NCT07036809

Brief Summary

The goal of this observational study is to evaluate the levels of Antithrombin III, Protein C, Protein S, and D-dimer in patients with Acute Leukemia (Acute Lymphoblastic Leukemia (ALL) and Acute Myeloid Leukemia (AML)). The main questions it aims to answer are: What are the levels of Antithrombin III, Protein C, Protein S, and D-dimer in patients with Acute Lymphoblastic Leukemia? What are the levels of Antithrombin III, Protein C, Protein S, and D-dimer in patients with Acute Myeloid Leukemia? Researchers will compare patients with Acute Leukemia (30 with ALL and 30 with AML) to 30 healthy control subjects to see if there are significant differences in the levels of Antithrombin III, Protein C, Protein S, and D-dimer. Participants will: Undergo full history and clinical examination. Have routine hematological analysis including Complete Blood Count (CBC), blood film, Erythrocyte sedimentation rate (ESR), Prothrombin Time Test and INR (PT/INR), and partial thromboplastin time (PTT). Have specific tests for quantitative measurement of Antithrombin III, Protein C, Protein S, and D-dimer. Undergo Flow cytometry immunophenotyping.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
90

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Jun 2023

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 1, 2023

Completed
2 years until next milestone

First Submitted

Initial submission to the registry

June 17, 2025

Completed
8 days until next milestone

First Posted

Study publicly available on registry

June 25, 2025

Completed
3 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

October 1, 2025

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

October 1, 2025

Completed
Last Updated

January 29, 2026

Status Verified

January 1, 2026

Enrollment Period

2.3 years

First QC Date

June 17, 2025

Last Update Submit

January 27, 2026

Conditions

Keywords

acute leukemiaAntithrombin IIIprotein CProtein SD Dimer

Outcome Measures

Primary Outcomes (1)

  • Assessment of Antithrombin III, Protein C, Protein S, and D-dimer Levels in Acute Leukemia Patients

    To evaluate and compare the quantitative levels of Antithrombin III, Protein C, Protein S, and D-dimer in patients diagnosed with Acute Lymphoblastic Leukemia (ALL) and Acute Myeloid Leukemia (AML) against healthy control subjects. This assessment will help determine any significant differences in these coagulation parameters within the different acute leukemia subtypes and in comparison to healthy individuals.

    at the time of patient recruitment for the study.

Study Arms (3)

acute lymphoblastic leukemia

patients diagnosed with acute lymphoblastic leukemia.

Diagnostic Test: Antithrombin IIIDiagnostic Test: Protein CDiagnostic Test: Protein SDiagnostic Test: D-dimerDiagnostic Test: Flow cytometry immunophenotyping

acute myeloid leukemia

patients diagnosed with acute myeloid leukemia.

Diagnostic Test: Antithrombin IIIDiagnostic Test: Protein CDiagnostic Test: Protein SDiagnostic Test: D-dimerDiagnostic Test: Flow cytometry immunophenotyping

control group

controlled group include healthy persons not suffering from acute leukemia either clinically or laboratory

Diagnostic Test: Antithrombin IIIDiagnostic Test: Protein CDiagnostic Test: Protein SDiagnostic Test: D-dimerDiagnostic Test: Flow cytometry immunophenotyping

Interventions

D-dimerDIAGNOSTIC_TEST

quantitative measurement( ug/ml)

acute lymphoblastic leukemiaacute myeloid leukemiacontrol group

Flow cytometry immunophenotyping

acute lymphoblastic leukemiaacute myeloid leukemiacontrol group
Antithrombin IIIDIAGNOSTIC_TEST

quantitative measurement(%)

acute lymphoblastic leukemiaacute myeloid leukemiacontrol group
Protein CDIAGNOSTIC_TEST

quantitative measurement(%)

acute lymphoblastic leukemiaacute myeloid leukemiacontrol group
Protein SDIAGNOSTIC_TEST

quantitative measurement(%)

acute lymphoblastic leukemiaacute myeloid leukemiacontrol group

Eligibility Criteria

Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

This study will include 90 subjects, without regard to their sex or age. The subjects will be classified into two main groups: a patient group and a control group. Patient Group (Group 1): This group will consist of 60 patients suffering from acute leukemia. It is further divided into two subgroups: Group 1A: 30 patients diagnosed with acute lymphoblastic leukemia (ALL). Group 1B: 30 patients diagnosed with acute myeloid leukemia (AML). Patients will be selected from the outpatient clinic, internal medicine, and oncology departments at both Al Hussein and Sayed Galal University Hospitals, provided they give appropriate consent to participate. Control Group (Group 2): This group will consist of 30 healthy individuals who are not suffering from acute leukemia, as determined both clinically and through laboratory tests.

You may qualify if:

  • patients with leukemia in group 1 and 2.
  • willing to participate in the study.

You may not qualify if:

  • refusing to participate.
  • patients with know to have either hereditary thrombophilia or current thrombosis.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Al-Hussein University Hospital

Cairo, 11633, Egypt

Location

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
RETROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
lecturer and consultant at Obstetrics and Gynecology Department.

Study Record Dates

First Submitted

June 17, 2025

First Posted

June 25, 2025

Study Start

June 1, 2023

Primary Completion

October 1, 2025

Study Completion

October 1, 2025

Last Updated

January 29, 2026

Record last verified: 2026-01

Data Sharing

IPD Sharing
Will share

Individual Participant Data (IPD) from this study, including de-identified demographic information, clinical examination findings, routine hematological analysis results (Complete Blood Count, Erythrocyte Sedimentation Rate, Prothrombin Time/INR, partial thromboplastin time), and specific test results (Antithrombin III, Protein C, Protein S, D-dimer, and Flow cytometry immunophenotyping), will be made available. The data will be provided in a secure, anonymized format suitable for secondary analysis (e.g., CSV or statistical software file types). This sharing aims to foster further research, facilitate meta-analyses, and enable validation studies by the wider scientific community, while strictly safeguarding participant privacy.

Shared Documents
STUDY PROTOCOL, SAP, ICF
Time Frame
The de-identified IPD will become available for access upon publication of the study's main results. The dataset will remain accessible for a period of ten (10) years following the initial publication date.
Access Criteria
Access to the IPD will be granted to qualified researchers for legitimate scientific purposes, subject to the following criteria: Submission of a detailed research proposal outlining the scientific objectives, methodology for analysis, and a commitment to ethical research practices. Approval of the research proposal by a designated Data Access Committee or institutional review board, if applicable, to ensure scientific merit and ethical considerations. Execution of a formal Data Use Agreement (DUA) with the study principal investigators, specifying the terms of data usage, confidentiality, data security, and a strict prohibition against any attempt at re-identification of participants. Agreement to acknowledge the original study and its investigators in any resulting publications or presentations. Confirmation that the proposed research aligns with the informed consent provided by study participants.

Locations