Oral 'Breath Test' to Measure Anabolic Sensitivity to a Protein Meal Across the Age and Physical Activity Spectrum

NCT06744491 | Not Yet Recruiting

• 1 other identifier: 00044004
• Study Type: observational
• Target: 80
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to determine how anabolic sensitivity, in response to the ingestion of a liquid protein meal, differs across the lifespan, between biological sexes, and with varying physical activity levels.

Conditions

Inactivity, Physical; Aging; Healthy Aging; Growth & Development; Sex Differences; Anabolic Sensitivity; Protein Metabolism; Physical Activity

Keywords

anabolic sensitivity; aging; growth and development; physical activity; skeletal muscle; stable isotopes

Outcome Measures

Primary Outcomes (1)

• Amino acid oxidation and net protein balance

  Amino acid oxidation and net protein balance assessed by oral 1-13C Leucine tracer. Net protein balance is derived from the difference between amino acid intake (known) and total amino acid oxidation over the 6h post-prandial measurement period.

  6 Hours

Study Arms (4)

Children

Children as defined by maturity offset via age from peak height velocity (aPHV \< -1.0 years).

Adolescent

Adolescents as defined by maturity offset via age from peak height velocity (aPHV -0.5 to +1.5 years)

Young Adults

Young adults as defined by chronological age between ages 18 - 35 years

Older Adults

Older adults as defined by chronological age between ages 60 - 80 years

Eligibility Criteria

• Age: 8 Years - 80 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Convenience sample of individuals who qualify for the corresponding cohorts based on inclusion/exclusion criteria stated.

You may qualify if:

• Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone)
• Chronological age for Healthy Young (age: 18-35 years) or Older (age: 60-80 years) adults
• Chronological age between 8 to 16 years, with maturity offset \< -1 year from age of Peak Height Velocity (aPHV) for children and between -0.5 to +1.5 years from aPHV in adolescent participants
• BMI between normal to overweight (18.5 - 29.9 kg/m2)

You may not qualify if:

• Regular use of nonsteroidal anti-inflammatory dugs (with the exception of daily low-dose aspirin)
• use of anticoagulants
• use of a walker, cane, or assistive walking device
• infectious or gastrointestinal disease
• inability to comply with study protocol (e.g., unable to track diet)
• regular tobacco use
• self-reported illicit drug use (e.g., growth hormone, testosterone, etc.)
• diagnosed chronic illness (e.g., type 2 diabetes, heart disease, thyroid disease)
• pregnant
• hormonal replacement therapy

Sponsors & Collaborators

• University of Toronto: lead

Study Sites (1)

Goldring Center for High Performance Sport

Toronto, Ontario, M5S 2C9, Canada

Location

Biospecimen

Retention: SAMPLES WITH DNA

Urine and saliva samples will be retained, though not for the purpose of DNA extraction.

MeSH Terms

Conditions

Sedentary Behavior; Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Central Study Contacts

Daniel R Moore, PhD

CONTACT

4169464088; dr.moore@utoronto.ca

Hugo JW Fung, PhD Candidate

CONTACT

hugojernwai.fung@utoronto.ca

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor, Muscle Physiology

Study Record Dates

• First Submitted: December 16, 2024
• First Posted: December 20, 2024
• Study Start: December 20, 2024
• Primary Completion: May 30, 2025
• Study Completion: August 31, 2025
• Last Updated: December 20, 2024

Record last verified: 2024-11

Locations

CA (1)