Arm 2 Reduced dose-Docetaxel & Capecitabine

20 mg/day orally for five years

Also known as: Nolvadex
Dose A-Cohort 1-Arm 1-Docetaxel & CapecitabineDose B-Cohort 2-Arm 2 Reduced dose-Docetaxel & Capecitabine

Dose B - Cohort 2 capecitabine 937.5 mg/m\^2 orally twice daily day 2-15

Dose B-Cohort 2-Arm 2 Reduced dose-Docetaxel & Capecitabine

capecitabine 1000 mg/m\^2 orally twice daily day 2-15 for 4 cycles

Dose A-Cohort 1-Arm 1-Docetaxel & Capecitabine

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Stage II or III breast cancer with a tumor size of greater than 2 cm. Patients with a previous biopsy are eligible provided adequate tumor tissue remains for biopsy in this study.
  • At least 18 years of age.
  • Adequate hematopoietic function as defined by absolute neutrophil count greater than 1200/mm\^3 and platelet count greater than 100,000/mm\^3.
  • Adequate renal function as defined by creatinine less than 1.6 mg/dL.
  • Adequate hepatic function as defined by total (T.) bilirubin less than 1.4 mg/dL and serum glutamic oxaloacetic transaminase (SGOT)/serum glutamic pyruvic transaminase (SGPT) less than 1.5 times the upper limit of normal and alkaline phosphatase less than 2.5 times upper limit of normal
  • Zubrod Performance status 0-2.

You may not qualify if:

  • Medical or psychiatric condition that, in the opinion of the Principal Investigator, would preclude chemotherapy administration. Patients may be evaluated by psychiatry or medical subspecialties as appropriate.
  • Pregnant or lactating women
  • Known bleeding disorders
  • Hypersensitivity to Tween 80 (Polysorbate)
  • Cardiac ejection fraction below normal limits, myocardial infarction within the past 12 months, or symptomatic arrhythmia requiring medical intervention.
  • Prior chemotherapy or hormonal therapy for breast cancer. Patients treated with hormonal chemoprevention (tamoxifen or raloxifene) will be eligible.
  • Active malignancy diagnosed within the last 5 years. (Cervical cancer or non-melanomatous skin cancer that has been treated with curative intent will be eligible).

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

National Naval Medical Center

Bethesda, Maryland, 20889, United States

Location

Related Publications (5)

  • Elledge RM, Gray R, Mansour E, Yu Y, Clark GM, Ravdin P, Osborne CK, Gilchrist K, Davidson NE, Robert N, et al. Accumulation of p53 protein as a possible predictor of response to adjuvant combination chemotherapy with cyclophosphamide, methotrexate, fluorouracil, and prednisone for breast cancer. J Natl Cancer Inst. 1995 Aug 16;87(16):1254-6. doi: 10.1093/jnci/87.16.1254. No abstract available.

    PMID: 7563172BACKGROUND
  • MacGrogan G, Mauriac L, Durand M, Bonichon F, Trojani M, de Mascarel I, Coindre JM. Primary chemotherapy in breast invasive carcinoma: predictive value of the immunohistochemical detection of hormonal receptors, p53, c-erbB-2, MiB1, pS2 and GST pi. Br J Cancer. 1996 Nov;74(9):1458-65. doi: 10.1038/bjc.1996.565.

    PMID: 8912545BACKGROUND
  • Harvey JM, Clark GM, Osborne CK, Allred DC. Estrogen receptor status by immunohistochemistry is superior to the ligand-binding assay for predicting response to adjuvant endocrine therapy in breast cancer. J Clin Oncol. 1999 May;17(5):1474-81. doi: 10.1200/JCO.1999.17.5.1474.

    PMID: 10334533BACKGROUND
  • Lebowitz PF, Eng-Wong J, Swain SM, Berman A, Merino MJ, Chow CK, Venzon D, Zia F, Danforth D, Liu E, Zujewski J. A phase II trial of neoadjuvant docetaxel and capecitabine for locally advanced breast cancer. Clin Cancer Res. 2004 Oct 15;10(20):6764-9. doi: 10.1158/1078-0432.CCR-04-0976.

  • Korde LA, Lusa L, McShane L, Lebowitz PF, Lukes L, Camphausen K, Parker JS, Swain SM, Hunter K, Zujewski JA. Gene expression pathway analysis to predict response to neoadjuvant docetaxel and capecitabine for breast cancer. Breast Cancer Res Treat. 2010 Feb;119(3):685-99. doi: 10.1007/s10549-009-0651-3.

Related Links

MeSH Terms

Conditions

Breast Neoplasms

Interventions

DocetaxelCapecitabineAnastrozoleCyclophosphamideDoxorubicinTamoxifen

Condition Hierarchy (Ancestors)

Neoplasms by SiteNeoplasmsBreast DiseasesSkin DiseasesSkin and Connective Tissue Diseases

Intervention Hierarchy (Ancestors)

TaxoidsCyclodecanesCycloparaffinsHydrocarbons, AlicyclicHydrocarbons, CyclicHydrocarbonsOrganic ChemicalsDiterpenesTerpenesDeoxycytidineCytidinePyrimidine NucleosidesPyrimidinesHeterocyclic Compounds, 1-RingHeterocyclic CompoundsFluorouracilUracilPyrimidinonesDeoxyribonucleosidesNucleosidesNucleic Acids, Nucleotides, and NucleosidesNitrilesTriazolesAzolesPhosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedPhosphoramidesOrganophosphorus CompoundsDaunorubicinAnthracyclinesNaphthacenesPolycyclic Aromatic HydrocarbonsHydrocarbons, AromaticPolycyclic CompoundsAminoglycosidesGlycosidesCarbohydratesStilbenesBenzylidene CompoundsBenzene Derivatives

Results Point of Contact

Title
JoAnne Zujewski, M.D.
Organization
National Cancer Institute, National Institutes of Health

Study Officials

  • JoAnne Zujewski, M.D.

    National Cancer Institute (NCI), National Institutes of Health (NIH)

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
NIH
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

June 13, 2000

First Posted

June 14, 2000

Study Start

June 1, 2000

Primary Completion

January 1, 2008

Study Completion

January 1, 2008

Last Updated

March 19, 2013

Results First Posted

August 27, 2012

Record last verified: 2013-03

Locations