In Vivo and in Vitro Anabolic Potential of Essential Amino Acids Following Resistance Exercise

NCT05754125 | Unknown

• 1 other identifier: DiEx
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

This study seeks to investigate the anabolic potential of a dileucine-enriched essential amino acid (EAA) formulation compared with a branched chain amino acid (BCAA) alternative and a collagen beverage on muscle protein anabolism and catabolism following a bout of resistance exercise training. To do this, investigators will employ a novel 'breath test' method developed in our laboratory as well as blood and urine sampling. The results of this study will allow us to better understand the anabolic potential of dileucine which could have implications for people engaging in regular resistance training (such as athletes) as well as people that need to preserve muscle mass (older people who are susceptible to anabolic resistance and sarcopenia, or muscle wasting).

Conditions

Interventional

Keywords

Di-leucine; Branched chain amino acids; Collagen; Amino acids; anabolism; catabolism

Outcome Measures

Primary Outcomes (1)

• Whole-Body Protein Synthesis

  Investigators will measure the enrichment of \[13CO2\] in the breath by isotope ratio mass spectrometry (IRMS) in atom percent excess (APE). The measurement of carbon dioxide production (VCO2) and stable isotope tracer enrichment in the breath allows for the assessment of the rate at which amino acids are used for energy (i.e., oxidized), rather than for protein synthesis (i.e., retained in the body) by calculating the fraction of expired CO2 that contains 13C. Leucine retention (umol/kg) will then be calculated from the difference between the known amount of leucine provided (ingested) and leucine oxidation (as determined from 13CO2 breath enrichment).

  6 hours

Secondary Outcomes (4)

• Urinary Measures (Muscle Protein Breakdown)

  6 hours

• Murine Cell-Based Experiments (ex-vivo experiments) -hypertrophy

  60 minutes

• Murine Cell-Based Experiments (ex-vivo experiments) - Protein Synthesis

  60 minutes

• Murine Cell-Based Experiments (ex-vivo experiments) - mTORC1 Signalling

  60 minutes

Study Arms (3)

Di-Leucine supplement

EXPERIMENTAL

Participants will consume a amino acid supplement containing Di-Leucine following resistance exercise and their whole body anabolism will be determined over the subsequent 6 hours

Dietary Supplement: Di-Leucine Supplement

BCAA Supplement

EXPERIMENTAL

Participants will consume a amino acid supplement containing branched chain amino acids (BCAA) following resistance exercise and their whole body anabolism will be determined over the subsequent hours

Dietary Supplement: BCAA Supplement

Collagen Supplement

EXPERIMENTAL

Participants will consume a amino acid supplement containing collagen protein following resistance exercise and their whole body anabolism will be determined over the subsequent hours

Dietary Supplement: Collagen Supplement

Interventions

Di-Leucine Supplement DIETARY_SUPPLEMENT

Participants will consume a amino acid supplement containing Di-Leucine following resistance exercise and their whole body anabolism will be determined over the subsequent 6 hours

Di-Leucine supplement

BCAA Supplement DIETARY_SUPPLEMENT

Participants will consume a amino acid supplement containing branched chain amino acids (BCAA) following resistance exercise and their whole body anabolism will be determined over the subsequent hours

BCAA Supplement

Collagen Supplement DIETARY_SUPPLEMENT

Participants will consume a amino acid supplement containing Collagen protein following resistance exercise and their whole body anabolism will be determined over the subsequent hours

Collagen Supplement

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Trained individuals currently performing structured exercise (e.g., running, weightlifting, team-sport activity) at least 2 days per week for the previous 3 months

You may not qualify if:

• Inability to perform physical activity as determined by the PAR-Q
• Inability to adhere to protocol guidelines (e.g., alcohol, habitual diet)
• Regular tobacco use
• Illicit drug use (e.g., growth hormone, testosterone, etc.)
• Diagnosed medical condition under the care of a physician (e.g. type 2 diabetes)
• Inability to abstain from supplements (e.g. protein, creatine, HMB, BCAA, phosphatidic acid, etc.) at least three weeks before the trial
• Individuals on any medications known to affect protein metabolism (e.g., corticosteroids, non-steroidal anti-inflammatories, or prescription-strength acne medications)
• Participants that are amenorrheic (females only)
• On medications that may interfere with protein metabolism (e.g. anti-inflammatory drugs, hormone therapy)
• Regular tobacco use (e.g. daily use of oral or inhaled tobacco)
• Illicit drug use (e.g. growth hormone, testosterone, etc.)
• Inability to comply with the study protocol as judged by the investigators

Sponsors & Collaborators

• University of Toronto: lead

Study Sites (1)

Goldring Centre for High Performance Sport at the University of Toronto

Toronto, Ontario, M5S2C9, Canada

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: Neither participants, investigators, or data analysts will be aware of the supplement assignment (labelled A-C) until data analysis for the primary outcome is complete.
• Purpose: TREATMENT
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Model Details: Cross over study design; Block randomized.

Study Record Dates

• First Submitted: January 31, 2023
• First Posted: March 3, 2023
• Study Start: January 21, 2023
• Primary Completion: July 30, 2023
• Study Completion: January 30, 2024
• Last Updated: November 9, 2023

Record last verified: 2023-11

Locations

CA (1)