Effect of 2000-meter Rowing Test on Parameters of Intestinal Integrity in Elite Rowers During Competitive Phase

NCT06372262 | Completed

• 1 other identifier: Rowers2022
• Study Type: observational
• Target: 18
• Locations: 1 country
• Sites: 1

Brief Summary

The study aimed to check the 2000m ergometer test on markers of gut permeability in the competitive phase of rowers. 18 members of the Polish rowing team took part in the study.

Conditions

Endothelial Dysfunction

Keywords

endotoxin; gastrointestinal symptoms; gut permeability

Outcome Measures

Primary Outcomes (5)

• I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury

  I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

• zonulin to measure tight junction leakage

  zonulin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

• LBP (lipopolysaccharide binding protein) to measure endotoxin

  LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

• LPS (lipopolysaccharide) to measure endotoxin

  LPS concentration measured in blood \[EU/Ll\] using commercially available enzyme-linked immunosorbent assays

  Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period

• Lactic acid to measure fatigue after the race

  Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)

  Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.

Secondary Outcomes (8)

• energy

  day before test

• fiber intake

  day before test

• protein

  day before test

• carbohydrate

  day before test

• body mass

  in the morning before the test

Interventions

ergometer test OTHER

2000meter rowing ergometer test

Eligibility Criteria

• Age: 18 Years - 23 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Male rowers age 18 - 22 years.

You may qualify if:

• minimum five years of training,
• five training sessions per week minimum,
• total training time minimum of 240 minutes,
• membership in the Polish Rowing Team,
• finishing the 2000-meter ergometer test.

You may not qualify if:

• antibiotic therapy within the last three months,
• probiotics and prebiotics within the last three months,
• dietary regime,
• gastrointestinal diseases.

Sponsors & Collaborators

• Poznan University of Physical Education: lead

Study Sites (1)

Poznan University of Physical Education

Poznan, Poland

Location

Study Officials

• Anna Skarpańska-Stejnborn, proffessor

  Poznan University of Physical Education

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 19, 2024
• First Posted: April 17, 2024
• Study Start: June 1, 2022
• Primary Completion: June 30, 2022
• Study Completion: June 30, 2022
• Last Updated: April 17, 2024

Record last verified: 2022-06

Data Sharing

• IPD Sharing: Will not share

Locations

PL (1)