Effect of β-alanine Supplementation and Training on Ergogenic Measures and Plasma Metabolites in Untrained Young Adults

NCT07404540 | Completed

• 1 other identifier: 316-2406-1/KK15/2011
• Study Type: interventional
• Target: 43
• Locations: 1 country
• Sites: 1

Brief Summary

This study enrolled untrained young adults to evaluate the effects of β-alanine supplementation combined with a structured exercise training program. Participants were randomly assigned to receive either β-alanine or a placebo while completing a six-week training regimen. Before and after the intervention, physical performance tests and blood samples were collected to assess changes in performance measures and biochemical markers in the blood. The purpose of the study was to determine how β-alanine supplementation influences exercise performance and related physiological outcomes in individuals who had not previously engaged in regular training.

Conditions

Dietary Supplementation; Healthy Vollunteer

Keywords

β-alanine; Exercise training; Dietary supplementation; Physical performance; Untrained adults

Outcome Measures

Primary Outcomes (2)

• Change in maximal muscle strength

  Maximal muscle strength was assessed by dynamometric measurements of maximal voluntary isometric contraction.

  Baseline and 6 weeks

• Change in aerobic capacity

  Aerobic capacity was estimated by VO₂max derived from the 20-m multistage fitness test (shuttle run).

  Baseline and 6 weeks

Secondary Outcomes (7)

• Body mass index (BMI)

  Baseline and 6 weeks

• Body fat percentage

  Baseline and 6 weeks

• Forced vital capacity (FVC)

  Baseline and 6 weeks

• Acute blood lactate response to dynamometric exercise

  Baseline and 6 weeks

• Plasma β-alanine concentration

  Baseline and 6 weeks

Other Outcomes (1)

• Adverse events

  Up to 6 weeks

Study Arms (2)

β-Alanine Supplementation

EXPERIMENTAL

Participants received β-alanine supplementation at a dose of 50 mg/kg/day, divided into multiple daily doses, in combination with a supervised six-week exercise training program.

Dietary Supplement: β-Alanine

Placebo

PLACEBO COMPARATOR

Participants received a visually identical placebo following the same dosing schedule as the supplementation group, in combination with a supervised six-week exercise training program.

Dietary Supplement: Placebo

Interventions

β-Alanine DIETARY_SUPPLEMENT

β-alanine was administered orally at a dose of 50 mg/kg/day, divided into multiple daily doses and consumed with meals, throughout the six-week training intervention.

β-Alanine Supplementation

Placebo DIETARY_SUPPLEMENT

Participants received visually identical placebo capsules following the same dosing schedule as the β-alanine group during the six-week training intervention.

Placebo

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy young adults aged 18-30 years.
• Untrained individuals without participation in structured resistance or endurance training during the previous 6 months.
• Not physically inactive, but not engaged in regular structured exercise training.
• Ability to participate in a six-week supervised multicomponent exercise training program.
• Willingness to comply with study procedures, training sessions, and supplementation protocol.
• Provision of written informed consent.

You may not qualify if:

• Known cardiovascular, metabolic, neuromuscular, or musculoskeletal disease.
• Acute injury at the time of enrollment.
• Regular use of medications or dietary supplements affecting muscle metabolism or exercise performance.
• Participation in structured resistance or endurance training during the previous 6 months.
• Failure to comply with the study protocol or training requirements.

Sponsors & Collaborators

• University of Pecs: lead

Study Sites (1)

University of Pécs

Pécs, Hungary

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Model Details: Participants were randomly assigned in a 1:1 ratio to receive either β-alanine supplementation or a matchin

Study Record Dates

• First Submitted: January 29, 2026
• First Posted: February 11, 2026
• Study Start: January 1, 2012
• Primary Completion: March 1, 2012
• Study Completion: March 1, 2012
• Last Updated: February 11, 2026

Record last verified: 2026-02

Data Sharing

• IPD Sharing: Will not share

Locations

HU (1)