Investigation of the Relationship Between Periodontitis and Alzheimer's Disease in Terms of Blood Parameters

NCT07069244 | Completed

• 1 other identifier: KKARADURAN
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 3

Brief Summary

The aim of this study was to analyse the possible relationship between periodontal inflammation and Alzheimer's disease and to investigate the role of blood parameters in this relationship.

Conditions

Periodontal Disease; Periodontitis; Periodontal Inflammation; Biomarkers / Blood; Blood Cell Count; Alzheimer Disease

Keywords

Alzheimer disease; biomarkers; blood cell count; complete blood count; periodontal disease; periodontitis

Outcome Measures

Primary Outcomes (3)

• Probing pocket depth

  Measurement of the depth of a sulcus or periodontal pocket determined by measuring distance from a gingival margin to the base of the sulcus or pocket with a calibrated periodontal probe.

  Baseline

• Bleeding on probing (BOP)

  The number obtained by dividing the sum of the positive areas by the sum of the examined areas is multiplied by 100 and the value obtained is expressed as the percentage of bleeding at probing (BOP%).

  Baseline

• Periodontal inflammatory surface area (PISA)

  It is the calculation of the area of ulcerated periodontal pockets showing bleeding on probing based on the periodontal epithelial surface area.

  Baseline

Secondary Outcomes (33)

• Clinical attachment level

  Baseline

• Plaque percentage

  Baseline

• Number of teeth

  Baseline

• White blood cell count

  Between baseline and -6th month

• Neutrophil count

  Between baseline and -6th month

Study Arms (2)

Case group

Periodontitis with Alzheimer's disease

Diagnostic Test: Complete Blood Count Test; Diagnostic Test: Periodontal examinations; Diagnostic Test: Clinical Dementia Rating scale; Diagnostic Test: Standardized Mini-Mental Test

Control group

Periodontitis without Alzheimer's disease

Diagnostic Test: Complete Blood Count Test; Diagnostic Test: Periodontal examinations

Interventions

Complete Blood Count Test DIAGNOSTIC_TEST

Complete blood count test provides data on total white blood cell count, neutrophil count, neutrophil percentage, lymphocyte count, lymphocyte percentage, monocyte count, monocyte percentage, eosinophil count, eosinophil percentage, basophil count, basophil percentage, platelet count, platelet distribution width (PDW), mean platelet volume (MPV), plateletcrit (PCT), erythrocyte count, hemoglobin, hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and red blood cell distribution width (RDW). In addition, neutrophil-lymphocyte ratio (NLR), monocyte-lymphocyte ratio (MLR), platelet-leukocyte ratio (PLR), Pan-Immune-Inflammation Value (PIV), Systemic Immune-Inflammation Index (SII), Hemoglobin-to-Red Cell Distribution Width Ratio (HRR) values are also formulated from these parameters.

Case group; Control group

Periodontal examinations DIAGNOSTIC_TEST

With periodontal evaluation, plaque percentage, probing pocket depth, percentage of bleeding on probing, clinical attachment level, number of teeth, periodontal inflammatory surface area (PISA) parameters are recorded.

Case group; Control group

Clinical Dementia Rating scale DIAGNOSTIC_TEST

Stage I: mild Alzheimer's disease, Stage II: moderate Alzheimer's disease, Stage III: severe Alzheimer's disease

Case group

Standardized Mini-Mental Test DIAGNOSTIC_TEST

Cognitive status is assessed with the Standardized Mini-Mental Test.

Case group

Eligibility Criteria

• Age: 50 Years - 90 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Individuals with periodontitis with Alzheimer's disease (Case group), Individuals with periodontitis without Alzheimer's disease (Control group)

You may qualify if:

• Between March 2022 and July 2025, those who applied to the Periodontics Departments of the Dentistry Faculties of Uskudar University, Bezmialem Vakif University and Bolu Abant Izzet Baysal University,
• Informed consent form was obtained during the examination,
• Over 50 years of age,
• Non-smoker and non-alcoholic,
• No psychiatric disorders affecting cognitive status and no medication affecting cognitive status,
• No dental treatment within the last 1 year,
• Periodontal examination was performed by expert periodontologists who calibrated the inter- and intra-examination,
• Individuals diagnosed with periodontitis as a result of periodontal examination and radiographic examination, and
• For individuals diagnosed with Alzheimer's disease: Recording their stage according to the Clinical Dementia Rating (CDR) and Standardized Mini-Mental Test (SMMT) scores

You may not qualify if:

• Individuals with fewer than 10 teeth,
• Individuals with uncontrolled systemic disease,
• Individuals who regularly use anti-inflammatories or corticosteroids

Sponsors & Collaborators

• Uskudar University: lead

Study Sites (3)

Bolu Abant Izzet Baysal University

Bolu, Turkey (Türkiye)

Location

Bezmialem Vakif University

Istanbul, Turkey (Türkiye)

Location

Uskudar University

Istanbul, Turkey (Türkiye)

Location

Related Publications (5)

• Wang RH, Wen WX, Jiang ZP, Du ZP, Ma ZH, Lu AL, Li HP, Yuan F, Wu SB, Guo JW, Cai YF, Huang Y, Wang LX, Lu HJ. The clinical value of neutrophil-to-lymphocyte ratio (NLR), systemic immune-inflammation index (SII), platelet-to-lymphocyte ratio (PLR) and systemic inflammation response index (SIRI) for predicting the occurrence and severity of pneumonia in patients with intracerebral hemorrhage. Front Immunol. 2023 Feb 13;14:1115031. doi: 10.3389/fimmu.2023.1115031. eCollection 2023.

  PMID: 36860868 BACKGROUND

• An P, Zhou X, Du Y, Zhao J, Song A, Liu H, Ma F, Huang G. Association of Neutrophil-Lymphocyte Ratio with Mild Cognitive Impairment in Elderly Chinese Adults: A Case-control Study. Curr Alzheimer Res. 2019;16(14):1309-1315. doi: 10.2174/1567205017666200103110521.

  PMID: 31902361 BACKGROUND

• Zhou C, Liu Y, Bai J, Luo Y, Song J, Feng P. Mean platelet volume is associated with periodontitis: a cross-sectional study. BMC Oral Health. 2024 Apr 16;24(1):461. doi: 10.1186/s12903-024-04223-8.

  PMID: 38627719 BACKGROUND

• Leira Y, Carballo A, Orlandi M, Aldrey JM, Pias-Peleteiro JM, Moreno F, Vazquez-Vazquez L, Campos F, D'Aiuto F, Castillo J, Sobrino T, Blanco J. Periodontitis and systemic markers of neurodegeneration: A case-control study. J Clin Periodontol. 2020 May;47(5):561-571. doi: 10.1111/jcpe.13267. Epub 2020 Mar 12.

  PMID: 32027386 BACKGROUND

• Tosatti JAG, Pereira JD, Loures CMG, Fraga VG, Magalhaes CA, Eugenio RDAC, Guimaraes HC, Resende EPF, de Souza LC, Carvalho MDG, Caramelli P, Gomes KB. Complete blood count and systemic inflammation indices in individuals with Alzheimer's disease: A case-control study. J Clin Neurosci. 2025 Feb;132:111011. doi: 10.1016/j.jocn.2024.111011. Epub 2024 Dec 28.

  PMID: 39733506 BACKGROUND

MeSH Terms

Conditions

Periodontal Diseases; Periodontitis; Alzheimer Disease

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases; Dementia; Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Tauopathies; Neurodegenerative Diseases; Neurocognitive Disorders; Mental Disorders

Study Officials

• OZLEM GELISIN

  Dr. Sadi Konuk Training and Research Hospital

  STUDY CHAIR

• AHMET AYDOGDU

  Istanbul Galata University

  STUDY CHAIR

• SADIYE GUNPINAR

  Bezmialem Vakif University

  STUDY CHAIR

• ZEYNEP AKGUL

  Abant Izzet Baysal University

  STUDY CHAIR

• KUBRA KARADURAN

  Uskudar University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant professor

Study Record Dates

• First Submitted: July 5, 2025
• First Posted: July 16, 2025
• Study Start: August 5, 2025
• Primary Completion: October 3, 2025
• Study Completion: November 3, 2025
• Last Updated: November 17, 2025

Record last verified: 2025-11

Locations

TU (3)