Effect of Non-surgical Treatment of Periodontitis Patients Focusing on Inflammatory Mediators in Gingival Crevicular Fluid

NCT07503041 | Completed

• 2 other identifiers: REK 201716502017/1650/REK Nord
• Study Type: observational
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

This study investigates inflammatory biomarkers in gingival crevicular fluid (GCF) from smokers and non-smokers with severe periodontitis, before and during non-surgical periodontal therapy (NSPT). During periodontal disease development the periodontal pockets become deeper. In the inflamed periodontal tissue the interstitial fluid is filtered out into the periodontal pocket. The GCF reflects local inflammatory activity and contains cytokines/chemokines that may serve as biomarkers for disease severity and treatment response. Investigators aim at collecting GCF from periodontal pockets from each patients before and during NSPT. Investigators compare non-smokers and smokers. The samples will be analysed for pro-inflammatory mediators and investigators aim at biomarkers that are at a high level in deep inflamed periodontal pockets. Investigators also want to observe if these biomarkers are altered in GCF after periodontal therapy. Finally investigators want to compare non-smokers and smokers with respect to treatment outcome and respons of chemokines/cytokines in GCF.

Conditions

Periodontitis Chronic Generalized Severe; Periodontitis

Keywords

Gingival crevicular fluid; periodontal pocket; chemokines; inflammation

Outcome Measures

Primary Outcomes (1)

• Biomarker measurement in GCF

  Analyse the GCF for 40 biomarkers

  Within a period of 5 years

Study Arms (2)

Collecting gingival crevicular fluid

Applying a paperpoint in a periodontal pocket for 30 sec

Other: Collecting fluid for biomarker analyses; Procedure: Hygiene instruction, tooth cleaning, plaque removal

Treatment of periodontitis

Non surgical treatment of periodontitis patients

Other: Collecting fluid for biomarker analyses; Procedure: Hygiene instruction, tooth cleaning, plaque removal

Interventions

Collecting fluid for biomarker analyses OTHER

We are looking at spesific biomarkers in GCF that has not been investigated before

Collecting gingival crevicular fluid; Treatment of periodontitis

Hygiene instruction, tooth cleaning, plaque removal PROCEDURE

Collecting gingival crevicular fluid; Treatment of periodontitis

Eligibility Criteria

• Age: 16 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Patients with severe periodontitis referred to our specialist clinic

Sponsors & Collaborators

• Oral Health Centre of Expertise in Western Norway: lead

Study Sites (1)

Postboks 2354 Møllendal

Bergen, 5867, Norway

Location

MeSH Terms

Conditions

Periodontitis; Periodontal Pocket; Inflammation

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: February 5, 2026
• First Posted: March 31, 2026
• Study Start: January 8, 2016
• Primary Completion: December 21, 2025
• Study Completion: December 21, 2025
• Last Updated: March 31, 2026

Record last verified: 2026-03

Locations

NO (1)