Myokine Identification Following Acute Exercise

NCT06223035 | Completed

• 1 other identifier: LOL-1044
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

Exercise stimulates a cascade of responses within the human body. For example, exercise results in the release of proteins into the circulation which communicate with cells and organs throughout the body. In fact, recent human research identified more than 600 proteins are released into the blood circulation following short-term exercise, many of which are predicted to come from the skeletal muscle and target the fat tissue. However, identification of these muscle-secreted proteins and their target tissue (i.e. fat tissue) remains extremely challenging. This challenge is because tissue needs to be collected from multiple sites (skeletal muscle and fat) and at multiple timepoints (before and after exercise). This study seeks to address these challenges through the collection of fat and blood both before and after short-term exercise followed by protein detection (of the blood) and gene expression (of the fat tissue).

Conditions

Adiposity

Outcome Measures

Primary Outcomes (1)

• Identification and quantification of exercise-induced proteins released into the blood through the use of proteomic analysis.

  The change in protein abundance within the circulation will be identified by using proteomic-based detection methods. Proteins detected from the control timepoint (before exercise), will be compared to the three timepoints following exercise.

  Before exercise, immediately following exercise, 1-hour following exercise, 3-hours following exercise

Secondary Outcomes (1)

• Characterize changes in gene expression of the subcutaneous adipose tissue following exercise through the use of bulk RNA-sequencing.

  1 week pre-intervention, 1-hour following, 3-hours following

Study Arms (1)

Secretome characterization and signaling to the adipose tissue

EXPERIMENTAL

To identify possible exercise stimulated secreted proteins which signal to the adipose tissue, subcutaneous adipose and blood will be collected both before and at multiple timepoints following a VO2max test. Adipose will be collected from the abdominal subcutaneous region and blood will be collected from the arm. Blood will be analyzed for change in protein abundance and adipose will be analyzed for change in gene expression.

Other: Exercise

Interventions

Exercise OTHER

Participants will take part in a VO2max test. This will include a graded exercise test (GXT) on a motorized treadmill while wearing a one-way, non-rebreathing valve and nose clip that allows for the evaluation of all expired air. Specifically, we will incorporate the Ellestad Protocol. The Ellestad Protocol is divided into 8 phases which gradually increases in speed and incline throughout the test. The VO2max test will continue until the participant reaches volitional fatigue defined by reaching a rate of perceived exertion (RPE) greater than 19 (using the Borg RPE Scale), OR a respiratory exchange ratio (RER) reaching above 1.1, OR a participant choosing to cease exercise for any reason.

Secretome characterization and signaling to the adipose tissue

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• passing the PARQ questionnaire (i.e. answering "NO" to all questions)
• being between the ages of 18-45
• being moderately active (defined as participating in 30 minutes of recreational activity at least once per week for the past 3 months but no more than 3 times per week)
• non-smoking
• free of metabolic or cardiovascular diseases

You may not qualify if:

• unhealed fractures
• thrombophlebitis (blood clots)
• recent surgery of the upper or lower extremities (any surgeries in past 6 months at the ankle or foot, or in the past year at the knee, hip, shoulder or elbow)
• recent uncontrolled bruising
• recent muscle, bone or joint damage which interferes with regular physical activity
• taking part in vigorous physical activity more than 3 times per week
• any prior sensations of chest pain during exercise.
• individuals who are pregnant as determined by an on-site pregnancy test
• individuals who are currently taking exogenous hormones
• individuals who chronically consume NSAIDS or other anti-inflammatory medication (every day for the previous 3 months)
• failing the PARQ questionnaire as indicated by answering "YES" to any of the questions
• Having a BMI \>32
• Any medical condition that in the opinion of the investigator will confound interpretation of data for analysis.

Sponsors & Collaborators

• Rockefeller University: lead
• Hospital for Special Surgery, New York: collaborator

Study Sites (1)

Rockefeller University

New York, New York, 10065, United States

Location

Related Publications (1)

• Walker JM, Garcet S, Aleman JO, Mason CE, Danko D, Butler D, Zuffa S, Swann JR, Krueger J, Breslow JL, Holt PR. Obesity and ethnicity alter gene expression in skin. Sci Rep. 2020 Aug 21;10(1):14079. doi: 10.1038/s41598-020-70244-2.

  PMID: 32826922 BACKGROUND

MeSH Terms

Conditions

Obesity

Interventions

Exercise

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Luke Olsen, PhD

  The Rockefeller University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 30, 2023
• First Posted: January 25, 2024
• Study Start: April 18, 2024
• Primary Completion: August 9, 2024
• Study Completion: August 9, 2024
• Last Updated: March 10, 2025

Record last verified: 2025-03

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)