NCT07473193

Brief Summary

This randomized, double-blind, placebo-controlled crossover trial aims to evaluate the acute effects of a nitrate-rich beetroot-based beverage on repeated sprint performance in trained athletes. Ten athletes will complete two experimental conditions in randomized order: beetroot beverage (BET) and placebo (PLA), separated by a 7-day washout period. The beetroot beverage consists of 200 mL of a mixture containing beetroot, cocoa, and green tea, providing approximately 6.4 mmol of nitrate. In each condition, participants will perform a Running Anaerobic Sprint Test (RAST), consisting of six maximal 35-meter sprints with 10 seconds of passive recovery between sprints. The primary outcome is mean power output across the six sprints. Secondary outcomes include fatigue index, sprint-by-sprint power output, heart rate, rating of perceived exertion (Borg 6-20 scale), maximal oxygen uptake (VO₂max), and sleep quality assessed using the Insomnia Severity Index (ISI).

Trial Health

35
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
10

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Mar 2026

Shorter than P25 for not_applicable

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

February 15, 2026

Completed
25 days until next milestone

Study Start

First participant enrolled

March 12, 2026

Completed
4 days until next milestone

First Posted

Study publicly available on registry

March 16, 2026

Completed
27 days until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 12, 2026

Completed
18 days until next milestone

Study Completion

Last participant's last visit for all outcomes

April 30, 2026

Completed
Last Updated

March 16, 2026

Status Verified

March 1, 2026

Enrollment Period

1 month

First QC Date

February 15, 2026

Last Update Submit

March 10, 2026

Conditions

Keywords

Beetroot juice

Outcome Measures

Primary Outcomes (1)

  • Mean power output (W) during six maximal 35-m sprints

    Mean power output will be calculated from six maximal 35-m sprint efforts separated by standardized recovery intervals. Sprint time will be recorded and converted to power output (W). The mean value across all six sprints will be used as the outcome measure.

    Baseline and immediately post-intervention.

Study Arms (2)

Beetroot Beverage (BET)

EXPERIMENTAL

Participants consumed 200 mL of a beetroot-based antioxidant beverage containing beetroot, cocoa, and green tea extract, providing approximately 6.4 mmol of dietary nitrate. The beverage was ingested 2-3 hours before performing the exercise test.

Dietary Supplement: Beetroot-based antioxidant beverage

Placebo Beverage (PLA)

PLACEBO COMPARATOR

Participants consumed 200 mL of a placebo beverage matched for color, taste, and texture but without nitrate or active antioxidant compounds. The beverage was ingested 2-3 hours before performing the exercise test.

Dietary Supplement: Beetroot-based antioxidant beverage

Interventions

Participants consumed 200 mL of a beetroot-based antioxidant beverage containing beetroot, cocoa, and green tea extract, providing approximately 6.4 mmol of dietary nitrate. The beverage was administered 2-3 hours before performing a Running Anaerobic Sprint Test (RAST). The formulation was designed to deliver nitrate and polyphenols with potential ergogenic and antioxidant effects. A 7-day washout period separated crossover conditions.

Beetroot Beverage (BET)Placebo Beverage (PLA)

Eligibility Criteria

Age18 Years - 35 Years
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy trained athletes
  • Age between 18-35 years
  • Regular training ≥3 times/week
  • No cardiovascular or metabolic disease

You may not qualify if:

  • Age between 18-35 years
  • Regular training ≥3 times/week
  • No cardiovascular or metabolic disease

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Publications (1)

  • Ponce Dávila, E. O., Neder Suárez, D., Muñoz Daw, M. de J., Carrasco Legleu, C. E., & Benítez Hernández, Z. P. (2024). Diseño y análisis de una bebida deportiva a base de betabel. Revista Mexicana De Ciencias De La Cultura Física, 3(7), 1-10. https://doi.org/10.54167/rmccf.v3i7.1370

    RESULT

Central Study Contacts

Emmanuel Omar Ponce Dávila, MC

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
Masking Details
The study was conducted under a double-blind design. Both participants and investigators were unaware of the beverage allocation (beetroot-based beverage or placebo). The beverages were matched in volume (200 mL), color, taste, and texture. Allocation was performed by an independent researcher not involved in data collection or analysis. Outcome assessors and data analysts were blinded to treatment order until completion of statistical analyses.
Purpose
OTHER
Intervention Model
CROSSOVER
Model Details: Randomized, double-blind, placebo-controlled crossover design. Participants completed two experimental trials (beetroot-based beverage and placebo) separated by a 7-day washout period. The order of interventions was randomized. Each participant served as his own control. Performance outcomes were assessed using the Running Anaerobic Sprint Test (RAST).
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

February 15, 2026

First Posted

March 16, 2026

Study Start

March 12, 2026

Primary Completion

April 12, 2026

Study Completion

April 30, 2026

Last Updated

March 16, 2026

Record last verified: 2026-03

Data Sharing

IPD Sharing
Will not share