The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains.

NCT06403241 | Completed

• 1 other identifier: Rowerscometetive2023
• Study Type: observational
• Target: 13
• Locations: 1 country
• Sites: 1

Brief Summary

The study aimed to check the influence of timing and composition of diet on markers of gut injury, endotoxemia and stress.

Conditions

Endothelial Dysfunction

Keywords

athlete's diet; endotoxemia; cortisol; gut injury; gut permeability; exercise

Outcome Measures

Primary Outcomes (5)

• I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury

  I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period

• Claudin to measure tight junction leakage

  Zonulin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period

• LBP (lipopolysaccharide binding protein) to measure endotoxin

  LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period

• Lactic acid to measure fatigue after the race

  Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test

• Corisol

  Stress hormoneconcentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period

Secondary Outcomes (15)

• energy

  whole day (24hours) before test, in the morning before the test

• protein

  whole day (24hours) before test, in the morning before the test

• fat

  whole day (24hours) before test, in the morning before the test

• carbohydrate

  whole day (24hours) before test, in the morning before the test

• glucose

  whole day (24hours) before test, in the morning before the test

Interventions

2000 meter ergometer test OTHER

Rowers performed 2000m ergometer test.A 2,000-m time trial is a standard test used to assess performance in rowers.

Eligibility Criteria

• Age: 18 Years - 23 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Male rowers age 18 - 22 years.

You may qualify if:

• Membership in Polish Rowing Team,
• minimum five years of training,
• total training time minimum of 240 minutes,
• filling out a food diary,
• finishing 2000-meter ergometer test.

You may not qualify if:

• Probiotics within the last three months,
• prebiotics within the last three months
• antibiotic therapy within the last three months,
• dietary regime,
• gastrointestinal diseases,
• lactose intolerance

Sponsors & Collaborators

• Poznan University of Physical Education: lead

Study Sites (1)

Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow

Gorzów Wielkopolski, Lubusz Voivodeship, 66-400, Poland

Location

MeSH Terms

Conditions

Endotoxemia; Motor Activity

Condition Hierarchy (Ancestors)

Bacteremia; Sepsis; Infections; Toxemia; Systemic Inflammatory Response Syndrome; Inflammation; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Behavior

Study Officials

• Anna Skarpańska-Stejnborn, professor

  Poznan University of Physical Education

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: April 26, 2024
• First Posted: May 7, 2024
• Study Start: May 1, 2023
• Primary Completion: June 1, 2023
• Study Completion: June 30, 2023
• Last Updated: May 7, 2024

Record last verified: 2023-06

Locations

PL (1)