Effect of Passive Heat Therapy on Metabolism

NCT06147557 | Completed

• 1 other identifier: LithuananianSportsU-16
• Study Type: interventional
• Target: 25
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this prospective interventional study is to examine if repeated brief hot stimuli affects glucose metabolism and substrate oxidation in young non-obese adults. Young adult participants were asked to participate in fourteen 5-min procedures involving whole body passive heating at 45°C water. The main question it aims to answer is: "Does repeated brief noxious heat stimuli is sufficient to improve glucose tolerance, insulin sensitivity, and fat oxidation in young non-obese adults?"

Conditions

Healthy

Outcome Measures

Primary Outcomes (9)

• Changes in catecholamines concentration (ng/mL)

  The venous plasma adrenaline and noradrenaline concentrations (in ng/mL) were measured using enzyme-linked immunosorbent assay kits and a Spark multimode microplate reader

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Changes in cytokines concentration (pg/mL)

  The venous serum interleukin-6 and tumor necrosis factor alpha concentrations (in pg/mL) were measured using enzyme-linked immunosorbent assay kits and a Spark multimode microplate reader

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in glucose concentration (mmol/L)

  The venous glucose concentration (in mmol/L) was measured using a Glucocard X-mini plus meter.

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in insulin concentration (μIU/mL)

  The venous serum insulin concentrations (in μIU/mL) were measured using enzyme-linked immunosorbent assay kits (Cat. No. E-EL-H2237, Elabscience, China) and a Spark multimode microplate reader (Tecan, Austria).

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in insulin sensitivity

  Indices for insulin sensitivity/resistance assessment were computed using the homeostatic model assessment for insulin resistance, quantitative insulin-sensitivity check index (QUICKI), and the Matsuda insulin sensitivity index were calculated.

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in substrate oxidation

  Oxygen consumption (VO2) and carbon dioxide (VCO2) output on a breath-by breath basis using a stationary MetaLyzer® 3B spiroergometry system (Cortex Biophysik GmbH) was measured at rest, and the respiratory quotient (RQ=VCO2/VO2) was computed to determine substrate utilisation.

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in fat oxidation (g/min

  Oxygen consumption (VO2) and carbon dioxide (VCO2) output on a breath-by breath basis using a stationary MetaLyzer® 3B spiroergometry system (Cortex Biophysik GmbH) was measured at rest, and the fat oxidation (FATox; g/min) was calculated by using the equation: FATox = 1.67 × VO2 - 1.67 × VCO2,

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in fat oxidation (g/min)

  Oxygen consumption (VO2) and carbon dioxide (VCO2) output on a breath-by breath basis using a stationary MetaLyzer® 3B spiroergometry system (Cortex Biophysik GmbH) was measured at rest, and the carbohydrate oxidation (CARBox; g/min) was calculated by using the equation: CARBox = 4.55 × VCO2 - 3.21 × VO2

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in resting energy expenditure (kcal/day)

  Oxygen consumption (VO2) and carbon dioxide (VCO2) output on a breath-by breath basis using a stationary MetaLyzer® 3B spiroergometry system (Cortex Biophysik GmbH) was measured at rest, and the resting energy expenditure (REE; kcal/day) was calculated by using the Weir equation: REE = (3.941(VO2) + 1.106(VCO2)) × 1440.

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

Secondary Outcomes (7)

• Change in body mass (kg)

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in fat mass (kg)

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in fat mass (%)

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in fat free mass (kg)

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

• Change in fat free mass (%)

  Pre-condition, post-condition (after 14 days), and after 1 month recovery

Study Arms (1)

Heat therapy

EXPERIMENTAL

14 heat sessions of whole-body immersed in a 45°C water bath for 5 minutes.

Procedure: Heat therapy

Interventions

Heat therapy PROCEDURE

14 heat sessions, during each session subjects were fully immersed in a 45°C water bath for 5 minutes.

Heat therapy

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy non-obese (BMI between 18.5 and 29.9 kg/m2) males and females;
• no diseases, or conditions that could be worsened by exposure to acute hot water and affect experimental variables;
• no participation in any excessive formal physical exercise or sports program, temperature manipulation program or exposure to extreme temperatures.

You may not qualify if:

• smokers;
• obesity (BMI greater than 30 kg/m2);
• needle phobia;
• taking medication and/or dietary supplements that may affect experimental variables.

Sponsors & Collaborators

• Lithuanian Sports University: lead

Study Sites (1)

Institute of Sport Science and Innovations

Kaunas, Lithuania

Location

MeSH Terms

Interventions

Diathermy

Intervention Hierarchy (Ancestors)

Hyperthermia, Induced; Therapeutics

Study Officials

• Marius Brazaitis, PhD

  Lithuanian Sports University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 15, 2023
• First Posted: November 27, 2023
• Study Start: October 1, 2020
• Primary Completion: May 31, 2022
• Study Completion: May 31, 2022
• Last Updated: November 27, 2023

Record last verified: 2023-11

Data Sharing

• IPD Sharing: Will not share

Locations

LI (1)