Improving the Anabolic Action of Plant-based Foods Using Complementary Proteins

NCT06781723 | Completed

• 2 other identifiers: IRB24-05827007302
• Study Type: interventional
• Target: 11
• Locations: 1 country
• Sites: 1

Brief Summary

In a crossover design 10 young healthy adults (20-35 y) will receive stable isotope tracer infusions and perform a single bout of resistance exercise. Immediately after exercise participants will ingest either 246 g of rice and beans or its constituent macronutrients as isolated amino acids, carbohydrates, fiber, and fat. Repeated blood and muscle biopsies will be collected to determine amino acid concentrations, anabolic signaling and myofibrillar protein synthesis rates during the trials.

Conditions

Health; Recreational Activities; Muscle Protein Synthetic Response to Protein; Muscle Protein Synthesis

Keywords

protein; metabolism; plant protein; vegan; protein synthesis; complementary proteins

Outcome Measures

Primary Outcomes (2)

• Myofibrillar protein fractional synthesis rate

  Myofibrillar protein synthesis rates will be assessed during the postabsorptive and postprandial periods of experimental primed-constant stable isotope infusions.

  Basal FSR (from -150 to - 30 min), Postprandial FSR (from -30 to 300min)

• Plasma Amino Acid Concentrations

  Amino acid concentrations will be assessed in the postabsorptive state (-210, -150, -90, -30, 0 min) and postprandial state (30, 60, 90, 120, 180, 240, 300 min) via LC/MS/MS.

  Post absorptive (-210, -150, -90, -30, 0 min); Post prandial (30, 60, 90, 120, 180, 240, 300 min)

Secondary Outcomes (1)

• Activation of Anabolic Signaling Targets

  -150 min, -30 min, 300 min

Study Arms (2)

Rice and Beans (COMP)

EXPERIMENTAL

Rice and Beans After resistance exercise, participants will ingest a meal of 123g Rice, 123g Black Beans (246 g; 575kcal, 20 g Protein, 121 g Carbohydrate, 0.7 g Fat, 1.78 g Fat)

Behavioral: Resistance Exercise; Other: Rice and Beans

Isolated Nutrients (ISO)

ACTIVE COMPARATOR

This condition is isonitrogenous and a similar nutrient profile to the experimental complementary mixed meal. The drink is comprised of crystalline amino acids, carbohydrate, fat, and fiber that is matched to rice and beans meal composition.

Behavioral: Resistance Exercise; Dietary Supplement: Isolated Mixture

Interventions

Resistance Exercise BEHAVIORAL

Participants will perform leg press and leg extension immediately prior to ingestion of rice and beans or isolated mixture.

Isolated Nutrients (ISO); Rice and Beans (COMP)

Isolated Mixture DIETARY_SUPPLEMENT

Participants will ingest isolated mixture of crystalized amino acids, maltodextrin, fiber and oil immediately after resistance exercise.

Also known as: ISO

Isolated Nutrients (ISO)

Rice and Beans OTHER

Participants will ingest cooked rice and beans meal immediately after resistance exercise.

Rice and Beans (COMP)

Eligibility Criteria

• Age: 20 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age 20-35 yrs
• Pre-menopausal
• Recreationally-active
• Weight stable for prior 6 months

You may not qualify if:

• Age outside of range (20 - 35 yrs)
• Pregnancy
• Irregular menstrual cycles (i.e., \~21 day or \~35 day cycle)
• Participation in previous research using \[13C6\]phenylalanine
• Participation in other ongoing research that interferes with this study (e.g., conflicting diet, activity interventions, etc.)
• Any hospitalization or surgery for a metabolic, cardiovascular, or neuromusculoskeletal complication within the past year
• Allergy or hypersensitivity to local anesthetics, latex, or adhesives (bandages, medical tape, etc.)
• Excess scarring after injury
• History of excess bleeding after cut
• Chronic or frequent dizziness/fainting, and arm or leg weakness/numbness
• Arthritis
• Tumors
• Mental Illness
• Hepatorenal, cardiovascular musculoskeletal, autoimmune, or neurological disease or disorder
• Predisposition to hypertrophic scarring or keloid formation

Sponsors & Collaborators

• University of Illinois at Urbana-Champaign: lead

Study Sites (1)

Louise Freer Hall

Urbana, Illinois, 61801, United States

Location

MeSH Terms

Interventions

Resistance Training

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Model Details: Randomized crossover design

Study Record Dates

• First Submitted: October 7, 2024
• First Posted: January 17, 2025
• Study Start: September 5, 2024
• Primary Completion: April 1, 2025
• Study Completion: April 1, 2025
• Last Updated: May 8, 2025

Record last verified: 2025-05

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)