RibOSE - Glucose and Resistance Exercise Training

NCT04545190 | Completed DMC

• 1 other identifier: Trainome#024_2020
• Study Type: interventional
• Target: 16
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of the study is to investigate the effects of ingesting glucose during five bouts of resistance exercise on muscle biological charateristics in m. vastus lateralis of moderately trained healthy individuals (20-45 years of age, n=20)

Conditions

Healthy

Keywords

Strength/resistance training; Glucose supplementation; Skeletal muscle

Outcome Measures

Primary Outcomes (1)

• Total RNA in muscle tissue

  Total RNA content in m. vastus lateralis (ug per mg tissue)

  Before the intervention and immediately after the intervention (i.e. after 5 training sessions of each leg)

Secondary Outcomes (14)

• Ribosomal RNA in skeletal muscle

  Before the intervention and immediately after the intervention (i.e. after 5 training sessions of each leg)

• Protein in skeletal muscle

  Before the intervention and immediately after the intervention (i.e. after 5 training sessions of each leg)

• Gene expression in skeletal muscle

  Before the intervention and immediately after the intervention (i.e. after 5 training sessions of each leg)

• Muscle fractional synthesis rate

  Immediately after the intervention

• Glucose in blood, after glucose/placebo intake

  Immediately before glucose/placebo intake and 30 min, 45 min, 60 min and 195 min after initial glucose/placebo intake

Other Outcomes (7)

• Training diary

  During each training session of the intervention

• Body mass composition

  Prior to the intervention

• Dietary registration

  During each day of the intervention

Study Arms (2)

Glucose

ACTIVE COMPARATOR

Glucose will be ingested at three time points during resistance training (RT): 30 min prior to RT (30 g glucose mixed with 300 ml sugar-free Fun light lemonade), immediately prior to RT (30 g, 300 ml), and immediately after completion of training (30 g, 300 ml). Protein supplement will be ingested at two time points: 2 hours prior to RT (e.g. at 0700 hrs, 25 g) and immediately after completion of training (25 g). Placebo will be ingested during the afternoon (i.e. not during training; between 1800 hrs and 1900 hrs): 3 x 100 mg Stevia powder mixed with 3 x 300 ml sugar-free Fun light lemonade. (The dietary intervention spans from 2200 hrs on the evening prior to RT sessions to \~2.5 hrs after completion of RT. During this time frame, participants will ingest glucose and protein supplements only)

Dietary Supplement: Glucose

Placebo

PLACEBO COMPARATOR

Placebo will be ingested at three time points during resistance training (RT): 30 min prior to RT (100 mg Stevia powder mixed with 300 ml sugar-free Fun light lemonade), immediately prior to RT (100 mg, 300 ml), and immediately after completion of training (100 mg, 300 ml). Protein supplement will be ingested at two time points: 2 hours prior to RT (e.g. at 0700 hrs, 25 g) and immediately after completion of training (25 g). Glucose will be ingested during the afternoon (i.e. not during training; between 1800 hrs and 1900 hrs): 3 x 30 g glucose mixed with 3 x 300 ml sugar-free Fun light lemonade. (The dietary intervention spans from 2200 hrs on the evening prior to RT sessions to \~2.5 hrs after completion of RT. I.e.: during this period, participants will ingest placebo and protein supplements only)

Dietary Supplement: Glucose

Interventions

Glucose DIETARY_SUPPLEMENT

To investigate the effects of glucose intake during resistance training on muscle biological adaptations

Glucose; Placebo

Eligibility Criteria

• Age: 20 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Non-smoking
• Moderately trained (i.e. having performed 2-8 resistance training sessions per 14 days for the last six months)

You may not qualify if:

• Not able to understand Norwegian
• Unstable cardiovascular disease
• Illness or injury contradicting heavy strength training
• Disabling musculoskeletal disease
• Serious mental illness
• Allergy to local anaesthesia
• Impaired glucose tolerance

Sponsors & Collaborators

• Inland Norway University of Applied Sciences: lead

Study Sites (1)

Inland Norway University of Applied Sciences

Lillehammer, Norway

Location

Related Publications (1)

• Hammarstrom D, Ofsteng S, Koll L, Hanestadhaugen M, Hollan I, Apro W, Whist JE, Blomstrand E, Ronnestad BR, Ellefsen S. Benefits of higher resistance-training volume are related to ribosome biogenesis. J Physiol. 2020 Feb;598(3):543-565. doi: 10.1113/JP278455. Epub 2020 Jan 15.

  PMID: 31813190 BACKGROUND

MeSH Terms

Interventions

Glucose

Intervention Hierarchy (Ancestors)

Hexoses; Monosaccharides; Sugars; Carbohydrates

Study Officials

• Stian Ellefsen, PhD

  Inland Norway University of Applied Sciences

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Masking Details: On each intervention day, participants will receive boluses of supplements in accordance with his or her study ID number. The list that links this ID number to the randomization code will be kept with the person who generated the randomization code (and stored on a safe server) until completion of data sampling and cleaning of data on main outcome measures. The person resonsible for generating the randomization code will not be involved in any aspects of data sampling or handling. None of the project collaborators/participants will have access to this list during the intervention or during data handling. Half the participants (n=10) will commence the intervention with GLU on Day 1, while the other half will commence with PLAC (randomized). For participants starting with GLU, half will perform training on their dominant leg, while the other half will perform training on their non-dominant leg. The same will be the case for participants starting with PLAC.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Administrator

Model Details: The study will be conducted as a 12-day placebo-controlled randomized clinical trial. Each day will consist of concomitant dietary intervention and resistance training. The dietary intervention consists of alternating days of ingesting glucose (GLU, 90 g) or placebo (PLAC) in connection with training. Likewise, training consists of alternating days of resistance training of the two legs (using identical training protocols), with Day 1 involving training of the first leg, Day 2 involving training of the second leg, Day 3 involving training of the first leg, etc. In this way, each of the two dietary interventions will be associated with training of one particular leg, allowing within-subject comparisons of the effects of GLU vs PLAC on muscle biology and recovery.

Study Record Dates

• First Submitted: August 24, 2020
• First Posted: September 10, 2020
• Study Start: September 1, 2020
• Primary Completion: December 20, 2020
• Study Completion: December 20, 2020
• Last Updated: February 4, 2021

Record last verified: 2021-02

Data Sharing

• IPD Sharing: Will not share

Locations

NO (1)