Genetics of Ischemic Cardiomyopathy

NCT05508269 | Completed

• 1 other identifier: RHFCS1
• Study Type: observational
• Target: 26
• Locations: 1 country
• Sites: 1

Brief Summary

Background:Ischemic heart disease is one of the heaviest health-related burdens worldwide.We aimed to identify the common hub mRNA and pathways that are involved in pathological progression of ischemic cardiomyopathy. Methods: To explore potential differentially expressed genes (DEGs) of all ischemic heart disease stages, we used chipster and GEO2R tools to analyze of retrieved eight high throughput RNA datasets obtained from GEO database. Gene Ontology functional annotation and Pathways enrichment analyses were used to obtain the common functional enriched DEGs which were visualized in protein-protein interactions (PPI) network to explore the hub mRNA according to the interaction scores. Validation qRT-PCR was carried out for blood and cardiac biopsies compared with controls to validate the determined four hub mRNAs and subsequently reviewed inside comprehensive published meta-analysis database. The validated mRNAs were visualized in two interaction modules. Finally screening of approved drugs was applied.

Conditions

Ischemic Cardiomyopathy

Keywords

Ischemic Cardiomyopathy; qrt-pcr

Outcome Measures

Primary Outcomes (1)

• Genetic pathway of pathological progression from stable coronary artery disease to myocardial infarction and finally to ischaemic cardiomyopathy

  The expected results may explain novel genetic pathways of the clinical progression of asymptomatic structural cardiac disorder (Stable coronary artery disease) to typically symptomatic ischaemic cardiomyopathy. Gene expression profiling of seven genes in RNA of all cardiac specimens was measured using QPCR analysis.

  2 years

Study Arms (4)

Control subjects

control cases who were diagonosed as healthy subjects after diagnostic catheterization

Genetic: quantitative polymerase chain reaction(QPCR) for four genes; Procedure: Cardiac biopsy, blood sampling

Stable coronary artery disease

patients with stable coronary artery disease undergoing elective PCI who were diagnozed with coronary lesion/s and underwent stent implantation without previous history of myocardial infarction

Genetic: quantitative polymerase chain reaction(QPCR) for four genes; Procedure: Cardiac biopsy, blood sampling

Myocardial infarction patients

patients with history of myocardial infarction or with acute myocardial infarction undergoing primary PCI

Genetic: quantitative polymerase chain reaction(QPCR) for four genes; Procedure: Cardiac biopsy, blood sampling

Ischaemic cardiomyopathy patients

patients with ischaemic cardiomyopathy diagnosed by ejection fraction less than 35% with history of coronary artery disease or myocardial infarction or those with acute myocardial infarction with reduced ejection fraction.

Genetic: quantitative polymerase chain reaction(QPCR) for four genes; Procedure: Cardiac biopsy, blood sampling

Interventions

quantitative polymerase chain reaction(QPCR) for four genes GENETIC

quantitative polymerase chain reaction(QPCR) for four genes

Control subjects; Ischaemic cardiomyopathy patients; Myocardial infarction patients; Stable coronary artery disease

Cardiac biopsy, blood sampling PROCEDURE

In the studied thirteen peripheral blood subjects, five milliliters blood samples were taken from each patient in potassium EDTA tubes, immediately inserted in liquid nitrogen container and then stored in -80 refrigerators. All studied thirteen cardiac tissue subjects were underwent cardiac biopsy after PCI in order to take two specimens from the left ventricle using judkin right seven french catheters for femoral access and cardiac bioptome 2.3 mm wide. All cardiac specimens were collected in cryotubes, immediately inserted in liquid nitrogen container and then stored in -80 refrigerators.

Control subjects; Ischaemic cardiomyopathy patients; Myocardial infarction patients; Stable coronary artery disease

Eligibility Criteria

• Age: 18 Years - 80 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Study subjects were categorized into three control cases who were diagonosed as healthy subjects after diagnostic catheterization, three patients with stable coronary artery disease undergoing elective PCI who were diagnozed with coronary lesion/s and underwent stent implantation without previous history of myocardial infarction, four patients with history of myocardial infarction or with acute myocardial infarction undergoing primary PCI and three patients with ischaemic cardiomyopathy diagnosed by ejection fraction less than 35% with history of coronary artery disease or myocardial infarction or those with acute myocardial infarction with reduced ejection fraction.

Sponsors & Collaborators

• Cairo University: lead

Study Sites (1)

cardiology hospital (Asyut University, Asyut, Egypt)

Asyut, Egypt

Location

Biospecimen

Retention: SAMPLES WITH DNA

Cardiac tissue specimens and blood specimens

MeSH Terms

Interventions

Blood Specimen Collection

Intervention Hierarchy (Ancestors)

Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Investigative Techniques

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: PhD Mina Wageh Mohareb, Clinical researcher, Faculty of Pharmacy, Cairo University

Study Record Dates

• First Submitted: September 13, 2019
• First Posted: August 19, 2022
• Study Start: April 1, 2021
• Primary Completion: August 17, 2022
• Study Completion: August 17, 2022
• Last Updated: January 26, 2023

Record last verified: 2023-01

Data Sharing

• IPD Sharing: Will not share

Locations

EG (1)