A Pilot Study on Skeletal Muscle Proteome Dynamics in Younger and Older Men Undertaking Normal Activity or Resistance Exercise Training.

NCT07473297 | Completed

• 1 other identifier: 22/SPS/075
• Study Type: interventional
• Target: 8
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this basic research study is to investigate effect of resistance exercise on the quality of muscle proteins in younger and older men. The main question\[s\] it aims to answer \[is/are\]: whether there is a difference in muscle protein quality between younger and older adults and if resistance exercise training has a beneficial impact on the quality of muscle proteins? Participants will perform five bouts of unilateral leg press exercise during a 14 day period of deuterium oxide consumption and biopsy samples will be taken from both their exercised leg and non-exercised leg before and after the intervention period to enable the measurement of muscle protein abundances and turnover rates.

Conditions

Ageing Well

Keywords

Liverpool Healthy Muscle Ageing Pilot

Outcome Measures

Primary Outcomes (1)

• Muscle proteome dynamics

  Assessment of muscle proteome quality using dynamic proteome profiling to measure the abundances and turnover rates of muscle proteins.

  Throughout the 14 day intervention period of unilateral leg press exercise.

Study Arms (1)

Unilateral resistance exercise training

OTHER

Unilateral resistance exercise of the dominant leg using leg press exercise

Other: Resistance Exercise

Interventions

Resistance Exercise OTHER

Unilateral leg press. Participants undertook a supervised training session of unilateral leg-press exercise at a standardized time of day. During each session, participants performed a warm-up consisting of 5 repetitions at 70 % of 10RM. Participants performed three sets of 10 repetitions at a lifting intensity of 90 % of 10 RM with 2- to 3-min rest between sets.

Unilateral resistance exercise training

Eligibility Criteria

• Age: 20 Years - 75 Years
• Gender Eligibility Details: Male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Male
• Age 25-35 OR 65-75 years

You may not qualify if:

• female
• take part in \>3 bouts of endurance/resistance based training per week
• are pregnant
• have any previous diagnosis of ischaemic heart disease, diabetes mellitus myopathy or any neuromuscular disorder.
• take any anticoagulant medications such as warfarin
• have a current musculoskeletal injury.
• currently unwell with cold or flu
• have a chronic connective tissue disorder
• have a cardiovascular disease
• are currently enrolled in any other research study

Sponsors & Collaborators

• Liverpool John Moores University: lead
• Biotechnology and Biological Sciences Research Council: collaborator

Study Sites (1)

Liverpool John Moores University

Liverpool, Merseyside, L3 3AF, United Kingdom

Location

MeSH Terms

Interventions

Resistance Training

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor of Muscle Proteomics

Model Details: Unilateral resistance exercise training

Study Record Dates

• First Submitted: March 3, 2026
• First Posted: March 16, 2026
• Study Start: March 10, 2023
• Primary Completion: March 12, 2023
• Study Completion: August 28, 2025
• Last Updated: March 16, 2026

Record last verified: 2026-03

Data Sharing

• IPD Sharing: Will not share

Locations

GB (1)