Grape Seed Extract Supplementation on Cycling Time-to-Exhaustion Performance.

NCT07867977 | Completed

• 1 other identifier: CMUH111-REC3-160
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

To address the physical fatigue and muscle recovery demands in endurance exercise, this study evaluates Grape Seed Extract (GSE) as a potential ergogenic aid. The research aims to investigate whether short-term, high-dose GSE supplementation (1,800 mg/day for 7 days) prolongs cycling time-to-exhaustion (TTE) at 75% VO2max while attenuating exercise-induced muscle damage, metabolic stress, and mood disturbance in collegiate athletes. This study seeks to enhance high-intensity endurance performance and support the development of functional botanical anti-fatigue supplements.

Conditions

Healhty

Keywords

exercise performance; muscle damage; fatigue

Outcome Measures

Primary Outcomes (1)

• Cycling Time-to-Exhaustion (TTE)

  Participants performed an exhaustive cycling exercise at 75% VO2max, and speed was maintained at 60 rpm on a stationary ergometer bike, where the individual maximum TTE was recorded.

  At baseline (pre-supplementation) and after 7 days of GSE supplementation (post-supplementation)

Secondary Outcomes (10)

• Serum Creatine Kinase (CK) Concentration

  Assessed at Day 8 (pre-intervention) and Day 43 (post-intervention) at four time points on each experimental day: fasting baseline (07:00), pre-exercise (after 1-hour rest), 30 minutes during cycling exercise, and immediately at exhaustion.

• Lactate Dehydrogenase (LDH) Activity

  Assessed at Day 8 (pre-intervention) and Day 43 (post-intervention) at four time points on each experimental day : fasting baseline (07:00), pre-exercise (after 1-hour rest), 30 minutes during cycling exercise, and immediately at exhaustion.

• Uric Acid (UA) Levels

  Assessed at Day 8 (pre-intervention) and Day 43 (post-intervention) at four time points on each experimental day: fasting baseline (07:00), pre-exercise (after 1-hour rest), 30 minutes during cycling exercise, and immediately at exhaustion.

• Profile of Mood States (POMS) - Vigor Subscale Score

  Assessments were conducted on Day 8 (pre-intervention) and Day 43 (post-intervention), immediately following the time-to-exhaustion (TTE) test.

• Profile of Mood States (POMS) - Self-esteem Subscale Score

  Assessments were conducted on Day 8 (pre-intervention) and Day 43 (post-intervention), immediately following the time-to-exhaustion (TTE) test.

Study Arms (1)

GSE Supplementation Group

EXPERIMENTAL

To approximate that cumulative exposure over a shorter period, Participants were oral 1,800 mg/day of GSE for 7 consecutive days.

Dietary Supplement: Grape seed extract

Interventions

Grape seed extract DIETARY_SUPPLEMENT

Participants were oral 1,800 mg/day of GSE for 7 consecutive days.

GSE Supplementation Group

Eligibility Criteria

• Age: 20 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Capable of completing a maximal oxygen uptake (VO2max) test.

You may not qualify if:

• diabetes mellitus, respiratory diseases, heart diseases, coagulopathy, hematoma, or other medical illnesses, as well as individuals unable to perform the cycling test to exhaustion or having a fear of blood sampling.

Sponsors & Collaborators

• China Medical University Hospital: lead

Study Sites (1)

China Medical University

Taichung, Taiwan

Location

MeSH Terms

Conditions

Fatigue

Interventions

Grape Seed Extract

Condition Hierarchy (Ancestors)

Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Plant Extracts; Plant Preparations; Biological Products; Complex Mixtures; Pharmaceutical Preparations

Study Officials

• Jung-Piao Tsao

  China Medical University, China

  STUDY CHAIR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: OTHER
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: The study employed a single-group, pretest-posttest repeated-measures intervention design. For the preliminary test, maximal oxygen uptake (VO2max) was assessed seven days before the pre-experiment. The pre- and post-intervention experiments were separated by a 28-day interval.

Study Record Dates

• First Submitted: September 14, 2026
• First Posted: October 9, 2026
• Study Start: January 9, 2023
• Primary Completion: July 27, 2023
• Study Completion: July 27, 2023
• Last Updated: October 9, 2026

Record last verified: 2026-10

Data Sharing

• IPD Sharing: Will not share

Locations

TW (1)