Exercise Dose-response and Protein Requirements

NCT06138951 | Completed

• 1 other identifier: DRE
• Study Type: interventional
• Target: 7
• Locations: 1 country
• Sites: 1

Brief Summary

Study will investigate the impact of training volume on protein metabolism to estimate the impact on daily protein requirements of endurance athletes.

Conditions

Exercise Training

Outcome Measures

Primary Outcomes (1)

• Phenylalanine excretion

  Excretion of 13CO2 in the breath will be used to estimate the impact of exercise on daily protein requirements. This outcome will include the enrichment of 13CO2 in exhaled breath after the ingestion of \[13C\]phenylalanine tracer and the production of CO2 determined by indirect calorimetry at rest or after exercise.

  8 hours

Secondary Outcomes (2)

• Phenylalanine flux

  8 hours

• Phenylalanine oxidation

  8 hours

Study Arms (3)

Nonexercised rested day

PLACEBO COMPARATOR

Athlete will not exercise on trial day.

Other: Endurance exercise

Recovery from 10KM run

EXPERIMENTAL

Athlete will run 10km on trial day.

Other: Endurance exercise

Recovery from 20KM run

EXPERIMENTAL

Athlete will run 20km on trial day.

Other: Endurance exercise

Interventions

Endurance exercise OTHER

On the trial day before commencing the indicator amino acid oxidation metabolic trial, participants will either rest, perform a 10km run on a treadmill, or perform a 20km run on a treadmill.

Nonexercised rested day; Recovery from 10KM run; Recovery from 20KM run

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy, male, endurance-trained participants who regularly run more than 40 km/week or perform endurance exercise for more than 4.5 hours/week
• Participants who have a self-reported 5-km run time of ≤23 min
• Participants who are categorized as at least "very good" based on a study by Shvartz \& Reibold (Aviat Space Environ Med. 1990 Jan;61(1):3-11), in which VO2peak is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y)
• Ability to complete the 16-km familiarization run on Session 2
• Aged 18-40 years old

You may not qualify if:

• \- Inability to meet health and physical activity guidelines according to the PAR-Q+.
• Female: hormonal fluctuations associated with the menstrual cycle have been reported to alter protein metabolism during exercise and may influence specific amino acid requirements at rest; as such, the present study will test the effect of endurance exercise on protein requirements in males only to ensure a stable hormonal environment and to increase the homogeneity of the physiological response. (NOTE: we plan to test females in a future trial based off of the results obtained herein)
• Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
• Regular tobacco use
• Illicit drug use (e.g. growth hormone, testosterone, etc.)

Sponsors & Collaborators

• University of Toronto: lead

Study Sites (1)

Goldring Centre for High Performance Sport at the University of Toronto

Toronto, Ontario, M5S2C9, Canada

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: November 9, 2023
• First Posted: November 18, 2023
• Study Start: November 17, 2017
• Primary Completion: August 15, 2018
• Study Completion: January 1, 2019
• Last Updated: January 5, 2024

Record last verified: 2024-01

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)