The Effect of Exercise and Presleep Nutrition on Muscle Connective Tissue Remodelling

NCT06054620 | Unknown

• 1 other identifier: CVC
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

The primary objective of this study will be to determine the influence of a presleep vitamin C-enriched, collagen-modelled supplement (CVC) on the metabolic fate of dietary glycine using a \[2H5\]glycine tracer within the intramuscular connective tissue at rest and after a bout of resistance exercise. Other outcomes will be related to regulation collagen remodelling in skeletal muscle.

Conditions

Interventional

Keywords

Collagen; Vitamin C; Carbohydrate placebo

Outcome Measures

Primary Outcomes (1)

• Enrichment of Muscle Collagen

  Overnight incorporation of orally ingested amino acid tracer (D5 Gly and D5 Phe) into mature collagen protein isolated from skeletal muscle biopsy samples taken 9 hours after consumption of the tracer-containing supplement (CVC or PLA) and rest or resistance exercise. Enrichment will be quantified via tandem LC-MS/MS.

  9 hours

Secondary Outcomes (3)

• Area of muscle with localized collagen remodelling

  9 hours

• Concentration of Urinary Growth Hormone

  8 hours

• Enrichment of Myofibrillar Protein

  9 hours

Other Outcomes (1)

• Intensity of Muscle Soreness

  24 hours

Study Arms (2)

Collagen and Vitamin C

ACTIVE COMPARATOR

Dietary Supplement: Collagen and Vitamin C

Carbohydrate Placebo

PLACEBO COMPARATOR

Dietary Supplement: Carbohydrate Placebo

Interventions

Collagen and Vitamin C DIETARY_SUPPLEMENT

Collagen-modelled crystalline amino acids (30g total, 8g glycine) and vitamin C (500 mg).

Also known as: CVC

Collagen and Vitamin C

Carbohydrate Placebo DIETARY_SUPPLEMENT

Iso-caloric Placebo (30g maltodextrin).

Carbohydrate Placebo

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy, recreationally active, male and female participants.
• Healthy will be defined as someone who does not have a health condition that would compromise their ability to safely participate in the strenuous physical activity involved in our study as screened for by the Get Active Questionnaire.
• Willing to abstain from resistance exercise for 2 weeks prior to the first metabolic trial and for the duration of the study.
• Participants must be able to abstain from lower-body resistance and/or plyometric exercise for at least 2 weeks prior to the first metabolic trial.
• Participants will be aged 18-35 years old.
• Participants must be normal to overweight (e.g., BMI 18.5-30).
• Participants are willing to abide by the compliance rules of this study (e.g., abstain from physical activity and alcohol 48h prior to each trial).
• Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).

You may not qualify if:

• Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor.
• Self-reported regular tobacco use.
• Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
• Regular use of anti-inflammatory drugs (e.g., ibuprofen).
• Self-reported regularly sleeping less than an average of 7 hours over 3 days (minimum recommended daily amount).
• Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
• Use of birth control and discontinued use in the last 3-months (female only).

Sponsors & Collaborators

• University of Toronto: lead

Study Sites (1)

Goldring Centre for High Performance Sport at the University of Toronto

Toronto, Ontario, M5S 2C9, Canada

RECRUITING

MeSH Terms

Interventions

Collagen; Ascorbic Acid

Intervention Hierarchy (Ancestors)

Biopolymers; Polymers; Macromolecular Substances; Extracellular Matrix Proteins; Scleroproteins; Proteins; Amino Acids, Peptides, and Proteins; Sugar Acids; Acids, Acyclic; Carboxylic Acids; Organic Chemicals; Hydroxy Acids; Carbohydrates

Central Study Contacts

Daniel R Moore, PhD

CONTACT

416-946-4088; dr.moore@mail.utoronto

Paul L Babits, BKin

CONTACT

647-609-4710; paul.babits@mail.utoronto.ca

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Masking Details: Double blinded (investigators and participants). A lab member external to the investigation will code items for the intervention.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Model Details: Crossover to receive Collagen and Vitamin C (CVC) or Carbohydrate placebo. Unilateral leg exercise (EX) to give 4 outcomes. (1) CVC-EX; (2) CVC-REST; (3) PLACEBO-EX; (4) PLACEBO-REST

Study Record Dates

• First Submitted: September 19, 2023
• First Posted: September 26, 2023
• Study Start: September 15, 2023
• Primary Completion: September 1, 2024
• Study Completion: August 1, 2025
• Last Updated: November 13, 2023

Record last verified: 2023-11

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)