Beetroot Supplementation and High Intensity Exercise

NCT05841407 | Completed

• 1 other identifier: IRB-2022-880
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

Ingested beetroot contains inorganic nitrate which is later converted to nitric oxide (NO) in the bloodstream. NO is an important signaling molecule with several physiological functions in the body including acting as a vasodilator, expanding arteries and improving blood flow. Beetroot juice contain inorganic nitrate and consumption of beetroot has been shown to improve skeletal muscle contractility and metabolism enhancing high-intensity exercise performance. Isotonic sports drinks contain carbohydrates and sodium, which also improve exercise performance by providing exogenous glucose for energy to the working muscle and central nervous system and enabling faster absorption of fluid into the bloodstream. The ingestion of exogenous nitrate and isotonic-carbohydrate drink are recognized strategies for promoting exercise performance but whether they can be effectively combined has not been shown. This study will compare the salivary nitrite response, muscle oxygenation and time-trial performance after ingestion of two isotonic drinks with high in nitrate or with no nitrate.

Conditions

Exercise Performance

Outcome Measures

Primary Outcomes (1)

• 10 km cycling time trial (endurance) performance

  Time trial

  1 hour after drink ingestion the time taken to complete 10 km on a stationary cycle ergometer will be assessed. Time to complete this is the primary outcome measure .

Secondary Outcomes (2)

• Salivary nitrate

  Four samples over 2 hours

• Near infra-red spectroscopy (NIRS)

  During cycling ~1 hour

Study Arms (2)

High nitrate

EXPERIMENTAL

High nitrate isotonic drink

Dietary Supplement: High nitrate isotonic drink

No nitrate

ACTIVE COMPARATOR

No nitrate isotonic drink

Dietary Supplement: No nitrate isotonic drink

Interventions

High nitrate isotonic drink DIETARY_SUPPLEMENT

High nitrate (12.9 mmol) drink with carbohydrate and minerals

High nitrate

No nitrate isotonic drink DIETARY_SUPPLEMENT

Carbohydrate and mineral isotonic drink

No nitrate

Eligibility Criteria

• Age: 21 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age: 21 - 40 years
• Healthy and injury-free (e.g., no cardiovascular disease, diabetes mellitus, orthopaedic impairment that interferes with moderate-to-vigorous exercise)
• Blood pressure \< 130/90 mmHg
• Physically active: Engaging in a minimum of 150 minutes of moderate-intensity exercise or 75 minutes of vigorous-intensity physical activity each week.
• Recreational cyclist (Minimum 1 hour cycling per week)
• Body mass index: 18.5-25 kg/m2
• Non-smoker
• Asian ethnicity by self identification

You may not qualify if:

• Any allergies to beetroot or isotonic drink.
• Asthma.
• Any report of personal health issues that may compromise the individual's ability to undertake exercise safely.
• Any recent Covid vaccination (within last 2 weeks).

Sponsors & Collaborators

• Nanyang Technological University: lead

Study Sites (1)

Human Bioenergetics Laboratory, National Institute of Education, Nanyang Technological University

Singapore, 637616, Singapore

Location

Study Officials

• Stephen Burns, PhD

  Nanyang Technological University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Masking Details: Both isotonic drinks (high nitrate or no nitrate) are deidentified and labelled (coded) by a third party not involved with the research. Randomisation is completed by a third party not involved in the testing.
• Purpose: OTHER
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: April 11, 2023
• First Posted: May 3, 2023
• Study Start: March 15, 2023
• Primary Completion: March 14, 2024
• Study Completion: March 14, 2024
• Last Updated: April 9, 2024

Record last verified: 2024-04

Data Sharing

• IPD Sharing: Will share
• Shared Documents: ANALYTIC CODE
• Time Frame: Upon publication - permanent repository
• Access Criteria: Public repository - exact web address available upon publication.

Locations

SG (1)