Time Course of Postprandial Protein Metabolism

NCT04121689 | Completed

• 1 other identifier: 14234
• Study Type: interventional
• Target: 7
• Locations: 1 country
• Sites: 1

Brief Summary

The anabolic action of 'fast' whey protein on the regulation of postprandial muscle protein synthesis has been established to be short-lived in healthy young adults. Our aim was assess the time course of anabolic signaling events and stimulation of muscle protein synthesis rates (MPS) after ingestion of a food source that represents a more typical meal-induced pattern of aminoacidemia, namely milk protein concentrate, in healthy young males.

Conditions

Muscle Protein Synthesis; Protein Metabolism

Keywords

Protein; Amino Acids; Anabolic Signaling; Muscle Mass

Outcome Measures

Primary Outcomes (1)

• Fractional synthesis rates of myofibrillar protein

  Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion. This will allow us assess the timecourse of muscle protein synthesis after eating.

  8 hours

Secondary Outcomes (2)

• Phosphorylation of muscle anabolic signaling

  8 hours

• Exogenous rate of phenylalanine appearance

  5 hours

Study Arms (1)

Milk Protein Concentrate

EXPERIMENTAL

Seven young men (age: 22±1 y) will undergo repeated blood and biopsy sampling during primed continuous L-\[ring-2H5\]phenylalanine and L-\[1-13C\]leucine tracer infusions, and ingested 38 g of L-\[1-13C\]phenylalanine- and L-\[1-13C\]leucine-labeled milk protein concentrate

Dietary Supplement: Milk Protein Concentrate

Interventions

Milk Protein Concentrate DIETARY_SUPPLEMENT

The macronutrient composition and energy of the milk protein beverage provided to participants is 38 g protein (3.46 g leucine), 4.17 g carbohydrate, and 1.4 g fat. The milk protein met all chemical and bacteriologic specifications for human consumption. The L-\[1-13C\]phenylalanine and L-\[1-13C\]leucine enrichments in the milk protein concentrate averaged 38.3 and 10.8 mole percent excess (MPE), respectively.

Milk Protein Concentrate

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Males
• Aged between 18-35 years
• Healthy, recreationally active
• BMI \< 30 kg/m2

You may not qualify if:

• Smoking
• Allergies to milk proteins
• Vegetarians
• Diagnosed GI tract diseases
• Female
• Arthritic conditions
• A history of neuromuscular problems
• Previous participation in amino acid tracer studies
• Predisposition to hypertrophic scarring or keloid formation
• Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).

Sponsors & Collaborators

• University of Illinois at Urbana-Champaign: lead

Study Sites (1)

Freer Hall

Urbana, Illinois, 61801, United States

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: September 3, 2019
• First Posted: October 10, 2019
• Study Start: October 1, 2014
• Primary Completion: November 1, 2014
• Study Completion: August 26, 2019
• Last Updated: October 10, 2019

Record last verified: 2019-08

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)