Pre-Sleep Creatine Enhances Anaerobic Power in Recreationally Active Females

NCT06937190 | Recruiting

• 1 other identifier: 162593019
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

This study is a randomized, double-blind, placebo-controlled trial designed to examine the effects of pre-sleep creatine monohydrate supplementation on anaerobic performance and muscle damage markers in recreationally active females. Participants will be randomly assigned to receive either 5 grams of creatine monohydrate or a placebo 30 minutes before sleep for seven consecutive days. Anaerobic performance will be assessed using the Wingate Anaerobic Test, evaluating peak and average power output and fatigue index. Blood samples will be collected before and after the supplementation period to analyze markers of muscle damage, including creatine kinase and lactate dehydrogenase. This study aims to provide insight into the efficacy of short-term, time-specific creatine supplementation for enhancing performance and recovery.

Conditions

Anaerobic Performance; Sports Nutrition; Exercise Recovery; Muscle Fatigue; Creatine Supplementation; Recreational Physical Activity; Female Athletes

Keywords

Creatine Monohydrate; Pre-Sleep Supplementation; Anaerobic Power; Wingate Test; Muscle Damage Markers; Creatine Kinase (CK); Lactate Dehydrogenase (LDH); Female Exercise Performance; Nutritional Ergogenic Aid; Resistance Training; Recovery Enhancement

Outcome Measures

Primary Outcomes (1)

• Average Power Output (Watts) - 30-Second Wingate Test

  Average anaerobic power output will be calculated across the 30-second Wingate cycling test.

  Baseline and post-intervention (Day 0 and Day 7)

Secondary Outcomes (4)

• Peak Power Output (Watts) - Wingate Test

  Baseline and post-intervention (Day 0 and Day 7)

• Fatigue Index (%) - Wingate Test

  Baseline and post-intervention (Day 0 and Day 7)

• Serum Creatine Kinase (CK) Activity

  Baseline and 24 hours post Wingate Test (Day 0 and Day 8)

• Serum Lactate Dehydrogenase (LDH) Activity

  Baseline and 24 hours post Wingate Test (Day 0 and Day 8)

Study Arms (2)

Creatine Monohydrate Supplementation

EXPERIMENTAL

Participants will receive 5 grams of creatine monohydrate powder dissolved in water, flavored with a sugar-free agent, 30 minutes before sleep each night for 7 days.

Dietary Supplement: Creatine Monohydrate

Placebo Supplementation

PLACEBO COMPARATOR

Participants will receive 5 grams of a placebo powder (maltodextrin), visually identical and similarly flavored to the creatine supplement, 30 minutes before sleep each night for 7 days.

Dietary Supplement: Placebo (Maltodextrin)

Interventions

Creatine Monohydrate DIETARY_SUPPLEMENT

Participants will receive 5 grams of creatine monohydrate powder dissolved in water, flavored with a sugar-free agent, 30 minutes before sleep each night for 7 days.

Creatine Monohydrate Supplementation

Placebo (Maltodextrin) DIETARY_SUPPLEMENT

Visually identical placebo (maltodextrin powder, 5 g/day), flavored with a sugar-free agent, dissolved in water and consumed 30 minutes before sleep for 7 consecutive days.

Placebo Supplementation

Eligibility Criteria

• Age: 18 Years - 25 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Female, aged 18 to 25 years
• Recreationally active (engaging in structured resistance training 3-4 times per week for the past 6 months)
• Regular menstrual cycles (used for scheduling purposes)
• Able and willing to provide informed consent
• Agreement to maintain normal diet and training routines throughout the study

You may not qualify if:

• History of musculoskeletal injury affecting lower or upper limbs within the past 6 months
• Known allergy or intolerance to creatine or maltodextrin
• Use of performance-enhancing substances or supplements within the last 30 days
• Use of recovery modalities such as massage, cryotherapy, or compression garments during the study period
• Current use of anti-inflammatory medication
• Pregnancy or breastfeeding
• Inability to comply with study protocol or scheduled assessments

Sponsors & Collaborators

• Ankara Yildirim Beyazıt University: lead

Study Sites (1)

Islamic Azad University, Shabestar Branch

Shabestar, East Azerbaijan Province, 5381637183, Iran

RECRUITING

MeSH Terms

Interventions

Creatine; maltodextrin

Intervention Hierarchy (Ancestors)

Guanidines; Amidines; Organic Chemicals; Amino Acids; Amino Acids, Peptides, and Proteins

Central Study Contacts

Morteza Jourkesh, PhD

CONTACT

+98 938 278 4093; mjourkesh@iau.ac.ir

Caglar Soylu, phD

CONTACT

+90 5078593595; fztcaglar5187@gmail.com

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Masking Details: Neither the participants nor the investigators know which intervention the participant is receiving.
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Asisstant Professor

Model Details: Participants are randomly assigned to one of two groups: a creatine supplementation group or a placebo group, and they remain in that group for the duration of the trial.

Study Record Dates

• First Submitted: April 15, 2025
• First Posted: April 22, 2025
• Study Start: April 1, 2025
• Primary Completion: May 10, 2025
• Study Completion: July 10, 2025
• Last Updated: April 22, 2025

Record last verified: 2025-04

Data Sharing

• IPD Sharing: Will not share

Locations

IR (1)