Effects of E-Cigarette Use on Oxidative Stress and Matrix Degradation

NCT07338318 | Completed

• 2 other identifiers: AnkaraMedipolUni.16.04.2025/80
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

This cross-sectional observational study aims to investigate the effects of e-cigarette use on oxidative and matrix-degrading pathways in periodontally healthy individuals. Gingival crevicular fluid biomarkers, including matrix metalloproteinase-9 (MMP-9), reactive oxygen species (ROS), and forkhead-box-1 (FOXO1), will be analyzed to compare the biochemical and inflammatory responses among three groups: e-cigarette users, conventional cigarette smokers, and non-smokers. The study seeks to clarify whether e-cigarette use induces subclinical inflammatory or proteolytic activity in oral tissues comparable to that of traditional smoking.

Conditions

Smoking Behaviors; Periodontal Health

Keywords

e-cigarette; periodontal health; matrix metalloproteinaz-9; forkhead-box protein 1

Outcome Measures

Primary Outcomes (1)

• (MMP-9, ROS, FOXO-1) Concentration in GCF

  MMP-9, ROS, FOXO-1 levels will be quantified using a commercially available enzyme-linked immunosorbent assay (ELISA) to assess proteolytic activity in periodontally healthy individuals. The mean MMP-9, ROS, and FOXO-1 concentrations (ng/mL) will be compared among three groups: exclusive e-cigarette users, conventional smokers, and non-smokers.

  Single measurement at baseline (cross-sectional assessment)

Secondary Outcomes (1)

• correlation clinical parameters and gcf biomarkers

  single time point

Study Arms (1)

E-Cigarette Users

Participants who have used electronic cigarettes exclusively for at least 12 months, with no history of conventional cigarette use during that period. Eligible individuals must report daily vaping habits and nicotine-containing e-liquid use. All participants will present with clinically healthy periodontal tissues,

Other: E-Cigarette Use

Interventions

E-Cigarette Use OTHER

Exclusive e-cigarette users for at least 12 consecutive months, with no conventional cigarette or other tobacco product use during that period. Participants report daily vaping of nicotine-containing e-liquids using refillable or disposable e-cigarette devices. The group represents chronic exposure to aerosolized nicotine and flavoring agents without combustion products

E-Cigarette Users

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Systemically and periodontally healthy smokers, e-cigarette users, and non-smoking individuals over 18 years old.

You may qualify if:

• E-cigarette using, conventional smokers, non-smokers

You may not qualify if:

• Having active periodontal disease, systemic health problems

Sponsors & Collaborators

• Ankara Medipol University: lead

Study Sites (1)

Ankara Medipol Uni.

Ankara, Ankara, 06570, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITH DNA

Gingival crevicular fluid

MeSH Terms

Conditions

Smoking; Vaping

Interventions

Electronic Nicotine Delivery Systems

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Smoking Devices; Manufactured Materials; Technology, Industry, and Agriculture

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate prof.

Study Record Dates

• First Submitted: January 3, 2026
• First Posted: January 13, 2026
• Study Start: April 30, 2025
• Primary Completion: August 30, 2025
• Study Completion: October 1, 2025
• Last Updated: January 13, 2026

Record last verified: 2025-04

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)